Mixer
• Deionized water
• Microtiter Plate Reader capable of reading absorbency at 450 nm
and 590 nm
• Orbital Microtiter Plate Shaker
• Absorbent Paper or Cloth
• Optional: DPP IV Inhibitor and
. The enzyme activity is measured spectrophotometrically
by the increased absorbance at 450 nm – 590 nm after acidification of formed products.
Since the increase in absorbance is directly proportional
microtiter plate to remove any residue prior to reading on plate reader.
Read absorbance at 450 nm and 590 nm in a plate reader within 5 minutes and
ensure that there is no air bubbles in any well.
P5704 5579 PRKCB M NP_002729.2 y y y
589 Protein Kinase Cη P8090 5583 PRKCH P NP_006246.2 y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 P NP_002733.2 y y n/d
420 MAP Kinase, Activated/Monophosphorylated
P5704 5579 PRKCB M NP_002729.2 y y y
589 Protein Kinase Cη P8090 5583 PRKCH P NP_006246.2 y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 P NP_002733.2 y y n/d
420 MAP Kinase, Activated/Monophosphorylated
P5704 5579 PRKCB M NP_002729.2 y y y
589 Protein Kinase Cη P8090 5583 PRKCH P NP_006246.2 y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 P NP_002733.2 y y n/d
420 MAP Kinase, Activated/Monophosphorylated
Cystatin A C3095 M y n/d n/d
202 Cytohesin-1 C8979 M y n/d n/d
203 Cytokeratin peptide 4 C5176 M y n/d n/d
204 Cytokeratin CK5 C7785 M y n/d n/d
205 Cytokeratin peptide 7 C6417 M y n/d
=SIGMA�
http://www.sigmaaldrich.com/ProductLookup.html?ProdNo=P8090&Brand=SIGMA�
1
590 Protein Kinase D P3987 5587 PRKD1 NP_002733.2 P y y n/d
591 PKR P0244 5610 EIF2AK2 NP_002750.1 P y
Prkcz NP_071952.1,NP_032886.2 P n/d y y
589 Protein Kinase Cη P8090 5583 PRKCH NP_006246.2 P y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 NP_002733.2 P y y n/d
591 PKR P0244 5610 EIF2AK2 NP_002750.1 P y
healthy cells, the dye
accumulates and aggregates in the mitochondria and
fluoresces red (l em = 585-590 nm). During apoptosis,
the electrochemical gradient across the mitochondrial
membrane breaks down (
0,01/0,02 g Ø 120 mm 200 g, F1 80103901 730
AV412 410 g 0,01 g 149 x 162 mm 200 g, F1 80103530 590
AV412C 410 g 0,01 g 168 x 180 mm 200 g, F1 80103952 790