concentration of 6 × 105 cells/mL.
• Perform minimum 3 passages (100 mL in 250 mL
shaker bottles at 110 rpm). Passage when VCD
(viable cell density) is above 1.5 × 106 cells/mL
• When the culture is
Layer (BOT)
Color of Progressing Front: Blue
Adhesive: 2641, Pressure sensitive, -40°C to +110°C, Finger-
lift – see Appendix 1
Product drawing: See Fig. 2
Graphics:
Practical Guide,
Passonneau, J.V., and Lowry, O.H., The Humana
Press, Inc., (Totowa, NJ:1993), 85-110.
5. The Merck Index, 13th, Entry# 6370.
6. Data for Biochemical Research, 3rd ed., Dawson,
R.
expressed in human HEK 293 cells as
a glycoprotein with a calculated molecular mass of
72 kDa (amino acids 110-660). The DTT-reduced
protein migrates as a 80-75 kDa polypeptide on
SDS-PAGE because
expressed in human HEK 293 cells as
a glycoprotein with a calculated molecular mass of
72 kDa (amino acids 110-660). The DTT-reduced
protein migrates as a 80-75 kDa polypeptide on
SDS-PAGE because
Top Layer (BOT)
Color of Progressing Front: Blue
Adhesive: 2641, Pressure sensitive, -40°C to +110°C, Finger-
lift – see Appendix 1
Product drawing: See Fig. 2
Graphics: See Fig. 3
Other: Not
Top Layer (BOT)
Color of Progressing Front: Blue
Adhesive: 2641, Pressure sensitive, -40°C to +110°C, Finger-
lift – see Appendix 1
Product drawing: See Fig. 2
Graphics: See Fig. 3
Other: Not
(µl)
0.5 12.5
1 20
2 40
4 55
6 70
8 110
3. Add the DNA solution to the diluted NeuroPorter
Transfection reagent. Incubate the mixture at
room temperature for 5 to 10 minutes to allow the
formation
250 mL, plastic with vent filter) and a shaker
incubator (37 °C, 80% relative humidity, 5% CO2, agitation at 110 rpm). The use of CO2-
controlled conditions is important in serum-free culture since the
Layer (BOT)
Color of Progressing Front: Blue
Adhesive: 2641, Pressure sensitive, -40°C to +110°C, Finger-
lift – see Appendix 1
Product drawing: See Fig. 2
Graphics:
Layer (BOT)
Color of Progressing Front: Blue
Adhesive: 2641, Pressure sensitive, -40°C to +110°C, Finger-
lift – see Appendix 1
Product drawing: See Fig. 2
Graphics:
Layer (BOT)
Color of Progressing Front: Blue
Adhesive: 2641, Pressure sensitive, -40°C to +110°C, Finger-
lift – see Appendix 1
Product drawing: See Fig. 2
Graphics: