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h on ice.
7. Centrifuge cells at 200 x g for 5 min. Remove supernatant. Resuspend
pellet in 2.5 ml Cold Efflux Buffer per 106 cells.
8. Repeat step 7.
9. At this point
7.4, containing 50% glycerol for a final volume of 300
µL.
Anti-Rac1, clone 23A8, Catalog # 05-389, see page two for
more information. One vial containing 250 µg of protein G
purified mouse IgG2b
RT-PCR Kit
sigma-aldrich.com 9
3. Results
Fig. 2: High sensitivity for difficult templates. RT-PCR was performed on a dilution series of the HeLa RNA. A
389 bp fragment was amplified with primers