• Boiling sulfuric acid
• Fluorine gas at 200°C (392°F)
• Chlorine trifluoride at 10–50°C (50–122°F)
• Uranium hexafluoride gas at 50°C (122°F)
• Molten sodium or potassium hydroxide at
voltage 3 kV, Fragmentor
150 V, Mass range 50-200 m/z, Split to MS 100 µL/min; Injection: 5 µL in
mobile phase Ref. [3].
Separation of Cations
Figure 6. Separation of tryptic digests of bovine serum
Chimpanzee and gorilla
the immunogen sequence is 100% identical. Orangutan (130/132), pig (122/132), and dog
(122/132).
FORMAT: Affinity purified immunoglobulin.
PRESENTATION: Lyophilized from
200
References
1. Kolb, et al., J. Immunol., 122, 2103 (1979)
2. Kabat, E.A. and Mayer, M.M., Experimental
Immunochemistry, Springfield, IL, Charles C.
Thomas, 2nd edition, 149 (1961)
4/99
Res. 122:582-585 (1977).
3. Ahlman, et al., Int. J. Cancer 43:949-955 (1989).
4. Wang, et al., J. Neuro Methods 33:219-227 (1990).
5. Bongarzone et al., J. Neuroscience 18:5344-5353 (1998).
6. Gau
EDTA and 134 mM
KCl.
9
Choline oxidase solutions may also be prepared
in 200 mM Trizma
®
-HCl, pH 8.0.
6
Another publication
cites preparation of 2 mg/mL stock solutions of choline
Letters 317:119-122.
Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product
vial. For products with volumes of 200 µL or less
Solution* Molybdenum Reagent
Working Solution
Test 10 µl 200 µl
Blank – 200 µl
Standard 10 µl 200 µl
* Added in order to stop the reaction
6. Add 10 µl of SDS 10% solution (Product Code
methylamine)
0.1 M
0.5 M
1.0 M
4.0 M
122
106
90
86
Guanidine hydrochloride 0.1 M
0.5 M
1.0 M
60
27
12
Acetonitrile 1% (v/v)
5% (v/v)
10% (v/v)
122
157
161
of stated concentrations of
denaturing agents. Incubation of Endoproteinase Asp-N 200 μg/ml with denaturing agent for 6 hours at +25°C
in 25 mM sodium phosphate buffer, pH 7.8.
Add 20 mM methylamine
glycerol.
Molecular mass: ∼66 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 122-166 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not
648463 647.0 (avg.) Non-ionic 157
TWEEN® 20, PROTEIN GRADE® Detergent 655206 1228.0 (avg.) Non-ionic 122
TRITON® is a registered trademark of Rohm and Haas; TWEEN® is a registered trademark of ICI.