mixed with 900 µl of methanol with 1% formic acid. The
sorbent was washed with 1 ml of methanol with 1% ammonium
formate, followed by 1 ml of methanol. The phospholipids were
eluted with two 1 ml aliquots
immediately before setting up
the assay.
1. Dilute the 10X concentrated HRP wash buffer 10 fold by mixing the entire
contents of both buffer bottles with 900 mL de-ionized or glass distilled
water.