200-302-4 | Sigma-Aldrich

EX-CELL 302 Serum-Free Medium for CHO Cells

cold EX-CELLTM 302 medium. 3. Using low-speed centrifugation, pellet the cell suspension at 200 g for 5 minutes and carefully decant the supernatant without disturbing the cell pellet. 4. Resuspend the

EX-CELL 302 Serum-Free Medium for CHO Cells

cold EX-CELLTM 302 medium. 3. Using low-speed centrifugation, pellet the cell suspension at 200 g for 5 minutes and carefully decant the supernatant without disturbing the cell pellet. 4. Resuspend the

Product Information Sheet - 14326C

EX-CELL® 302 is formulated with sodium bicarbonate and without L-glutamine. For applications requiring the use of L-glutamine, supplement with 4 mM L-glutamine by adding 20 mL/L of a 200 mM solution

Product Information Sheet - 14324C

EX-CELL® 302 is formulated with sodium bicarbonate and without L-glutamine. For applications requiring the use of L-glutamine, supplement with 4 mM L-glutamine by adding 20 mL/L of a 200 mM solution

Animal-Component Free Recombinant Human Insulin is Suitable for Use in Serum-Free Media

use, all media were supplemented with the following concentrations of L-glutamine: • EX-CELLTM 302 — 4 mM • EX-CELLTM 293 — 6 mM • EX-CELLTM VPRO — 6 mM All cultures were seeded at 3 x105 cells/mL, in

EX-CELL Serum-Free Media for CHO Cells

selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELLTM 302 was developed using only recombinant human

EX-CELL 325 PF CHOSerum-Free Medium for CHO Cells Protein-Free

L-glutamine. For applications requiring the use of L-glutamine, supplement with 4 mM L-glutamine prior to use by adding 20 mL/L of a 200 mM solution (Catalog No. 59202C). SAFC Biosciences recommends L-glutamine

EX-CELL 325 PF CHO Serum-Free Medium for CHO Cells

gas. NOTE: For applications requiring the use of L-glutamine, supplement with 4 mM L-glutamine by adding 20 mL/L of a 200 mM solution (Catalog No. 59202C) prior to use. SAFC Biosciences recommends L-glutamine

GelRed® Nucleic Acid Stain (10,000X, Water)

Cast the gel and allow it to solidify. 4. Load samples and run the gels using your standard protocol. 5. View the stained gel using a standard transilluminator (302 or 312 nm) and image the gel using an

GelRed® Nucleic Acid Stain

precast gels at 4°C can cause dye precipitation and poor performance. 4. Load samples and run the gels using your standard protocol. 5. View the stained gel with a standard transilluminator (302 or 312 nm)

GelRed® Nucleic Acid Stain (10,000X, DMSO)

Cast the gel and allow it to solidify. 4. Load samples and run the gels using your standard protocol. 5. View the stained gel using a standard transilluminator (302 or 312 nm) and image the gel using an

GelRed® Nucleic Acid Stain (3X, Water)

precast gels at 4°C can cause dye precipitation and poor performance. 4. Load samples and run the gels using your standard protocol. 5. View the stained gel with a standard transilluminator (302 or 312 nm)

4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b)

Save the supernatant for application. Control Suggested Positive Antigen Control: Cat.# 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting

Development and Application of a Chemically Defined Bioreactor Feed for CHO Cells

11397 Media and Reagents • EX-CELL 302 Serum-Free Medium for CHO Cells, SAFC, Catalog No. 14324C • EX-CELL 66131, SAFC, Product No. 66131C • L-Glutamine, 200 mM solution, Sigma-Aldrich, Catalog

Data Sheet - A0310

and Cline HT., Trends Neurosci., 30, 382-9 (2007). 10. Nigam R., et al., Arch Dermatol Res., 302, 507-15 (2010). 11. Watanabe M., et al., Int Rev Cytol., 213, 1-47 (2002). 12. Ring H.

Data Sheet - P5247

6. Pei, L., Proc. Natl. Acad. Sci. USA, 100, 7803-7807 (2003). 7. Mu, D., Cancer Cell, 3, 297-302 (2003). 8. Lauritzen, I. et al., J. Biol. Chem., 278, 32068- 32076(2003) MCT/PHC 08/04

Data Sheet - MSQC13

Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com. SILu is a trademark

Product Information - Z273546

) –1.0 x 10–13 Torr at 200°C (392°F) –1.0 x 10–5 kPa at 20°C (68°F) –1.0 x 10–14 kPa at 200°C (392°F) –1.0 x 10–6 Evaporation Loss 4 x 10–6 torr at 150°C

Data Sheet - MSQC11

Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881- 9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com. SILu is a trademark of

MSQC29 - Datasheet

Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877- 881- 9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com. SILu is a trademark of

Lck KinEASE™ FP-645nm FarRed Assay

combine 510 µL water, 280 µL 0.5M EDTA pH 7.2, 200 µL 5X Detection Buffer and 10 µL 100X TK Tracer-red). 5 µL of TK Stop Mix is required per well. 4. TK Antibody Mix:

MSQC25 - Datasheet

Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877- 881- 9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com. SILu is a trademark of

Data Sheet - P1106

This is particularly striking considering the nervous system of C. elegans is comprised of only 302 neurons. In mammals, 9 different 4TM/2P K+ channels, designated as the KCNK family, have been cloned

Data Sheet - MSST0003

Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881- 9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com. KR,NA,RC,MAM 08/16

Data Sheet - P0981

most diverse group of K+ channels in this organism.4 This is particularly striking considering the nervous system of C. elegans is comprised of only 302 neurons. In mammals, 9 different 4TM/2P K+ channels

KiCqStart SYBR Green Primers Gene Arrays Safety Data Sheet

CAS-No. EC-No. 56-81-5 200-289-5 >= 10 - 200-664-3 Flam. Liq. 4; H227 >= 10 - < 20 % For the full

Data Sheet - MSQC21

Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877- 881- 9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com. SILu is a trademark of

Data Sheet - MSST0019

Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881- 9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com. KR,AA,NA,AI,MAM 08/

Data Sheet - MSST0015

Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881- 9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com. KR,MAM 02/17-1

Data Sheet - MSQC19

Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881- 9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com. SILu is a trademark of