Reagent. Shake the
96-well plate and then incubate the reaction mixture
for another 40 minutes at ambient temperature.
6. Then add 50 µl of the Kinase Detection Reagent to
the 96-well plate and incubate
tested. Both infection
conditions produced peak TCID50 titers in the range of
108 - 109 particles/mL between 72 - 96 hours post-infection
(Figure 4).
Summary
EX-CELLTM MDCK is a serum-free medium developed
cells® as
above. After three days of growth at 37°C and 5% CO2, the spinners
were infected with 3 x 109 virus particles (moiª3) of the rAd5 containing
the ß-galactosidase (ß-gal) reporter gene (Crucell
3 of 3
09/03/96 - TMG
PHOSPHATASE, ALKALINE
from Bovine Intestinal Mucosa
Sigma Prod. No. P5778
REFERENCES:
1. Neumann, H. and Lustig, A., European Journal of Biochemistry, 109, 475-480 (1980
Mater. Res., 61(1),
109-120 (2002).
3. Navarro, J. M., et al., Ammonium, bicarbonate and
calcium effects on tomato plants grown under
saline conditions. Plant Sci., 157(1), 89-96 (2000).
4. Kuberan
c
e
104 105 106 107 108 109 1010-5 -4 -3 -2 -1 0 1 2
0.0
250000.0
500000.0
750000.0
1000000.0
1250000.0
lu
m
in
e
s
e
n
c
e
104 105 106 107 108 109 1010
1
4. Carefully transfer 24 L of supernatant into a clean
tube.
5. Add 96 L of Assay Buffer. Label this tube “HDL”.
6. Carefully remove all remaining supernatant from
the pellet.
7. Transfer
References:
1. S. Endo, Über ein Verfahren zum Nachweis von Typhusbacillen, Centralbl. Bakt. I. Orig., 35, 109
(1904)
2. G. Naundorf, N.G. Aumen, The assessment of ammonia-induced cell envelope injury in E
noncell-based permeability assays. Journal of the
Association for Laboratory Automation 12(2):104–109.
http://journals.sagepub.com/doi/pdf/10.1016/
j.jala.2006.10.008
3. Wohnsland, F. and Faller
contains the
receptor-binding domain and a potential N-linked
glycosylation site at amino acid residue 109.
Human TRAIL shares about 65% amino acid sequence
homology with mouse TRAIL and is active on mouse
Bernharroch, D., et al., Isr. J. Med. Sci., 26, 212
(1990).
10. Moscatelli, H., et al., J. Cell Biol., 109, 2519
(1989).
11. Mansukhani, A., et al., Proc. Natl. Acad. Sci. USA,
87, 4378 (1990).
12. Thomas
Xanthine Standards for Colorimetric Detection
Add 0, 2, 4, 6, 8, and 10 µL of the 2 mM (2 nmole/µL)
Xanthine Standard Solution into a 96 well plate in
duplicate, generating 0 (blank), 4, 8, 12, 16