DMP, 15 cm x 4.6 mm I.D., 5 μm particles
(51098AST)
mobile phase: 10% ethanol in CO2
flow rate: 3 mL/min.
temp.: 35 °C
pressure: 100 bar
det.: UV, 220 nm
inj.: 5
fragments in just 5 min
at 15 - 25°C.
Kit (40 DNA
ligations)
11 635 379 001
T4 DNA Ligase Ligation of sticky- and
blunt- ended DNA
fragments.
100 U
500 units (1 U/�l)
10 481 220
fragments in just 5 min
at 15 - 25°C.
Kit (40 DNA
ligations)
11 635 379 001
T4 DNA Ligase Ligation of sticky- and
blunt- ended DNA
fragments.
100 U
500 units (1 U/�l)
10 481 220
fragments in just 5 min
at 15 - 25°C.
Kit (40 DNA
ligations)
11 635 379 001
T4 DNA Ligase Ligation of sticky- and
blunt- ended DNA
fragments.
100 U
500 units (1 U/�l)
10 481 220
phospho-Tyrosine – 05-777 M
phospho-Tyrosine – 06-427 Rb
PLC-γ-1 135 05-163 M
PP2A 36 05-421 M
M-mouse, Rb-rabbit
** tested using investigator-supplied antibody
Proteins have also been
Chromaster-5430 Diode Array Detector, UV=220 nm
Cell: 13 μL
Flow Rate: 1.5 mL/min
Mobile Phase: Buffer: Dissolve 0.385 g of ammonium acetate in 1000 ml water (5 mM).
Mix Acetonitrile and Buffer 85:15
Detection: 220 nm
Peak identification: 1. Gly-Tyr
2. Val-Tyr-Val
3. Met enkephalin
4. Leu enkephalin
5. Angiotensin II
Gradient: 1 min (0.5/0.25 min) 10% B
10 min (5/2.5 min) 10 – 20% B
5 min (2.5/