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EK,CS
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Methodology: Process and Product Optimization
solution. The buffer solution is prepared by dissolving 146 g anhydrous sodium
acetate, or 243 g sodium acetate trihydrate in 400 mL of reagent water (Section 7.4),
followed by addition of 480
common proteins
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sequence of basic chitinase
(RSC-c) from rye (Secale cereale) seeds.
The RSC-c molecule consists of 243
amino acid residues and three disulfide
bonds (C23/C85, C97/C105, and
C223/C236) (1). To understand
n/d
241 E2F1 E8901 1869 E2F1 M NP_005216.1 y y y
242 E2F2 E8776 1870 E2F2 M NP_004082.1 y n/d n/d
243 E2F3 E8651 1871 E2F3 M NP_001940.1 y n/d n/d
244 E2F4 E8526 1874 E2F4 M NP_001941.2 y n/d n/d
245 E6AP
n/d
241 E2F1 E8901 1869 E2F1 M NP_005216.1 y y y
242 E2F2 E8776 1870 E2F2 M NP_004082.1 y n/d n/d
243 E2F3 E8651 1871 E2F3 M NP_001940.1 y n/d n/d
244 E2F4 E8526 1874 E2F4 M NP_001941.2 y n/d n/d
245 E6AP
n/d
241 E2F1 E8901 1869 E2F1 M NP_005216.1 y y y
242 E2F2 E8776 1870 E2F2 M NP_004082.1 y n/d n/d
243 E2F3 E8651 1871 E2F3 M NP_001940.1 y n/d n/d
244 E2F4 E8526 1874 E2F4 M NP_001941.2 y n/d n/d
245 E6AP
sequence.
B. Renilla luciferase assays of enhancer activity in COS-1, 293 cells, and BHK cells. A 243-bp SV40 enhancer was cloned into
the Kpn I and Pml I sites of pMLuc-2 and tested against the empty
E9026 P y n/d n/d
240 Anti Cy3+Cy5 C0992 M NA NA NA
241 E2F1 E8901 M y y y
242 E2F2 E8776 M y n/d n/d
243 E2F3 E8651 M y n/d n/d
244 E2F4 E8526 M y n/d n/d
245 E6AP E8655 M y