adding 2 mL of 20% Tween® 20 (v/v)
(Catalog # 20-246) and 40 mL of 20X TBS (Catalog # 20-190) to 758 mL of Milli-Q or distilled
water. Store at room temperature.
3. Wortmannin (Catalog #CS203298
Distilled Water.
2. 800 mL of 1X Wash Buffer: Combine 2 mL 20% Tween® 20, 40 mL 20X TBS, and 758 mL
Distilled Water.
3. 30 mL Assay Blocking Buffer: Combine 1 mL of