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Hyun-Dong Shin et al.
Bioresource technology, 118, 367-373 (2012-06-19)
Escherichia coli has been engineered to produce a variety of biofuel and biorefinery products. However, it can only produce these products from simple sugars, requiring large amounts of enzymes to depolymerize cellulose into monomer sugars. Engineering E. coli to directly
Journal of the American Chemical Society, 115, 4602-4602 (1993)
Yuansheng Xue et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 27(12), 1742-1748 (2012-04-18)
The production of 2, 3-butanediol and succinic acid by a moderate halophile under anaerobic condition was investigated. This halophile, termed Salinivibrio YS, was isolated from the solid samples collected from Aydingkol Lake. Based on the single factor experiment, the parameters
Mingshou Lu et al.
Journal of microbiology and biotechnology, 22(5), 659-667 (2012-05-09)
2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway
Gen Lin Zhang et al.
Biotechnology letters, 34(8), 1519-1523 (2012-05-02)
The budC gene encoding the meso-2,3-BDH from Klebsiella pneumoniae XJ-Li was expressed in E. coli BL21 (DE3) pLys. Hypothetical amino acid sequence alignments revealed that the enzyme belongs to the short chain dehydrogenase/reductase family. After purification and refolding, the recombinant
Jong Myoung Park et al.
Microbial cell factories, 12, 20-20 (2013-02-26)
Klebsiella oxytoca, a Gram-negative, rod-shaped, and facultative anaerobic bacterium, is one of the most promising 2,3-butanediol (2,3-BD) producers. In order to improve the metabolic performance of K. oxytoca as an efficient biofactory, it is necessary to assess its metabolic characteristics
David P Eisenberg et al.
Cryobiology, 65(2), 117-125 (2012-05-15)
This study explores physical effects associated with the application of cryopreservation via vitrification using a class of compounds which are defined here as synthetic ice modulators (SIMs). The general classification of SIMs includes molecules that modulate ice nucleation and growth
Xiaolin Shen et al.
Journal of industrial microbiology & biotechnology, 39(11), 1725-1729 (2012-07-27)
This work describes the production of (R,R)-2,3-butanediol in Escherichia coli using glycerol by metabolic engineering approaches. The introduction of a synthetic pathway converting pyruvate to (R,R)-2,3-butanediol into wild-type E. coli strain BW25113 led to the production of (R,R)-2,3-butanediol at a
Sung-Geun Jung et al.
Applied microbiology and biotechnology, 97(5), 1997-2007 (2012-07-27)
Klebsiella species are the most extensively studied among a number of 2,3-butanediol (2,3-BDO)-producing microorganisms. The ability to metabolize a wide variety of substrates together with the ease of cultivation made this microorganisms particularly promising for the application in industrial-scale production
Burk, M.J. et al.
Tetrahedron Asymmetry, 2, 569-569 (1991)
Meng Wang et al.
Biotechnology letters, 34(10), 1877-1885 (2012-06-21)
Acetoin is widely used in food and other industries. A bdhA and acoA double-knockout strain of Bacillus subtilis produced acetoin at 0.72 mol/mol, a 16.4 % increased compared to the wild type. Subsequent overexpression of the alsSD operon enhanced the
Predominant Conformations of (2 R, 3 R)-(-)-2, 3-Butanediol.
Wang F and Polavarapu PL.
The Journal of Physical Chemistry A, 105(29), 6991-6997 (2001)
Yuanzhi He et al.
Molecules (Basel, Switzerland), 23(3) (2018-03-23)
(3S)-Acetoin and (2S,3S)-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. However, their production by fermentative methods is difficult to perform. This study aimed to develop a whole-cell biocatalysis strategy for the production of
John W K Oliver et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(4), 1249-1254 (2013-01-09)
Conversion of CO(2) for the synthesis of chemicals by photosynthetic organisms is an attractive target for establishing independence from fossil reserves. However, synthetic pathway construction in cyanobacteria is still in its infancy compared with model fermentative organisms. Here we systematically
Jinshan Li et al.
Applied microbiology and biotechnology, 97(2), 585-597 (2012-08-07)
Paenibacillus polymyxa can produce the (R,R)-stereoisomer of 2,3-butanediol (2,3-BDL) which is industrially very useful. Two important factors affecting (R,R)-BDL production by P. polymyxa ATCC 12321, medium composition, and addition of acetic acid to the culture were investigated in this study
Sangjun Jeon et al.
Journal of bioscience and bioengineering, 117(4), 464-470 (2013-10-23)
This study presents a new and effective downstream process to recover 2,3-butanediol (2,3-BD) from fermentation broth which is produced by a recombinant Klebsiella pneumoniae strain. The ldhA-deficient K. pneumoniae strain yielded about 90 g/L of 2,3-BD, along with a number
Eduard Calam et al.
Chemico-biological interactions, 202(1-3), 195-203 (2013-01-09)
The α-hydroxy ketones are used as building blocks for compounds of pharmaceutical interest (such as antidepressants, HIV-protease inhibitors and antitumorals). They can be obtained by the action of enzymes or whole cells on selected substrates, such as diketones. We have
Stolle, A. et al.
Tetrahedron Letters, 35, 3521-3521 (1994)
Hsin-Chih Lai et al.
Journal of leukocyte biology, 92(4), 807-814 (2012-07-18)
The natural compound 2,3-BTD has diverse physiological effects in a range of organisms, including acting as a detoxifying product of liver alcohol metabolism in humans and ameliorating endotoxin-induced acute lung injury in rats. In this study, we reveal that 2,3-BTD
Engineered Serratia marcescens for efficient (3R)-acetoin and (2R, 3R)-2, 3-butanediol production.
Bai F, et al.
Journal of Industrial Microbiology & Biotechnology, 42(5), 779-786 (2015)
Marshall, J.A. Xie, S.
The Journal of Organic Chemistry, 60, 7230-7230 (1995)
Sang Heum Shin et al.
Journal of bacteriology, 194(10), 2736-2737 (2012-04-27)
Here we report the full genome sequence of Klebsiella pneumoniae KCTC 2242,consisting of a 5.26-Mb chromosome (57.6% GC%; 5,035 genes [4,923 encoding known proteins, 112 RNA genes]) and a 202-kb plasmid (50.2% GC%; 229 genes [229 encoding known proteins]).
Burk, M.J. et al.
Journal of the American Chemical Society, 115, 10125-10125 (1993)
Borim Kim et al.
Journal of microbiology and biotechnology, 22(9), 1258-1263 (2012-07-21)
2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae
Sung-Hyuk Han et al.
New biotechnology, 30(2), 166-172 (2012-09-20)
2,3-Butanediol (2,3-BDO) is a value-added chemical with great potential for the industrial production of synthetic rubber, plastic and solvent. For microbial production of 2,3-BDO, in this study, Klebsiella oxytoca NBRF4 was constructed by chemical mutation and screening against NaBr, NaBrO(3)
Tetrahedron Asymmetry, 17, 993-993 (2006)
Yuzhen Zhang et al.
Bioresource technology, 121, 100-104 (2012-08-04)
Pretreatment is necessary for lignocellulose to achieve a highly efficient enzymatic hydrolysis and fermentation. However, coincident with pretreatment, compounds inhibiting microorganism growth are formed. Some tissues or cells, such as thin-walled cells that easily hydrolyze, will be excessively degraded because
Bram Vivijs et al.
International journal of food microbiology, 175, 36-44 (2014-02-18)
The mechanisms by which Enterobacteriaceae can survive or grow at low pH are of interest because members of this family are increasingly linked to problems of spoilage and foodborne infection related to mildly acidic foods. In this work, we investigated
Tao-Wei Yang et al.
Applied microbiology and biotechnology, 97(17), 7651-7658 (2013-06-26)
Cultivation in glycerol instead of sugars inhibits 2,3-butanediol (2,3-BD) production by Bacillus amyloliquefaciens. In this study, we report that B. amyloliquefaciens readily produces 2,3-BD from biodiesel-derived glycerol in the presence of beet molasses as a co-substrate. Unexpectedly, the molasses stimulated
Yanjie Zhang et al.
Bioresource technology, 130, 256-260 (2013-01-12)
A new strain, FMME044, exhibited a remarkable ability to synthesize acetoin and was identified as Bacillus amyloliquefaciens. The following characteristics of enzyme activity were found: 2,3-butanediol was reverse transformed to acetoin upon depletion of glucose; lower agitation speeds favored 2,3-butanediol
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