analysis states the water
content measured by coulometric Karl Fischer Titration
according to ISO 760 specifications.
This oil standard is used for ensuring the accuracy
of Karl Fischer equipment
of Lot Number SLBB7940V:
760 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5 mM
glycerol 2-phosphate, 25 mM MgCl2, 5 mM EGTA, and
2 mM EDTA. Just
bp @ 760 mm:
1. Mark the observed boiling point on chart A.
2. Mark the pressure on chart C.
3. The line drawn from point A to C intersects chart
B to give
bp @ 760 mm:
1. Mark the observed boiling point on chart A.
2. Mark the pressure on chart C.
3. The line drawn from point A to C intersects chart
B to give
bp @ 760 mm:
1. Mark the observed boiling point on chart A.
2. Mark the pressure on chart C.
3. The line drawn from point A to C intersects chart
B to give
bp @ 760 mm:
1. Mark the observed boiling point on chart A.
2. Mark the pressure on chart C.
3. The line drawn from point A to C intersects chart
B to give
activity of 2 × 109
dpm/μg, with different substrate DNAs in 10 min.
Label Content
High Prime • 200 μl
• 5× conc. random primer mix: 1 U/μl Klenow
polymerase, labeling grade, 0.125 mM dATP,
0.125 mM