database, accession number
P06400). The sequence is conserved in human, mouse
(80% homology) and rat (87 % homology). The
antiserum is preadsorbed to remove any reactivity with
non-phosphorylated Rb protein
196
FBS 110 97 76 62 62 83 100 112
Human 92 77 61 49 47 64 81 93
10 www.millipore.com
Spin Sample Average Ultrafiltrate Volume (µL) by Row
Time Type A B
vascular endothelial cell mitogen from a rat
glioma-derived cell line. Proc. Natl. Acad. Sci. USA,
87, 1323-1327 (1990).
IDC,KAA,MAM 06/12-1
2012 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH
vascular endothelial cell mitogen from a rat glioma-
derived cell line, Proc. Natl. Acad. Sci. USA, 87,
1323-1327 (1990).
KAA 05/01
Sigma brand products are sold through
amebocyte lysate) method.
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for at least 2 passages prior to sub-culturing in
Cellvento™ CHO-
in Cellvento® CHO-110
medium should be thawed and maintained in Cellvento® CHO-110 growth medium for at least
2 passages prior to sub-culturing in Cellvento® CHO-210 medium.
2. Sequential media adaptation
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5.1 References
1 Alnemri E.S. et al. (1996) Human ICE/CED-3 Protease Nomenclature.
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2 Casciola-Rosen et al. (1996) Apopain/CPP32 Cleaves Proteins That Are
Essential for Cellular Repair