located in the small cell lung cancer tumor
suppressor gene region. Molec. Cell. Biol., 16, 868-
876 (1996).
2. Ludwig, S. et al., 3pK, a novel mitogen-activated
protein (MAP) kinase-activated protein
located in the small cell lung cancer tumor
suppressor gene region. Molec. Cell. Biol., 16, 868-
876 (1996).
2. Ludwig, S. et al., 3pK, a novel mitogen-activated
protein (MAP) kinase-activated protein
Wild-type C.
perfringens
Mutant plc
targetron
plc-F
plc-R
200 bp 1.1 kb
plc-F
Intron
Specific
No product 876 bp
plc-50/51a target sequence
TAGACTTTAGTTGATGCCCCAGCCCATAGG -
intron
Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
• Test Compound Working
Compound Stock Solution: Dissolve
compound at 200 concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 C.
•
Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
• Test Compound Working
Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
• Test Compound Working
Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
• Test Compound Working
Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
• Test Compound Working
Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
• Test Compound Working
Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
• Test Compound Working
Quantification of
Differential Protein Complex Composition. Rapid
Commun. Mass Spectrom., 18, 869-876 (2004).
8. Stewart, I.I. et al., 18O Labeling: A Tool for
Proteomics., Rapid Commun. Mass Spectrom
Inhibitor 2 solution to
each of tubes 4-6. Mix well by gentle agitation,
avoid bubbles. Incubate all 9 tubes at 37 °C for
5 minutes.
5. Following incubation, add 200 µl of MDR
Fluorescent Cytoplasmic
µL Inhibitor 2 into tubes # 4-6.
Mix thoroughly with gentle pipetting. Avoid bubbling. Incubate all nine
tubes for 5 min at 37 °C.
20. To begin the reaction, add 200 µL of calcein AM solution prepared
≥97% by HPLC.
Cat. No. 217697 1 mg
5 mg
Ref.: Brachwitz, K., et al. 2003. J. Med. Chem. 46, 876.
Cdk Inhibitor, p35
An analog of Olomoucine
(Cat. No. 495620) that acts as a
potent inhibitor of