mm 28 x 23 / 22 24 III (FL) 3x400/50/16 59 x 76 x 116 156 9 352 752 FP52-SL
0.4-0.7 0.2-0.4 22-26 M16x1 8 / 12 mm 28 x 23 / 22 24 III (FL) 3x400/50/16 59 x
and reproducibility of the method at the 5 ng/g spiking level was
good, with average recoveries for spiked replicates between 85 and 116%,
and RSDs 5% for all three matrices (Table 2). No internal
synthetic phosphorylated peptide derived from the
human eNOS sequence, corresponding to the serine
116 phosphorylation site as immunogen. The
antiserum is affinity purified using epitope-specific
affinity
DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM dithiothreitol, 1 mM ATP, pH 7.5 (at 20°C) result-
ing
supernatant that has
been isolated after centrifuging a resuspension of the
Sephadex® beads in water at 116 mg beads per 1 mL
of water, with overnight incubation at 2-8 °C. Small
aliquots of the Sephadex®
lymphocytes evaluated by the
comet assay and cytogenetic tests. J. Trace
Elem. Med. Biol., 16(3), 187-192 (2002).
5. Fatur, T., et al., Cadmium inhibits repair of UV-,
methyl methanesulfonate- and N-methyl-N
Version: 10Biotin-16-ddUTP
sigma-aldrich.com 5
2.3. Parameters
Chemical Formula
C32 H48 N7 O17 P3 SLi4
Chemical Name
Structural formula
Fig. 1: Chemical structure of Biotin-16-ddUTP.
Molecular
the protein backbone of BSM, following acid treatment
to remove glycans, in the range of 108-116 kDa.5
The protein backbone of BSM is notably rich in Pro,
Ser, Thr, and Gly residues.6 Several molecular
,
Endocrinology, 116, 359-363 (1985).
3. Hoffman, T. et al., Glycosylation of human
prolactin regulates hormone bioactivity and
metabolic clearance, J. Endocrinol. Invest.,
16, 807-816 (1993).
excess Ca
2+
to the Ca
2+
-free enzyme.
16
Inhibitors: Proteinase K is inhibited by DIFP or PMSF
(the latter used at final concentration 5 mM).
3
It is