control SmartFlare (Figure 1). For additional accession
numbers predicted to react with this SmartFlare Probe,
please visit http://www.millipore.com/catalogue/item/SF-
2049
enquiries@oxfordbiomedica.co.uk or BioMedica Inc., 11622 El
Camino Real #100, San Diego CA 92130-2049 USA. LentiVector is
a registered US and European Community trademark of Oxford
BioMedica plc.
enquiries@oxfordbiomedica.co.uk or BioMedica Inc., 11622 El
Camino Real #100, San Diego CA 92130-2049 USA. LentiVector is
a registered US and European Community trademark of Oxford
BioMedica plc.
enquiries@oxfordbiomedica.co.uk or BioMedica Inc., 11622 El
Camino Real #100, San Diego CA 92130-2049 USA. LentiVector is
a registered US and European Community trademark of Oxford
BioMedica plc.
enquiries@oxfordbiomedica.co.uk or BioMedica Inc., 11622 El
Camino Real #100, San Diego CA 92130-2049 USA. LentiVector is
a registered US and European Community trademark of Oxford
BioMedica plc.
enquiries@oxfordbiomedica.co.uk or
BioMedica Inc., 11622 El Camino Real #100, San Diego CA 92130-
2049 USA. LentiVector is a registered US and European Community
trademark of Oxford BioMedica plc.
enquiries@oxfordbiomedica.co.uk or BioMedica Inc., 11622 El
Camino Real #100, San Diego CA 92130-2049 USA. LentiVector is
a registered US and European Community trademark of Oxford
BioMedica plc.
enquiries@oxfordbiomedica.co.uk or BioMedica Inc., 11622 El
Camino Real #100, San Diego CA 92130-2049 USA. LentiVector is
a registered US and European Community trademark of Oxford
BioMedica plc.
enquiries@oxfordbiomedica.co.uk or BioMedica Inc., 11622 El
Camino Real #100, San Diego CA 92130-2049 USA. LentiVector is
a registered US and European Community trademark of Oxford
BioMedica plc.
enquiries@oxfordbiomedica.co.uk or BioMedica Inc., 11622 El
Camino Real #100, San Diego CA 92130-2049 USA. LentiVector is
a registered US and European Community trademark of Oxford
BioMedica plc.
enquiries@oxfordbiomedica.co.uk or BioMedica Inc., 11622 El
Camino Real #100, San Diego CA 92130-2049 USA. LentiVector is
a registered US and European Community trademark of Oxford
BioMedica plc.
enquiries@oxfordbiomedica.co.uk or BioMedica Inc., 11622 El
Camino Real #100, San Diego CA 92130-2049 USA. LentiVector is
a registered US and European Community trademark of Oxford
BioMedica plc.
format.
Day 1
Plate HEK293T cells (13.5 x 106 cells/flask) in
complete DME media 24 hours prior to transfection.
Cells should reach 70%-80% confluency the day of
transfection.
Day 2
1. Thaw the vial
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description
the human target, typically 80–90% in A549 cells, a human epithelial
lung carcinoma cell line.
9
Figure 1. TRC1 and TRC1.5 Lentiviral Plasmid
Vector pLKO.1-puro Features
Name Description