156868

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A simplified synthesis of polymeric nonparticulate stationary phases with macrocyclic antibiotic as chiral selector for capillary electrochromatography.

Olga Kornysova et al.

Electrophoresis, 25(16), 2825-2829 (2004-09-08)

A simplified approach to synthesize nonparticulate (continuous or monolithic) beds with embedded vancomycin chiral selectors for capillary electrochromatography is proposed. In the present approach, N,N'-diallyltartardiamide monomer with diol functionality is used, which can be readily converted to aldehyde groups via

Development of a metal/chelate polyhydroxyethylmethacrylate monolith capillary for selective depletion of immunoglobulin G from human plasma for proteomics.

Ashish Khaparde et al.

Journal of chromatography. A, 1517, 117-125 (2017-09-02)

In this study, we report the development of a new poly HEMA (HEMA-co-DEGDA-co-DATD) monolith capillary functionalized with "IDA-Cu (II) complex". Of the two tested crosslinkers (methylene bisacrylamide (MBAAm) and diethylene glycol diacrylate (DEGDA)), presence of DEGDA has enhanced the monolith

Journal of Chromatography A, 638, 165-165 (1993)

Treatment with Bumped Kinase Inhibitor 1294 Is Safe and Leads to Significant Protection against Abortion and Vertical Transmission in Sheep Experimentally Infected with Toxoplasma gondii during Pregnancy.

Roberto Sánchez-Sánchez et al.

Antimicrobial agents and chemotherapy, 63(7) (2019-05-08)

Previous studies on drug efficacy showed low protection against abortion and vertical transmission of Toxoplasma gondii in pregnant sheep. Bumped kinase inhibitors (BKIs), which are ATP-competitive inhibitors of calcium-dependent protein kinase 1 (CDPK1), were shown to be highly efficacious against

Simplification of affinity macroporous monolith microfluidic column synthesis and its ability for protein separation.

Ashish Khaparde et al.

Journal of pharmaceutical and biomedical analysis, 181, 113099-113099 (2020-01-25)

A generic multi-component approach was designed to perform simultaneous in situ polymerization and ligand immobilization to develop affinity porous polymer based chromatography resin in a facile mode. This strategy exploits the regioselective ring opening reaction between epoxy group of monomer

Investigating the reaction of a number of gel electrophoresis cross-linkers with beta-lactoglobulin by matrix assisted laser desorption/ionization-mass spectrometry.

M Galvani et al.

Electrophoresis, 21(17), 3684-3692 (2001-03-29)

A number of cross-linkers that are commonly used in polyacrylamide gels have been incubated with bovine beta-lactoglobulin B and the resulting reaction mixtures were examined by matrix assisted laser desorption/ionization-mass spectrometry. At concentrations of 0.1, 1, and 20 mM of

Parallel Induction of CH505 B Cell Ontogeny-Guided Neutralizing Antibodies and tHIVconsvX Conserved Mosaic-Specific T Cells against HIV-1.

Edmund G Wee et al.

Molecular therapy. Methods & clinical development, 14, 148-160 (2019-08-02)

The aim of this work was to start collecting information on rational combination of antibody (Ab) and T cell vaccines into single regimens. Two promising candidate HIV-1 vaccine strategies, sequential isolates of CH505 virus Envs developed for initiation of broadly neutralizing

Smart valve: Polymer actuator to moisture soil control

Romero MR, et al.

Sensors and Actuators B, Chemical, 234(2), 53-62 (2016)

Using 4-vinylcyclohexene diepoxide as a model of menopause for cardiovascular disease.

John P Konhilas et al.

American journal of physiology. Heart and circulatory physiology, 318(6), H1461-H1473 (2020-05-10)

There is a sharp rise in cardiovascular disease (CVD) risk and progression with the onset of menopause. The 4-vinylcyclohexene diepoxide (VCD) model of menopause recapitulates the natural, physiological transition through perimenopause to menopause. We hypothesized that menopausal female mice were

Information on DNA conformation derived from the Ferguson plot of DNA fragments of up to 9 kb in size, using polyacrylamide gel electrophoresis in a discontinuous buffer system.

L Orbán et al.

Electrophoresis, 12(4), 241-246 (1991-04-01)

The Ferguson plot in polyacrylamide gel electrophoresis (PAGE)(15%CDATD, moving boundary electrophoresis buffer system operative at pH 8.9, 4 degrees C, 8 mA/cm2 of gel) of DNA fragments up to 9.4 kb in size was found to exhibit a linear segment

Towards new formulations for polyacrylamide matrices, as investigated by capillary zone electrophoresis

Righetti PG, et al.

Journal of Chromatography A, 638(2), 165-178 (1993)

N-N' diallyltartardiamide (DATD) as a cross-linking agent for polyacrylamide gel disc electrophoresis of human serum proteins.

R S Kelkar et al.

Journal of postgraduate medicine, 32(1), 27-31 (1986-01-01)

Membrane protein pattern in hereditary spherocytosis in five subjects from north-east Italy obtained by SDS-PAGE using N,N'-diallyltartardiamide.

A L Giuliani et al.

European journal of haematology, 63(5), 302-305 (1999-12-02)

In the present study we examined five subjects affected by hereditary spherocytosis (three unsplenectomized and two splenectomized), coming from an area in the north-east of Italy where hereditary spherocytosis is an anaemic disease with very low incidence. All patients showed

Liquid scintillation counting of polyacrylamide gels crosslinked with N,N'-methylene-bis-acrylamide and N,N'-diallyltartardiamide.

P A Moore

Analytical biochemistry, 108(1), 151-155 (1980-10-01)

A novel gel based on an ionic complex from a dendronized polymer and ciprofloxacin: Evaluation of its use for controlled topical drug release

Garcia MC, et al.

Materials Science and Engineering, C, 69(2), 236-246 (2016)

Electrophoresis and electrofocusing on polyacrylamide gel in the study of native macromolecules.

A Chrambach

Molecular and cellular biochemistry, 29(1), 23-46 (1980-01-16)

In situ genome sequencing resolves DNA sequence and structure in intact biological samples.

Andrew C Payne et al.

Science (New York, N.Y.), 371(6532) (2021-01-02)

Understanding genome organization requires integration of DNA sequence and three-dimensional spatial context; however, existing genome-wide methods lack either base pair sequence resolution or direct spatial localization. Here, we describe in situ genome sequencing (IGS), a method for simultaneously sequencing and

A method for quantifying radioactivity associated with protein in silver-stained polyacrylamide gels.

W R Springer

Analytical biochemistry, 195(1), 172-176 (1991-05-15)

A method is described in which individual proteins labeled with weak beta-emitting radionuclides, separated by polyacrylamide gel electrophoresis, and stained with silver are released from the gel by the use of the periodate soluble cross-linking agent N,N'-dialyltartardiamide. The radioactivity can

Quantitative measurement of protein mass and radioactivity in N,N'-diallyltartardiamide crosslinked polyacrylamide slab gels.

R B Young et al.

Analytical biochemistry, 108(1), 202-206 (1980-10-01)

Post-synthesis modification of hydrogels. Total and partial rupture of crosslinks: Formation of aldehyde groups and re-crosslinking of cleaved hydrogels.

Wolfel A, et al.

Polymer (2017)

Properties of acrylamide gels cross-linked with low concentrations of N,N'-diallyltartardiamide.

E C Hahn et al.

Journal of virological methods, 15(1), 41-52 (1987-01-01)

The behavior of proteins on acrylamide gels cross-linked with low concentrations of N,N'-diallyltartardiamide (DATD) has been studied from a theoretical and practical point of view. Modifications in methodology and a technique for calculation of apparent molecular weights (MWs) allowed accurate

A micro-solid phase extraction device to prepare a molecularly imprinted porous monolith in a facile mode for fast protein separation.

Riddhi Mehta et al.

Journal of chromatography. A, 1627, 461415-461415 (2020-08-22)

A molecularly imprinted polymeric monolith was synthesized in an aqueous environment in 15 min via UV-irradiation. The imprinted monolith was composed of hydroxyethyl methacrylate as monomer, dimethyl amino ethyl methacrylate as functional monomer, methylene bisacrylamide and piperazine diacrylamide as crosslinkers and

Novel poly (NIPA-co-AAc) functional hydrogels with potential application in drug controlled release.

Cuggino JC, et al.

Molecular Pharmaceutics, 11(7), 2239-2249 (2014)

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