Anti-Phospho-STAT6 (pTyr
641
)
(Item G) or 1x Anti-STAT6 (Item H) to
corresponding well and incubate for 2 hours at
room temperature with shaking.
11. Wash 4 times with 1x Wash Buffer B (200 L each),
then
fluorescent analytical test
instrumentation marketed under the name of Luminex100, 200, HTS.
Page 2 of 7 Millipore 46-641 Rev. 14-SEP-2010
MATERIALS REQUIRED BUT NOT PROVIDED
Reagents
deoxynucleotidyltransferase
Cat. No. 11 732 641 001 100 U
1 x 100 U
40 reactions in a final volume of 50 μl
Cat. No. 11 732 650 001 500 U
2 x 250 U
200 reactions in a
STORAGE/HANDLING: Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
REFERENCE: Nicoletti, A., et al., Hum. Mol. Genet. (1995) 4:641-649.
Important Note:
cells via
CD24, a new member of phosphoinositol-anchored membrane molecules. J. Immunol.
144:638-641.
Shields, J. et al. (1987). In Leukocyte Typing III. A. J. McMichael et al., eds. Oxford
University
) is developed in rabbit
using as immunogen a synthetic peptide corresponding
to amino acids 626-641 of human MTA2/MTA1L1,
conjugated to KLH via an N-terminal added cysteine
residue. The immunizing
for
20 minutes at room temperature.
8. Wash the wells no more than 3 times with
200 l/well of PBS-T. Do not incubate with PBS-T,
just wash.
9. Add 200 l/well of 3,3,5,5-Tetramethylbenzidine
involves the charged residues on
alphaE and alphaI helices in the kinase domain. J.
Cell Biol., 165(5), 641-52 (2004).
2. Baran, C.P. et al., The inositol 5'-phosphatase
SHIP-1 and the Src kinase Lyn negatively
al., J. Food Eng., 263, 366-379
(2019).
10. Werkman, I., et al., J. Neurochem., 156(5),
624-641 (2021).
11. Messner, Holt Nickolas, "An in vivo, ex vivo, and
in vitro exploration of the use
human is effective on mouse and human cells.
References
1 March, C. J. et al. (1985) Nature 315, 641-647.
2 Dinarello, C.A. (1985) J. Clin. Immunol. 5, 287-297.
3 Oppenheim, J.J. et al. (1986) Immunol
Y receptor ligands and
calcium channel antagonists in hypothalamic slices.
J. Neurochem., 73(2), 641–646 (1999).
4. Hanson, E.S., and Dallman, M.F., Neuropeptide Y
(Neuropeptide Y) may integrate
ml of 250 mM MgCl2
Adjust to pH 7.4
Store up to 4 weeks at 2–8 C
• Test Compound Stock Solution: Dissolve
compound at 200 concentration in DMSO and
vortex to mix.
at 2–8 °C
• Test Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
at 2–8 °C
• Test Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.