expressed in CHO cells).
Figure 5. Rate of inactivation the hKv3.3 channel.
Typical current trace of hKv3.3 channel stepped to +30 mV from a holding potential of –80
mV for 200 ms. Time course of inactivation
Microwave irradiation Place the slide(s) in a plastic jar containing 200 mL 0.1 M citrate buffer, pH 6.0.
Apply 350 W microwave irradiation for 5 minutes.
Rinse slide(s) twice with PBS.
Microwave irradiation Place the slide(s) in a plastic jar containing 200 ml 0.1 M citrate buffer, pH 6.0.
Apply 350 W microwave irradiation for 5 minutes.
Rinse slide(s) twice with PBS.
Sampling Flow Rate From 20 mL/min –
850 mL/min
From 20 mL/min –
200 mL/min
Sampling Time From 5 minutes up to
12 hrs.
From 5 minutes up to
4 hrs.
Storage Ambient (before and
after
Microwave irradiation Place the slide(s) in a plastic jar containing 200 ml 0.1 M citrate buffer, pH 6.0.
Apply 350 W microwave irradiation for 5 minutes.
Rinse slide(s) twice with PBS.
4 x 25 ml 03 315 843 001
DIG Easy Hyb 500 ml 11 603 558 001
NBT/BCIP Stock Solution 8 ml 11 681 451 001
DIG Nucleic Acid Detection Kit 1 kit, Detection of 40 blots of 10 cm x
7. Mizuno T, Zhong X, Rothstein TL. Fas-induced apoptosis in B cells. Apoptosis. 2003
Oct;8(5):451-60.
8. 10: De Fanis U, Romano C, Dalla Mora L, Sellitto A, Guastafierro S, Tirelli A, Bresciano
1. Orphanides G, Reinberg D (2002)
A unified theory of gene
expression. Cell 108: 439-451.
2. Maniatis T, Reed R (2002)
An extensive network of coupling
among
than 4 weeks as progressive precipitation
occurs. We recommend to dissolve aliquots of 5 mg
Fast Red TR in 200 �l redist. H2O. This is sufficient for
20 HNPP/Fast Red TR mix preparations (see below)
HCN pacemaker channels by cyclic AMP: signaling
through dynamic allosteric coupling. Neuron 36(3), 451-461.
Yu, H. G., Lu, Z., Pan, Z., and Cohen, I. S. (2004) Tyrosine kinase inhibition
differentially
discriminate the cat I, II, III, and IV genes by the different sizes of the products:
349, 567, 275, 451 bp, respectively (Fig. 2). It also was powerful enough to detect more
than two types of cat gene
discriminate the cat I, II, III, and IV genes by the different sizes of the products:
349, 567, 275, 451 bp, respectively (Fig. 2). It also was powerful enough to detect more
than two types of cat gene
and TLR4 in recognition of Gram-negative and Gram-positive cell wall components. Immunity 11,
443–451 (1999).
10. Schwadner, R. et al. Peptidoglycan- and lipoteichoic acid-induced cell activation is
ROCK-II R WB IP Pur Rb IgG 200 µg 07-443
Description Clone Species Applications Format Host Quantity Cat. No.
37
Protein
ROKa/ROCK-II, active KA 10 µg 14-451
ROKb/ROCK-I, active
and TLR4 in recognition of Gram-negative and Gram-positive cell wall components. Immunity 11,
443–451 (1999).
10. Schwadner, R. et al. Peptidoglycan- and lipoteichoic acid-induced cell activation is
siRNA plasmid, pKD-ROKa/ROCK-II-v4 H M R RNAi 5 µg 62-075
siRNA plasmid, pKD-ROKa/ROCK-II-v6 H M R RNAi 5 µg 62-076
ROKa/ROCK-II, active KA 10 µg 14-451
ROKb/ROCK-I, active KA 10 µg 14-601
SHC
100 µg/ml 200 ng/ml 10 min
5. Add 2X induction medium (or mock induction medium) directly to inhibitor-treated cells. Immediately return cells to
incubator.
6. Incubate at 37°C and 5% CO2 for
and TLR4 in recognition of
Gram-negative and Gram-positive cell wall components. Immunity 11,
443–451 (1999).
12. Schwadner, R. et al. Peptidoglycan- and lipoteichoic acid-induced cell
activation is