-
response CHO wt cells were
seeded at 10,000 cells per
well in a 384-well plate 24 hr
before the assay. Cells were
pre-incubated with 1 mM
IBMX for 5 minutes, then
various concentrations of
forskolin were
Instructions
1. Equilibrate assay reagents to room temperature before use.
2. Remove White 384-Well anti-Rabbit Coated Plate/s from foil pouch.
3. Add 30 µL of cAMP Standards 1 through 8
Instructions
1. Equilibrate assay reagents to room temperature before use.
2. Remove White 384-Well anti-Rabbit Coated Plate/s from foil pouch.
3. Add 30 μL of cAMP Standards 1 through 8
in ovarian stimulation for
assisted reproduction? Women’s Heal. 2, 375–384 (2006).
20. Low Endotoxin Recovery (LER): A Review. 1–8 (2014).
21. Williams (Lonza), K. L. Low Endotoxin Recovery (LER)
Wilms tumor 1 7490
ZNF198 zinc fi nger protein 198 7750
ZNF278 zinc fi nger protein 278 23598
ZNF384 zinc fi nger protein 384 171017
ZNFN1A1 zinc fi nger protein subfamily 1A 1 (Ikaros) 10320
Cathepsin D C0715 1509 CTSD M NP_001900.1 y n/d n/d
146 Cathepsin L C2970 1514 CTSL1 M NP_001903.1 y y y
213 DAPK D2178 1611 DAP M NP_004385.1 y n/d n/d
214
Cathepsin D C0715 1509 CTSD M NP_001900.1 y n/d n/d
146 Cathepsin L C2970 1514 CTSL1 M NP_001903.1 y y y
213 DAPK D2178 1611 DAP M NP_004385.1 y n/d n/d
214
Cathepsin D C0715 1509 CTSD M NP_001900.1 y n/d n/d
146 Cathepsin L C2970 1514 CTSL1 M NP_001903.1 y y y
213 DAPK D2178 1611 DAP M NP_004385.1 y n/d n/d
214
18 C1399 M y n/d n/d
211 Cytokeratin peptide 19 C6930 M y n/d n/d
212 Pan Cytokeratin P2871 M y y y
213 DAPK D2178 M y n/d n/d
214 phospho-DAPK (pSer308) D4941
.
REFERENCES
1. Mills, I. G., Praefcke, G. J., Vallis, Y.,
Peter, B. J., Olesen, L. E., Gallop, J.
L., Butler, P. J., Evans, P. R., and
McMahon, H. T. (2003) J. Cell Biol.
160, 213–222.
2. Dong
Cytokeratin peptide 19 C6930 3880 KRT19 NP_002267.2 M y n/d n/d
212 Pan Cytokeratin P2871 M y y y
213 DAPK D2178 1611 DAP NP_004385.1 M y n/d n/d
214 phospho-DAPK (pSer308) D4941
Cytokeratin peptide 19 C6930 3880 KRT19 NP_002267.2 M y n/d n/d
212 Pan Cytokeratin P2871 M y y y
213 DAPK D2178 1611 DAP NP_004385.1 M y n/d n/d
214 phospho-DAPK (pSer308) D4941
.a. 1223-1290 H R IP WB Pur Rb 100 µg AB3882
Anti-phospho-mTOR (Ser2448) M WB APur Rb 100 µg 09-213
siRNA
siRNA plasmid, pKD-mTOR-v4, H RNAi 5 mg 62-302
see also GβL, Raptor, Rheb, Sin1 and