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显示 1-30 共 56 条结果 关于 "19123" 范围 论文
Ruimin Sun et al.
British journal of pharmacology, 177(18), 4166-4180 (2020-06-11)
Hepatic fatty acid metabolism disorder, a key pathogenic mechanism underlying non-alcoholic fatty liver disease (NAFLD), is associated with the hyperacetylation of mitochondrial enzymes. Acyl-CoA synthetase family member 3 (ACSF3), which is involved in the regulation of fatty acid metabolism, was
Fernando Ezquer et al.
PloS one, 6(1), e16566-e16566 (2011-02-10)
Type 1 diabetes mellitus (T1D) is due to autoimmune destruction of pancreatic beta-cells. Previously, we have shown that intravenously administered bone marrow-derived multipotent mesenchymal stromal cells (MSCs) allows pancreatic islet recovery, improves insulin secretion and reverts hyperglycemia in low doses
Nausika Betriu et al.
Biomolecules, 10(5) (2020-05-02)
Hair follicle dermal papilla cells (HFDPC) are a specialized cell population located in the bulge of the hair follicle with unique characteristics such as aggregative behavior and the ability to induce new hair follicle formation. However, when expanded in conventional
J P Neves Silva et al.
Biochimica et biophysica acta, 1838(10), 2555-2567 (2014-07-01)
Dioctadecyldimethylammonium bromide (DODAB):Monoolein (MO) lipoplexes have mainly been studied within the range of high molar ratios of DODAB, with noticeable transfection efficiencies in the Human Embryonic Kidney (HEK, a.k.a. 293T) cell line. In this work, we intend to study the
Albert Herms et al.
Nature communications, 6, 7176-7176 (2015-05-28)
Lipid droplets (LDs) are intracellular organelles that provide fatty acids (FAs) to cellular processes including synthesis of membranes and production of metabolic energy. While known to move bidirectionally along microtubules (MTs), the role of LD motion and whether it facilitates
Ulf Maeder et al.
Journal of biomedical optics, 17(10), 106011-106011 (2012-12-12)
A confocal imaging and image processing scheme is introduced to visualize and evaluate the spatial distribution of spectral information in tissue. The image data are recorded using a confocal laser-scanning microscope equipped with a detection unit that provides high spectral
Yaroslav V Faletrov et al.
The FEBS journal, 280(13), 3109-3119 (2013-04-05)
The fluorescent probes Nile Red (nonsteroidal dye) and 25-{N-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-methyl]amino}-27-norcholesterol (25-NBD-cholesterol) (a cholesterol analog) were evaluated as novel substrates for steroid-converting oxidoreductases. Docking simulations with autodock showed that Nile Red fits well into the substrate-binding site of cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17A1)
Ruiying Song et al.
The journal of physical chemistry. B, 119(9), 3762-3767 (2015-02-17)
In this paper, we report a micelle-to-vesicle transition in aqueous solution of the anionic single-tailed surfactant (STS) sodium dodecylbenzenesulfonate (SDBS), with the mediation of a rough glass surface (RGS) in the absence of cosurfactants or additives. This transition produced a
Feyza Gunduz et al.
Virology journal, 9, 143-143 (2012-08-07)
Hepatic steatosis is recognized as a major risk factor for liver disease progression and impaired response to interferon based therapy in chronic hepatitis C (CHC) patients. The mechanism of response to interferon-alpha (IFN-α) therapy under the condition of hepatic steatosis
Michitaka Suzuki et al.
Methods in molecular biology (Clifton, N.J.), 931, 483-491 (2012-10-03)
Cells store excess lipid as esters in the form of triglycerides and cholesterol esters. Most lipid esters are compartmentalized in globular structures called lipid droplets. Here we describe several methods of detecting lipid droplets by fluorescence microscopy. Lipid droplets can
Karel A Erion et al.
The Journal of biological chemistry, 290(26), 16191-16201 (2015-05-03)
Hyperinsulinemia (HI) is elevated plasma insulin at basal glucose. Impaired glucose tolerance is associated with HI, although the exact cause and effect relationship remains poorly defined. We tested the hypothesis that HI can result from an intrinsic response of the
Robert D Gardner et al.
Biotechnology and bioengineering, 110(1), 87-96 (2012-07-07)
Microalgae are capable of accumulating high levels of lipids and starch as carbon storage compounds. Investigation into the metabolic activities involved in the synthesis of these compounds has escalated since these compounds can be used as precursors for food and
Manuel Quiñones-Pérez et al.
Acta biomaterialia, 121, 339-348 (2020-12-04)
Silicones (i.e. crosslinked poly(dimethylsiloxane), PDMS) are commonly used material for microfluidic device fabrication. Nonetheless, due to the uncontrollable absorption of small hydrophobic molecules (<1 kDa) into the bulk, its applicability to cell-based drug assays and sensing applications has been limited. Here
Kazuhito Tanabe et al.
Bioorganic & medicinal chemistry letters, 22(23), 7045-7047 (2012-10-23)
We have designed a novel aggregate of DNA block copolymer (DBC) that is sensitive to hypoxic X-irradiation. The DBC consists of tetrahydropyrane-protected 2-hydroxyethyl methacrylate as a hydrophobic unit and oligodeoxynucleotides as a hydrophilic unit, which are linked to a radiation-sensitive
Centdrika R Dates et al.
Cancer biology & therapy, 16(5), 714-723 (2015-05-23)
Increased aerobic glycolysis and de novo lipid biosynthesis are common characteristics of invasive cancers. UDP-glucuronosyltransferases (UGTs) are phase II drug metabolizing enzymes that in normal cells possess the ability to glucuronidate these lipids and speed their excretion; however, de-regulation of
Claire Levrier et al.
Journal of natural products, 78(1), 111-119 (2015-01-13)
Eight new dihydro-β-agarofurans, denhaminols A-H (1-8), were isolated from the leaves of the Australian rainforest tree Denhamia celastroides. The chemical structures of 1-8 were elucidated following analysis of 1D/2D NMR and MS data. The absolute configuration of denhaminol A (1)
Nobuo Uehara et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 28(12), 1125-1132 (2012-12-13)
Thermoresponsive gold nanocomposites encapsulating Nile red were fabricated by the conjugation of gold nanoparticles containing Nile red with a thermoresponsive polymer, poly(N-isopropylacrylamide(90 mol%)-co-N-acryloyldiethylenetriamine(10 mol%)). They were then examined as a model of drug delivery carriers and colloidal fluorescence sensors. Nile
Guo-Dong Feng et al.
Bioresource technology, 128, 107-112 (2012-12-01)
To characterize lipid content of microalgal cells rapidly and accurately, the gravimetric determination, FT-IR and Nile Red (NR) staining were investigated on six typical eukaryotic and prokaryotic algae species. FT-IR and Nile Red were relative quantification methods and a standard
Mehrnaz Azami-Movahed et al.
The international journal of biochemistry & cell biology, 45(2), 299-307 (2012-10-24)
A number of ligands with affinities for the heme binding site of apomyoglobin were tested to control amorphous and fibrillar aggregation in the protein. Several techniques, including fluorescence, dynamic light scattering, transmission electron microscopy, dot blot analysis combined with viability
Minnie Chan et al.
Analytical chemistry, 84(18), 7779-7784 (2012-08-16)
A new method to precisely monitor rapid release kinetics from polymeric particles using super paramagnetic iron oxide nanoparticles, specifically by measuring spin-spin relaxation time (T(2)), is reported. Previously, we have published the formulation of logic gate particles from an acid-sensitive
Nile red: a selective fluorescent stain for intracellular lipid droplets.
Greenspan P, et al.
The Journal of Cell Biology, 100(3), 965-973 (1985)
Zhen Zhang et al.
Biomaterials, 33(29), 7233-7240 (2012-07-17)
Besides as an inert carrier for hydrophobic anticancer agents, polymeric micelles composed of di-block copolymer poly(ethylene glycol)-poly(lactic acid) (PEG-b-PLA) function as biological response modifiers including reversal of multidrug resistance in cancer. However, the uptake mechanisms and the subsequent intracellular trafficking
Daisuke Horita et al.
Chemical & pharmaceutical bulletin, 62(6), 578-585 (2014-06-03)
The hair follicle-plugging method was used to analyze the effects of EtOH on skin permeation pathways. In vitro permeation experiments were performed on 4 model drugs [isosorbide mononitrate (ISMN), ionized lidocaine (ionized LC), fluorescein (FL), and fluorescein isothiocyanate (FITC)-dextran 4
Ryan B Smit et al.
PLoS genetics, 4(10), e1000222-e1000222 (2008-10-18)
The Caenorhabditis elegans pharynx (or foregut) functions as a pump that draws in food (bacteria) from the environment. While the "organ identity factor" PHA-4 is critical for formation of the C. elegans pharynx as a whole, little is known about
Ni Zeng et al.
International journal of nanomedicine, 7, 3703-3718 (2012-08-14)
Lipid-based liquid crystalline nanoparticles (LCNPs) have attracted growing interest as novel drug-delivery systems for improving the bioavailability of both hydrophilic and hydrophobic drugs. However, their cellular interaction and in vivo behavior have not been fully developed and characterized. In this
James A Levitt et al.
Journal of biomedical optics, 20(9), 096002-096002 (2015-09-04)
Spectrally resolved confocal microscopy and fluorescence lifetime imaging have been used to measure the polarity of lipid-rich regions in living HeLa cells stained with Nile red. The emission peak from the solvatochromic dye in lipid droplets is at a shorter
Wantong Song et al.
Macromolecular bioscience, 12(10), 1375-1383 (2012-08-29)
The pH sensitivity of a series of PbAEs synthesized from primary amines and diacrylates is studied. By changing alkyl groups of the amine monomers, the pKb can be tuned across a broad range (from 3.5 to 7.2). Micelles formed from
Kevin B Vargo et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(29), 11657-11662 (2012-07-04)
Using recombinant amphiphilic proteins to self-assemble suprastructures would allow precise control over surfactant chemistry and the facile incorporation of biological functionality. We used cryo-TEM to confirm self-assembled structures from recombinantly produced mutants of the naturally occurring sunflower protein, oleosin. We
Wei-Chih Chao et al.
Biochemistry, 52(6), 1113-1121 (2013-01-19)
Various fluorescence experiments and computer simulations were utilized to gain further understanding of thromboxane A(2) synthase (TXAS), which catalyzes an isomerization of prostaglandins H(2) to give rise to thromboxane A(2) along with a fragmentation reaction to 12-L-hydroxy-5,8,10-heptadecatrienoic acid and malondialdehyde.
Cristian Vilos et al.
Biomaterials, 34(16), 4098-4108 (2013-03-08)
This report is an integrated study to include the molecular simulation, physicochemical characterization and biological analysis of a paclitaxel-loaded PHBV nanoparticle that demonstrates uptake, release and cytotoxicity in cancer cell lines. Taking this nanoparticle one step closer to its use
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