practices.
References
1. Hogan, B., et al., Manipulating the Mouse Embryo,
A Laboratory Manual, 2nd Edition, Cold Spring
Harbor Press (Cold Spring Harbor, NY: 1994)
pp. 142-143. (Product Number
with 5% CO2.
References
1. Liu, Y. et al., Disulfide-crosslinked Hyaluronan-
Gelatin Sponge: Growth of Fibrous Tissue In Vivo,
J. Biomed. Mat. Res., 68A, 142-149 (2004).
2. Liu, Y. et al.
(C. Avoid repeated
freezing and thawing.
References
1. Staeckle, M., et al., New Biol., 2, 313 (1990).
2. Brown, K., et al., J. Immunol., 142, 679 (1989).
3. Schall, T., et al., Nature, 347, 669 (1990
within each well of a 96 well plate)
• Reconstitute reagent in 50µL of sterile nuclease free
water.
• Create a working solution based on your experiment
by diluting 1:20 in sterile PBS.
•
histidyl-tRNA synthetase. Biodesign, 002(04), 142-
148 (2014).
Trizma is a registered trademark of Sigma-Aldrich Co.
LLC
GCY,RBG,MAM 04/18-1
2018 Sigma-Aldrich Co. LLC. All rights
colorectal cancer. Nat.
Cancer, 1(3), 345-358 (2020).
3. Ma, H., et al (2018). Inhibition of SLC1A
5 sensitizes colorectal cancer to
cetuximab. Int. J. Cancer, 142(12),
2578-2588 (2018).
lyticase.
REFERENCE:
Scott, J.H. and Schekman, R. (1980) Journal of Bacteriology 142, 414-423
NOTES:
1. YSC-1 substrate solution was found to be stable for up to 8 hours after preparation.
2
lyticase.
REFERENCE:
Scott, J.H. and Schekman, R. (1980) Journal of Bacteriology 142, 414-423
NOTES:
1. YSC-1 substrate solution was found to be stable for up to 8 hours after preparation.
2
lyticase.
REFERENCE:
Scott, J.H. and Schekman, R. (1980) Journal of Bacteriology 142, 414-423
NOTES:
1. YSC-1 substrate solution was found to be stable for up to 8 hours after preparation.
2
Avidin from egg white (A9390) - Product Information Sheet
A9390 Page 1 of 3
11/20/96 - CKV
AVIDIN
from Egg White
Sigma Prod. Nos. A9390 and A9275
CAS NUMBER: 1405-69-2
UNIT DEFINITION
respond to a
mast cell growth-enhancing activity different from
both IL-3 and IL-4. J. Immunol., 142(10),
3440-3446 (1989).
14. Grill, R. J., Jr., and Pixley, S. K.,
2-Mercaptoethanol is a survival
respond to a
mast cell growth-enhancing activity different from
both IL-3 and IL-4. J. Immunol., 142(10), 3440-
3446 (1989).
14. Grill, R. J., Jr., and Pixley, S. K., 2-
Mercaptoethanol is
should be 0.1–1 mg/ml
(1 mg/ml is preferred) for assays performed in 96 well
plates or 2–20 mg/ml for assays performed in cuvettes.
JC-1 Stain
Dissolve the contents of the vial of JC-1 Stain (Catalog
immediately after
the incubation, within 30 minutes.
96-Well Plate Assay
It is recommended to perform the assays in
triplicate.
1. Equilibrate the Substrate Solution and the
Standard Solution
human urine: 10–117 mol methanol/mmol
creatinine; human serum: 32–935 M; human
saliva: 1–142 M. For unknown samples, it is
suggested to perform a pilot experiment and testing
several doses