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23184
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Y Zhang et al.
IET nanobiotechnology, 6(2), 76-80 (2012-05-09)
With the wide application of nanomaterials, the quantification of functional groups on nanomaterial surface becomes more and more necessary. A heterogeneous 9-fluorenylmethoxy carbonyl chloride (Fmoc-Cl) fluorescent method using an aqueous solution was established to determinate amino groups on nanomaterial surface.
Carlo Siciliano et al.
The Journal of organic chemistry, 77(23), 10575-10582 (2012-11-14)
The study describes a new "one-pot" route to the synthesis of N-9-fluorenylmethyloxycarbonyl (Fmoc) α-amino diazoketones. The procedure was tested on a series of commercially available free or side-chain protected α-amino acids employed as precursors. The conversion into the title compounds
Xuezheng Song et al.
Analytical biochemistry, 395(2), 151-160 (2009-08-25)
Glycan microarray technology has become a successful tool for studying protein-carbohydrate interactions, but a limitation has been the laborious synthesis of glycan structures by enzymatic and chemical methods. Here we describe a new method to generate quantifiable glycan libraries from
Yiqiang Chen et al.
Journal of separation science, 32(21), 3620-3626 (2009-10-21)
A sensitive and robust HPLC method was developed for the determination of kanamycin (Kan) in swine tissues. Kan was extracted from tissue with 10% TCA (w/v) solution and cleaned up with MCX SPE column. The purified extract was derivatized with
G Csikó et al.
Journal of veterinary pharmacology and therapeutics, 37(4), 406-412 (2014-03-19)
Butyrate, a commonly applied feed additive in poultry nutrition, can modify the expression of certain genes, including those encoding cytochrome P450 (CYP) enzymes. In comparative in vitro and in vivo experiments, the effect of butyrate on hepatic CYP genes was
H Brückner et al.
Amino acids, 42(2-3), 877-885 (2011-07-30)
The reagent 9-fluorenylmethoxycarbonyl chloride (Fmoc-Cl) was used for the pre-column derivatization of the biogenic amines (BAs) cadaverine (Cad), histamine (Him), octopamine (Ocp), phenylethylamine (Pea), putrescine (Put), spermidine (Spd), spermine (Spm), tyramine (Tym) and the internal standard 1,6-diaminohexane (Dhx). The resulting
Valentin Lozanov et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 860(1), 92-97 (2007-11-07)
A liquid chromatography method for simultaneous analysis of amino acids, polyamines, catecholeamines and metanephrines in human body fluids after derivatization with 9-fluorenylmethyloxycarbonyl chloride was developed. The chromatographic behavior of analytes at different pH of mobile phase was studied. Successful baseline
En-Ping Lin et al.
Talanta, 114, 297-303 (2013-08-21)
This study proposes a sensitive method for the simultaneous separation and concentration of 9 pairs of amino acid enantiomers by combining poly(ethylene oxide) (PEO)-based stacking, β-cyclodextrin (β-CD)-mediated micellar electrokinetic chromatography (MEKC), and 9-fluoroenylmethyl chloroformate (FMOC) derivatization. The 9 pairs of
Sourav Majumdar et al.
Biochimie, 103, 50-60 (2014-04-17)
In this study, biochemical and pharmacological characterization of Brevithrombolase, a fibrinolytic serine protease purified from Brevibacillus brevis strain FF02B has been reported. An assessment of its thrombolytic potency has also been made. The molecular mass of this monomeric protease was
A Korös et al.
Journal of chromatography. A, 1203(2), 146-152 (2008-08-05)
A high-performance liquid chromatographic method (HPLC) with combined diode array and fluorescence detection of amino acids and amines in various cheese samples is described. The proposal is based on acidic deproteinization, derivatization and gradient optimization studies, resulting in the identification
Jiawen Yu et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 25(7), 855-859 (2009-07-18)
A simple and sensitive reversed-phase liquid chromatographic method, based on the precolumn derivatization with 9-fluorenylmethyl chloroformate, was developed for the determination of myriocin. The derivatization reaction was performed in organic solvents of pyridine and tetrahydrofuran at 40 degrees C. Several
Hamed Gilzad Kohan et al.
PloS one, 10(5), e0126786-e0126786 (2015-05-16)
The aim of this study was to synthesize a peptide prodrug of glucosamine (GlcN) with increased gut permeability through the gut peptide transporter 1 (PepT1). Glycine-Valine ester derivative of GlcN (GVG) was synthesised using solid phase synthesis followed by characterization
Yang Li et al.
Macromolecular bioscience, 15(1), 52-62 (2014-12-06)
To address the downside of conventional photo-patterning which can alter the chemical composition of protein scaffolds, we developed a non-covalent photo-patterning strategy for gelatin (denatured collagen) hydrogels that utilizes UV activated triple helical hybridization of caged collagen mimetic peptide (caged
Ibolya Molnár-Perl
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(17-18), 1241-1269 (2011-03-05)
An overview is presented on the advancement of the two most frequently used derivatization protocols applying the o-phthalaldehyde (OPA)-thiol and the fluorenylmethyloxycarbonyl (FMOC) chloride reagents, prior to the high performance liquid chromatographic analysis of amino acids. This review pays special
Kun Qian et al.
Analytica chimica acta, 635(2), 222-226 (2009-02-17)
A pre-column derivatization high-performance liquid chromatographic method for glyphosate analysis has been developed. Derivatization of glyphosate was performed with 4-chloro-3,5-dinitrobenzotrifluoride (CNBF). In pH 9.5 H(3)BO(3)-Na(2)B(4)O(7) media, the reaction of glyphosate with CNBF completed at 60 degrees C for 30min. The
Bart Hens et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 63, 233-242 (2014-07-30)
The purpose of this study was to explore the transfer of drug solutions from stomach to small intestine and its impact on intraluminal drug concentrations in humans. The collected intraluminal data were used as reference to evaluate simulations of gastrointestinal
Mei-Fen Xie et al.
Journal of separation science, 34(3), 241-246 (2011-01-27)
In this study, we investigated a simple, sensitive and reliable liquid chromatography-fluorescence detection method for the determination of memantine hydrochloride in rat plasma which was based on derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl). For the first time, FMOC-Cl was introduced into
Ying Liu et al.
Journal of chromatographic science, 47(10), 925-930 (2009-11-26)
A sensitive and reliable method has been developed to determine synephrine in a famous purgative Chinese medicinal prescription, da-cheng-qi decoction. After purified by solid-phase extraction (SPE), the decoction was derivatized with 9-fluorenylmethyl chloroformate in borate buffer and analyzed by reversed-phase
Shengyuan Xiao et al.
Analytical biochemistry, 384(1), 20-26 (2008-10-11)
Methylamine and aminoacetone are endogenous aliphatic amines found in human blood and urine. They can be oxidized by semicarbazide-sensitive amine oxidase (SSAO), leading to the production of toxic aldehydes such as formaldehyde and methylglyoxal as well as hydrogen peroxide and
E J Miller et al.
Analytical biochemistry, 190(1), 92-97 (1990-10-01)
A recently described procedure for amino acid analyses has been modified and adapted for use in quantitating the unique mixture of products commonly found in hydrolysates of the collagens. The method involves precolumn derivatization of hydrolysates with 9-fluorenylmethyl chloroformate (FMOC-CL)
Guoying Cao et al.
The Journal of antimicrobial chemotherapy, 62(5), 1009-1014 (2008-09-04)
The purpose of this study was to develop a specific, sensitive, accurate and reproducible high-performance liquid chromatographic (HPLC) method to measure polymyxin B in human plasma. Derivatization of polymyxin B with fluorescent 9-fluorenylmethyl chloroformate (FMOC-Cl) was performed in the same
K Puzio et al.
Journal of chromatography. A, 1361, 1-8 (2014-08-26)
Three molecularly imprinted polymers (MIPs) have been synthesized in order to bind efficiently glyphosate (GLY) in natural waters (mineral and underground). Since the target analyte is polar and hydrophilic, electrostatic interactions and hydrogen bonds have been favored with two templates
William L Scott et al.
Journal of combinatorial chemistry, 11(1), 34-43 (2008-12-25)
For the successful implementation of Distributed Drug Discovery (D(3)) (outlined in the accompanying Perspective), students, in the course of their educational laboratories, must be able to reproducibly make new, high quality, molecules with potential for biological activity. This article reports
J Bernal et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878(31), 3290-3296 (2010-11-26)
A simple and fast method has been developed and validated to measure glyphosate (GLYP) and aminomethylphosphonic acid (AMPA) in rat plasma based on reversed-phase high performance liquid chromatography (RP-HPLC) coupled to fluorescence (FLD) and electrospray ionization mass spectrometry (ESI-MS) detection.
P Fürst et al.
Journal of chromatography, 499, 557-569 (1990-01-19)
Reversed-phase high-performance liquid chromatography (RP-HPLC) is a powerful method for assaying physiological amino acid concentrations in biological fluids. Four pre-column derivatization methods, with o-phthaldialdehyde (OPA), 9-fluorenylmethyl chloroformate (FMOC-Cl), phenyl isothiocyanate (PITC) and 1-dimethylaminonaphthalene-5-sulphonyl chloride (dansyl-Cl), were assessed with respect to
Jing Zhu et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 31(22), 1865-1868 (2007-02-09)
To establish a reversed-phase high performance liquid chromatorgraphy (RP-HPLC) method for detecting the dencichine in Panax notoginseng extracts and drug preparations. Dencichine was extracted with the borate buffer (pH 9. 18) and the clear supernatant was used for the derivatization.
Z X Xie et al.
Journal of animal physiology and animal nutrition, 99(3), 492-500 (2014-10-01)
Increased levels of plasma free amino acids (pFAAs) can disturb the blood glucose levels in patients with obesity, diabetes mellitus and metabolic syndrome (MS) and are associated with enhanced protein oxidation. Oxidation of proteins, especially in the muscles, can promote
Sri Puvanesvari Gannasin et al.
Food chemistry, 182, 292-301 (2015-04-07)
Tamarillo (Solanum betaceum Cav.) is an underutilised fruit in Malaysia. The fruit, however, contains good proportions of soluble fibre, protein, starch, anthocyanins and carotenoids. Amongst the fruits, only tamarillo mesocarp contains both polar (anthocyanins) and non-polar (carotenoids) pigments. The ability
Joonho Bae et al.
International journal of pharmaceutics, 480(1-2), 37-47 (2015-01-18)
We prepared an injectable depot system for the long-term delivery of alendronate using a solid/water/oil/water multiple emulsion technique with poly(lactic-co-glycolic acid) as a carrier. The microparticles were spherical with smooth surfaces, ranging from 20 to 70 μm in size. The
Ingrid Freuze et al.
Journal of chromatography. A, 1175(2), 197-206 (2007-11-21)
Experimental and theoretical influence of multivalent cations on the analysis of glyphosate and aminomethyl phosphonic acid (AMPA) was studied in pure water and in one surface water. The procedure chosen, based on derivatization with FMOC-Cl, HPLC separation, and fluorescence detection
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