based on the
activated autoxidation of a tetracyclic catechol.
Anal. Biochem., 214(2), 442-451 (1993).
5. Sigrist, M., and Staehelin, L. A., Identification of
type 1 and type 2 light-harvesting chlorophyll
Finkelstein, L.D. et al., Tec kinases: shaping T-cell
activation through actin. Trends Cell Biol., 14, 443-
451 (2004).
PRECISIO is a registered trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich
assay.
References
1. Herman, I. M., Curr. Opin. Cell Biol., 5, 48 (1993).
2. Vandekerckhove, J., and Weber, K., Eur. J. Bio-
chem., 90, 451 (1978).
2012 Sigma-Aldrich Co. LLC. All rights
4. If the plate cannot be read immediately, add 50 L
of 3 N NaOH solution per 200 L of reaction
mixture.
5. Read the absorbance for the stopped reactions at
405 nm.
Finkelstein, L.D. et al., Tec kinases: shaping T-cell
activation through actin. Trends Cell Biol., 14, 443-
451 (2004).
TLD,MAM 02/09-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
Finkelstein, L.D. et al., Tec kinases: shaping T-cell
activation through actin. Trends Cell Biol., 14, 443-
451 (2004).
TLD,MAM 04/09-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
far.
IMMUNOGEN: Purified peptide from amino acids 451-467 of human Kv4.3 (Accession number O60577).
APPLICATIONS: Western blot: 1:200 using ECL on rat brain membranes.
Immunohistochemistry
is comparatively insoluble in water, but is quite soluble in base. (Saturation is
approximately 200 mg/mL.5 A4685, the sodium salt, is readily soluble in water.)1 Solutions are very sensitive
to light and
CDK8) is developed in
rabbit using a synthetic peptide corresponding to the
C-terminal region (aa 451-464) of human CDK8. The
antibody is purified by protein A affinity chromatography.
Anti-CDK8 specifically
2000).
3. Bird, A., and Wolffe, A.P., Cell, 99, 451-454
(1999).
4. Razin, A., and Szyf, M., Biochim. Biophys.
Acta, 782, 331-342 (1984).
5. Nur, I., et al., Nuc. Acids Res., 16
Weber, K., Eur. J.
Biochem., 90, 451 (1978).
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(1991).
4. Lessard, J., Cell Motil. Cytoskel., 10, 349 (1988).
5. Sutoh, K., and Mabuchi, I., Biochemistry
D., J. Struct. Biol., 129,
102-122 (2000).
4. Bird, A., and Wolffe, A.P., Cell, 99, 451-454
(1999).
5. Sudarsanam, P., and Winston, F., TIG, 16,
345-350 (2000).
6. Wang, W., et al., EMBO
of the acute leukemias. French-American-British (FAB)
Co-operative Group. Brit J Haematol 33:451, 1976.
5. Marmont AM, Damasio E, Zucker-Franklin D: Neutrophils. In Atlas of Blood Cells-
Function and
, The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
PRECISIO is a registered trademark of Sigma-Aldrich
Co. LLC.
JR,JB,MAM 05/12-1
, The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
Precisio is a trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich Co.
, The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
Precisio is a trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich Co.
, The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
TLD,MAM 02/09-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
, The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
Precisio is a registered trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich
, The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
TLD,MAM 04/09-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
, The Aurora kinases: role in cell
transformation and tumorigenesis. Cancer
Metastasis Rev., 22, 451-464 (2003).
TLD,MAM 02/09-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich