standard passes
Melting range, °C
Class 1b 〈741〉
between 115° and 119° 117-119°
Specific rotation, [α]D
25
15 mg/mL in alcohol 〈781S〉
between +103° and +106° +105°
Reducing substances
splenocytes from a
BALB/c mouse immunized with a synthetic peptide cor-
responding to amino acids 119-133 of human
caspase 4 protein, conjugated to KLH. The isotype is
determined using Sigma ImmunoTypeTM
A. N., and Wassermann, R. H, Arch.
Biochem. Biophys., 119, 536 (1967).
2. Kretsinger, R. H., CRC Crit. Rev. Biochem., 8, 119
(1980).
3. Garcia-Segura, L. M., et al., Brain Res., 296, 75
(1984
to standard passes
Melting range, °C
Class 1b 〈741〉
between 115° and 119° 115°-117°
Specific rotation, [α]
D
25
15 mg/mL in alcohol 〈781S〉
between +103° and +106° +104°
Reducing substances
Materials Required But Not Provided
Solution A Solution B
Wash Buffer Wash Buffer plus Ca2+
119 mM NaCl (S5886) 119 mM NaCl (S5886)
2.5 mM KCl (P5405) 2.5 mM KCl (P5405)
4 mM MgCl2 (M2393) 4 mM MgCl2 (M2393
Immunogen: Recombinant protein Dual adaptor of phos-
photyrosine and 3-phosphoinositides
Immunogen Sequence: GI # 7657007, sequence 119 - 164
The product is a clear, colorless solution in phosphate
buffered
17%): B (17%)
A (17%): C (67%): B (17%) A (17%): C (17%): B (67%)
6=119%
3=114%
2=115%
1=100%
A
B C
4=142%
5=119%
10=129%
9=120%8=135%
7=141%
Fig. 5 Criteria =
internal standard for
bioanalysis of B2M in mass spectrometry. SILuB2M is
a monomer of 119 amino acids (including C-terminal
polyhistidine and FLAG
tags), with a calculated
molecular mass
Profilin 2 is the neuronal specific
isoform and profilin 3 is a testis specific isoform.2, 3
Profilins of eukaryotic cells are small (12-15 kDa)
cytoplasmic proteins that bind to actin monomers,
polyphosphoinositides
Biochemistry, 30, 278-286 (1991).
3. Santarpia, L. et al., Targeting the MAPK-RAS-RAF
signaling pathway in cancer therapy. Expert Opin.
Ther. Targets, 16(1),103-119 (2012).
FLAG is a registered