Restriction Endonuclease Bst XI
From Bacillus stearothermophilus XI
Cat. No. 11 117 777 001 250 units (10 U/�l)
Cat. No. 11 117 785 001 1250 units (10 U/�l)
y Version 18
Content version: May 2011
Store
according to international standards like
ISO 8216/IDF 122 (dried milk products) or ISO 7889/IDF 117 (yogurt
products).
• Each 10 g sample is suspended in 90 ml diluent (1/4-strength
Ringer’s solution
ice during use. Use within
2 months.
OXDC Probe – Reconstitute with 220 µL of water. Mix
well by pipetting. Aliquot and store at –20 °C. Use
within 2 months.
Developer – Add 220 L of Methanol Assay
Buffer to the Developer. Mix well. Store at 4 C.
Use within one month once reconstituted. Do not
freeze!
Enzyme Mix and Methanol Probe
diagnostic procedures.
Restriction Endonuclease Ksp I (Sac II)
From Kluyvera species
Cat. No. 11 117 807 001 1000 U (10 U/�l)
y Version 19
Content version: July 2017
Store at �15 to �25°C
G G
and
ligation of sticky- or
blunt-ended DNA frag-
ments.
40 reactions
160 reactions
04 898 117 001
04 898 125 001
rAPid Alkaline
Phosphatase
Dephosphorylation of
5´-phosphate residues
from
and
ligation of sticky- or
blunt-ended DNA frag-
ments.
40 reactions
160 reactions
04 898 117 001
04 898 125 001
rAPid Alkaline
Phosphatase
Dephosphorylation of
5´-phosphate residues
from
sites on different DNAs (2):
Ligation and
recutting assay
Nsi I fragments obtained by complete digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10