Eur. J. Cell Biol
2008; 87: 581-590.
9. Artym VV, Yamada KM and Mueller SC. ECM degradation
assays for analyzing local cell invasion. Methods Mol Biol
2009; 522:211-219.
10. Xu X, Johnson P and
. The enzyme activity is measured spectrophotometrically
by the increased absorbance at 450 nm – 590 nm after acidification of formed products.
Since the increase in absorbance is directly proportional
measured
spectrophotometrically by the increased absorbency at 450 nm, corrected from the
absorbency at 590 nm, after acidification of formed products. Since the increase in
absorbency is directly proportional
loads, it has been the preferred
method. Generally, initial cell mass
is in the range of 7–30 OD at
590 nm or can reach 5 g/L dry cell
weight (9). Centrifugation can
concentrate cell mass to about 40%
IF, NOT IP human, mouse PC�81 100 µg €211
Anti-IkBa (Ab-2) Rabbit pAb ELISA, IB, IP human, mouse, rat PC142 100 µl €��9
PhosphoDetect™ Anti-IkBa (pSer�2/�6) Mouse mAb (�9A14�1) IB, IP human OP142
SPU4001/TAD7 80104122 403
SPU6001 6 000 g 0,1 g 165 x 142 mm 5 kg + 1 kg, M1 80104030 397 SPU6001/TAD7 80104123 443
SPU6000 6 000 g 1 g 165 x 142 mm 5 kg + 1 kg, M1 80104028 211
P5704 5579 PRKCB M NP_002729.2 y y y
589 Protein Kinase Cη P8090 5583 PRKCH P NP_006246.2 y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 P NP_002733.2 y y n/d
420 MAP Kinase, Activated/Monophosphorylated
P5704 5579 PRKCB M NP_002729.2 y y y
589 Protein Kinase Cη P8090 5583 PRKCH P NP_006246.2 y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 P NP_002733.2 y y n/d
420 MAP Kinase, Activated/Monophosphorylated
P5704 5579 PRKCB M NP_002729.2 y y y
589 Protein Kinase Cη P8090 5583 PRKCH P NP_006246.2 y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 P NP_002733.2 y y n/d
420 MAP Kinase, Activated/Monophosphorylated
wireless range via power plug, available with plugs
for EU, USA, UK
67 x 78 x 125 230
8 900 590 3 Spare batteries (ATEX approved) -- -- --
8 901 105 EasyTEMP Professional Software -- -- --
P8458 P n/d n/d y
588 Protein Kinase Cζ C P0713 P n/d y y
589 Protein Kinase Cη P8090 P y n/d n/d
590 Protein Kinase D P3987 P y y n/d
591 PKR P0244 P y n/d n/d
592 Plakoglobin (Catenin
Prkcz NP_071952.1,NP_032886.2 P n/d y y
589 Protein Kinase Cη P8090 5583 PRKCH NP_006246.2 P y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 NP_002733.2 P y y n/d
591 PKR P0244 5610 EIF2AK2 NP_002750.1 P y
=SIGMA�
http://www.sigmaaldrich.com/ProductLookup.html?ProdNo=P8090&Brand=SIGMA�
1
590 Protein Kinase D P3987 5587 PRKD1 NP_002733.2 P y y n/d
591 PKR P0244 5610 EIF2AK2 NP_002750.1 P y