ligated with 1 unit T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithioerythritol, 1 mM
fragments in just 5 min
at +15 to +25 °C.
Kit (40 DNA
ligations)
11 635 379 001
T4 DNA Ligase Ligation of sticky- and
blunt- ended DNA
fragments.
100 U
500 units (1 U/�l)
10 481
fragments in just 5 min
at 15 - 25 °C.
Kit (40 DNA
ligations)
11 635 379 001
T4 DNA Ligase Ligation of sticky- and
blunt- ended DNA
fragments.
100 U
500 units (1 U/�l)
10 481
fragments in just 5 min
at +15 to +25 °C.
Kit (40 DNA
ligations)
11 635 379 001
T4 DNA Ligase Ligation of sticky- and
blunt- ended DNA
fragments.
100 U
500 units (1 U/�l)
10 481
fragments in just 5 min
at 15 - 25 °C.
Kit (40 DNA
ligations)
11 635 379 001
T4 DNA Ligase Ligation of sticky- and
blunt- ended DNA
fragments.
100 U
500 units (1 U/�l)
10 481
T. R. et al (1979) Nucleic Acids Res. 5, 4105.
3 Kessler, C. & Manta, V. (1990) Gene 92, 1-248
4 Rebase The Restriction Enzyme Database:
http://rebase.neb.com
5 Benchmate: http://www.roche-applied-science.com
contact
John Wynne, Director of Business Development,
john_wynne@millipore.com, +44 (791) 248-7375.
coNcLUSIoNS
Addition of a UCOE element into a vector results in
increased frequency of
fragments in just 5 min
at +15 to +25 °C.
Kit (40 DNA
ligations)
11 635 379 001
T4 DNA Ligase Ligation of sticky- and
blunt- ended DNA
fragments.
100 U
500 units (1 U/�l)
10 481
may occur within tissues,
perhaps accounting for its slow onset of action in some
instances. ONO-AE-248, the 11,15-bis-methyl ether of
PGE2, is also a selective EP3 agonist, but appears to be less
potent
Morin, et al., J. Biochem. Pharm., 1983, 32, 553.
31 W. McDowell, et al., Biochem. J., 1987, 248, 523.
32 V.N. Shibaev, Pure Appl. Chem., 1978, 50, 1421.
33 H.H. Higa, J. C. Paulson
IH M IgG1κ 50 µg 16-248
Conjugate
Anti-phospho-Src (Tyr416), Alexa Fluor 555 9A6 H M R IF FC IH M IgG1κ 50 µg 16-249
Conjugate
cDNA
Src cDNA (activated) in pUSEamp TFX 5 µg 21-115
Density at Harvest1 Pure Protein Yield2
Medium Protein OE3 IPTG4 OE IPTG
TB Annexin I 6.8 5.8 240 248
TB Annexin II 5.4 4.9 323 165
TB Tubulin alpha-4 10 8.6 45 15
1 OD600
2 Target proteins were extracted
245 E6AP E8655 M y y y
246 EGF receptor E3138 M y n/d n/d
247 ERK5 (Big MAPK-BMK1) E1523 P y y n/d
248 Elastin E4013 M y n/d n/d
249 ELKS E4531 M y y y
250 Endothelial Cell Protein C Receptor