analysis of the pBR328 sequence. The 298, 234, and 154 base pair
fragments occur twice.
bp
2,176 1,766 1,230 1,033 653 517 453 394 298 234 220 154
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following base pair (bp) lengths:
The 298, 234, and 154 base pair fragments occur twice.
bp
2,176 1,766 1,230 1,033 653 517 453 394 220
298 234 154
Optimization
The following table provides
Neurobiology M y n/d n/d
152 KIF3A K3513 Neurobiology P y y y
153 Nicastrin N1660 Neurobiology P y n/d n/d
154 SNAP-25 S9684 Neurobiology P n/d y y
155 S-100 beta S2532 Neurobiology M y n/d y
156 S-100
subunit & proform H M R WB Rb 50 µg AB1899 $220
Anti-Caspase 3, active (cleaved) form H M R WB IH IH(P) Web* Rb 50 µg AB3623 $240
Anti-Caspase 3, clone 4-1-18 H WB M
Substrate VII 220 nm by reverse phase HPLC using a C18 column. (Ac-Ile-Glu-Pro-Asp-Trp-Asn-Ala-NH2)
Granzyme B 368067 A colorimetric substrate for granzyme B (kcat/Km = 6.6 x 10
4 M-1s-1). 5 mg
Rapley, R. 1995. The biotechnology and applications of antibody
engineering. Mol. Biotech. 3: 139-154.
Harlow, E. and Lane, D. 1988. Antibodies: A Laboratory Manual.
Cold Spring Harbor Laboratory Press
W5 solution: 154 mM NaCl, 125 mM Ca2Cl, 5 mM KCl, 5 mM glucose, and 2 mM MES,
pH 5.7.
Chill the solution on ice before use.
(4) Modified MMG solution: 0.4 M mannitol, 15 mM MgCl2, and 4 mM MES.