offer protection from
light.
6. For loading with DiOC2(3), incubate for 15 min on ice. For loading with
Rhodamine 123, incubate for 30 min to 2 h on ice.
7. Centrifuge cells at 200 x
°C 4 to 37 °C
Sterilization Autoclave for two cycles of 60 minutes at 123 °C
Autoclave for one cycle of 60 minutes at
123 °C
Typical Flush/Process Flux 100 to 600 LMH
Maximum
Differential
Pressure
Sterilization
µPod® Autoclave for two cycles of 60
minutes at 123 °CLab Scale
Pod Autoclave for one cycle of 60 minutes at 123 °C
Typical Process Flux 100 to 600 LMH
Maximum
Differential
14 mm (9/16 in.) hose barb outlet
HH = 14 mm (9/16 in.) hose barb inlet and outlet
* Filters were tested post gamma radiation at 45-65 kGy
and autoclaved at 123 °C for 60
,
10759-10761 (1994).
5. Tsukita S., et al., J. Cell Biol., 123, 1049-1053
(1993).
6. Bradley, R., et al., J. Cell Biol., 123, 1857-1865
(1993).
7. Hinck, L., et al., J. Cell Biol.
Autoclaving Water wet filter for 5 minutes at 3.4 bar (50 psi), liquid cycle, slow exhaust, at 123 °C maximum for 60
minutes.
Bacteriophage Retention Lot release testing on samples exhibited ≥ 4
glycerol.
Molecular mass: ∼85 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 91–123 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
been shown to retard glial
proliferation at 15 µg/ml.8 An 8.47% solution is iso-
osmotic with serum and causes 3% hemolysis of
erythrocytes after 45 minutes.9
Precautions and Disclaimer
For Laboratory
, J. Biol. Chem., 273, 12219-12226
(1998).
ProductInformation
15. Sato, Y., et al., Br. J. Pharmacol., 123, 97-105
(1998).
16. Easom, R. A., et al., Biochem. J., 329, 283-288
(1998).
, J. Biol. Chem., 273, 12219-12226
(1998).
ProductInformation
15. Sato, Y., et al., Br. J. Pharmacol., 123, 97-105
(1998).
16. Easom, R. A., et al., Biochem. J., 329, 283-288
(1998).
(1998).
14. Pahan, K. et al., J. Biol. Chem., 273, 12219-12226
(1998).
15. Sato, Y. et al., Br. J. Pharmacol., 123, 97-105
(1998).
16. Easom, R.A. et al., Biochem. J., 329, 283-288
(1998).