polyacrylamide gel produces two labeled
bands: a 33 bp oligonucleotide band (residual
uncleaved substrate) and a 15 bp nucleotide band (the
cleavage product).
Assay
1. Prepare 20% denaturing gel containing
dilutions by titration test.
References
1. Rosnet, O., and Birnbaum, D., Crit. Rev. Oncog. 4,
595-613 (1993).
2. Rosnet, O., et al., D., Blood, 82, 1110-1119 (1993).
3. Small, D., et al., Proc. Natl