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Céline Bruyère et al.
Scientific reports, 9(1), 15565-15565 (2019-10-31)
Skeletal muscle fibers are formed by the fusion of mononucleated myoblasts into long linear myotubes, which differentiate and reorganize into multinucleated myofibers that assemble in bundles to form skeletal muscles. This fundamental process requires the elongation of myoblasts into a
Eun Seon Jeong et al.
Colloids and surfaces. B, Biointerfaces, 123, 339-344 (2014-10-08)
This study introduces a drop-based microfluidic approach to physically immobilize liposomes in microgel (liposomes-in-microgel) particles. For this, we generate a uniform liposomes-in-water-in-oil emulsion in a capillary-based microfluidic device. Basically, we have investigated how the flow rate and flow composition affect
Ryan C Hill et al.
Molecular & cellular proteomics : MCP, 14(4), 961-973 (2015-02-11)
The use of extracellular matrix (ECM) scaffolds, derived from decellularized tissues for engineered organ generation, holds enormous potential in the field of regenerative medicine. To support organ engineering efforts, we developed a targeted proteomics method to extract and quantify extracellular
Mohamed G Ayari et al.
Materials science & engineering. C, Materials for biological applications, 101, 254-263 (2019-04-29)
Herein, we propose the synthesis of a microspherical imprinted hydrogel meant for the controlled release of a nucleotide, adenosine 5'-monophosphate (5'-AMP). Indeed, molecularly imprinted polymers-based (MIPs) materials possess remarkable selective molecular recognition ability that mimicks biological systems. MIPs have been
Fei Chen et al.
Science (New York, N.Y.), 347(6221), 543-548 (2015-01-17)
In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently
Leen Bussche et al.
Stem cells translational medicine, 3(12), 1514-1525 (2014-10-15)
Mesenchymal stromal cells (MSCs) have received much attention as a potential treatment of ischemic diseases, including ischemic tissue injury and cardiac failure. The beneficial effects of MSCs are thought to be mediated by their ability to provide proangiogenic factors, creating
Raphael Johannes Morscher et al.
PloS one, 10(6), e0129802-e0129802 (2015-06-09)
Neuroblastoma is a malignant pediatric cancer derived from neural crest cells. It is characterized by a generalized reduction of mitochondrial oxidative phosphorylation. The goal of the present study was to investigate the effects of calorie restriction and ketogenic diet on
Esabi Basaran Kurbanoglu et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 46(2), 631-637 (2015-08-15)
This work addresses the production of prodigiosin from ram horn peptone (RHP) using MO-1, a local isolate in submerged culture. First, a novel gram-negative and rod-shaped bacterial strain, MO-1, was isolated from the body of the grasshopper (Poecilemon tauricola Ramme
Gregory J DiGirolamo et al.
Addictive behaviors, 52, 98-102 (2015-09-26)
Many studies suggest that dependent smokers have a preference or attentional bias toward smoking cues. The purpose of this study was to test the ability of infrequent non-dependent light smokers to control their eye movements by look away from smoking
Anna A Kim et al.
Micromachines, 12(11) (2021-11-28)
Human-induced pluripotent stem cell-derived cardiomyocytes are a potentially unlimited cell source and promising patient-specific in vitro model of cardiac diseases. Yet, these cells are limited by immaturity and population heterogeneity. Current in vitro studies aiming at better understanding of the
Maosheng Zhang et al.
Talanta, 81(1-2), 162-166 (2010-03-02)
A novel protein molecularly imprinted membrane (PMIM) was synthesized on the surface of multiwalled carbon nanotubes (MWNTs) through a surface molecular imprinting technique by using bovine serum albumin (BSA) as the template molecule, acrylamide (AAm) as the functional monomer, N,N'-methylenebisacrylamide
Hyunwoo Yuk et al.
Nature communications, 8, 14230-14230 (2017-02-02)
Sea animals such as leptocephali develop tissues and organs composed of active transparent hydrogels to achieve agile motions and natural camouflage in water. Hydrogel-based actuators that can imitate the capabilities of leptocephali will enable new applications in diverse fields. However
Chun-Ping Liu et al.
International journal of molecular sciences, 16(6), 13692-13713 (2015-06-18)
Manchurian ash (Fraxinus mandshurica Rupr.) is a valuable hardwood species in Northeast China. In cultures of F. mandshurica, somatic embryos were produced mainly on browned explants. Therefore, we studied the mechanism of explant browning and its relationship with somatic embryogenesis
Jeonghee Yeom et al.
Science advances, 6(23), eaba5785-eaba5785 (2020-06-18)
Artificial tongues have been receiving increasing attention for the perception of five basic tastes. However, it is still challenging to fully mimic human tongue-like performance for tastes such as astringency. Mimicking the mechanism of astringency perception on the human tongue
Varsha Singh et al.
American journal of physiology. Gastrointestinal and liver physiology, 307(6), G623-G631 (2014-07-26)
Probiotics, including Lactobacilli, are commensal bacteria that have been used in clinical trials and experimental models for the prevention and treatment of diarrheal disorders. Our previous studies have shown that Lactobacillus acidophilus (LA) and its culture supernatant (CS) stimulated Cl(-)/HCO3
Tomáš Ječmen et al.
Methods (San Diego, Calif.), 89, 128-137 (2015-08-04)
Protein-protein interactions play a central role in the regulation of many biochemical processes (e.g. the system participating in enzyme catalysis). Therefore, a deeper understanding of protein-protein interactions may contribute to the elucidation of many biologically important mechanisms. For this purpose
Kelsey I MacConaghy et al.
The Analyst, 140(18), 6354-6362 (2015-08-14)
We have developed a novel approach for DNA detection as well as genetic screening of mutations by uniquely combining DNA-responsive and optically diffracting materials. This approach entails the polymerization of a photonic crystal within a hydrogel network that alters the
Zifu Li et al.
Langmuir : the ACS journal of surfaces and colloids, 31(23), 6282-6288 (2015-04-04)
Two distinct uniform hybrid particles, with similar hydrodynamic diameters and comparable zeta potentials, were prepared by copolymerizing N-isopropylacrylamide (NIPAM) and styrene. These particles differed in their styrene to NIPAM (S/N) mass ratios of 1 and 8 and are referred to
Xiaojia Huang et al.
Journal of separation science, 34(23), 3418-3425 (2011-11-05)
In this study, a new stir bar sorptive extraction (SBSE) coating based on poly(vinylphthalimide-co-N,N'-methylenebisacrylamide) monolith (SBSE-VPMB) was prepared. The influences of the contents of monomer in polymerization mixture and the percentage of porogen solvent on the extraction performance were investigated
Degang Wang et al.
Molecular medicine reports, 11(1), 175-181 (2014-10-24)
Preparations utilizing monoclonal antibodies against S100A4 provide useful tools for functional studies to investigate the clinical applications of the human S100A4 protein. In the present study, human S100A4 protein was expressed in Escherichia coli (E. coli) BL21 (DE3), successfully purified
Takaki Amamoto et al.
Chemical & pharmaceutical bulletin, 62(7), 649-653 (2014-07-06)
We have developed a general method for controlling molecular functions using a photodegradable hydrogel; gels containing molecules made from such materials are capable of release and activation by light stimulus. As the elimination of molecular leaching from the gel before
Qingsi Li et al.
Journal of colloid and interface science, 555, 145-156 (2019-08-05)
Resin hemoperfusion is a life-saving treatment for drug intoxication or hepatic failure of patients. However, current resin adsorbents exhibit a limited hemocompatibility or low adsorption efficiency, representing a major roadblock to successful clinical applications. In this work, we developed a
Marcio H M da Luz et al.
Journal of neurochemistry, 153(3), 377-389 (2020-01-18)
PrPC is a glycoprotein capable to interact with several molecules and mediates diverse signaling pathways. Among numerous ligands, laminin (LN) is known to promote neurite outgrowth and memory consolidation, while amyloid-beta oligomers (Aβo) trigger synaptic dysfunction. In both pathways, mGluR1
Ewa Moczko et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1124, 1-6 (2019-06-09)
Herein an approach to prepare molecularly imprinted polymer nanoparticles (nanoMIPs) with specific binding affinity for antibodies is reported. The process relied on the covalent immobilization of the template (whole immunoglobulin G (IgG), Fc domain of human IgG and peptide epitope)
Jo Sing Julia Tang et al.
Molecules (Basel, Switzerland), 24(10) (2019-05-18)
Interactions between glycans and proteins have tremendous impact in biomolecular interactions. They are important for cell-cell interactions, proliferation and much more. Here, we emphasize the glycan-mediated interactions between pathogens and host cells. Pseudomonas aeruginosa, responsible for a huge number of
Avisek Mahapa et al.
PloS one, 10(3), e0122168-e0122168 (2015-03-31)
SarA, a Staphylococcus aureus-specific dimeric protein, modulates the expression of numerous proteins including various virulence factors. Interestingly, S. aureus synthesizes multiple SarA paralogs seemingly for optimizing the expression of its virulence factors. To understand the domain structure/flexibility and the folding/unfolding
Alok K Shah et al.
Molecular & cellular proteomics : MCP, 14(11), 3023-3039 (2015-09-26)
We report an integrated pipeline for efficient serum glycoprotein biomarker candidate discovery and qualification that may be used to facilitate cancer diagnosis and management. The discovery phase used semi-automated lectin magnetic bead array (LeMBA)-coupled tandem mass spectrometry with a dedicated
Sora Yun et al.
Acta biochimica et biophysica Sinica, 47(11), 938-945 (2015-09-18)
The TetR family transcriptional regulator AefR contributes to the regulation of the quorum-sensing system. However, the role of AefR in the regulatory network of the phytopathogen Pseudomonas syringae pathovars is not known. In this study, the phenotype of a P.
Pierre Grégoire Guinot et al.
European journal of anaesthesiology, 32(8), 535-542 (2014-09-06)
Several authors have suggested that right ventricular dysfunction (RVd) may contribute to renal dysfunction in nonsurgical patients. We tested the hypothesis that RVd diagnosed immediately after cardiac surgery may be associated with subsequent development of renal dysfunction and tried to
Sebastian Seiffert et al.
Journal of colloid and interface science, 452, 38-42 (2015-04-29)
Microfluidic droplet templating produces monodisperse particles of well controllable sizes, but this is limited by the necessity to operate microfluidic devices at low flow rates in the dripping regime. Here, the per-channel rate of droplet production could be substantially increased
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