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Mark A Hooks et al.
The Biochemical journal, 463(2), 309-317 (2014-07-26)
Arabidopsis thaliana has three genes that encode distinct aconitases (ACO), but little is known about the function of each isoenzyme during plant development. In newly emerged seedlings of Arabidopsis, transcript and protein levels for ACO3 were selectively induced to yield
Nilanjana Chatterjee et al.
PloS one, 7(8), e42063-e42063 (2012-08-23)
Accurate estimation of template--DNA or RNA by real time PCR is dependent on the amplification efficiency (F) of the reaction. The analytical equation describing the kinetics of PCR that is influenced by template re-annealing is formulated. It predicts the gradual
Björn Mückschel et al.
Applied and environmental microbiology, 78(24), 8531-8539 (2012-10-02)
In this study, we investigated the metabolism of ethylene glycol in the Pseudomonas putida strains KT2440 and JM37 by employing growth and bioconversion experiments, directed mutagenesis, and proteome analysis. We found that strain JM37 grew rapidly with ethylene glycol as
Liang Chen et al.
Biomedical materials (Bristol, England), 7(4), 045014-045014 (2012-06-13)
The purpose of this research was to investigate the influence of the glyoxylic acid (GA) modification of hydroxyapatite (HAP) nanofibers on their dispersion in bisphenol A glycidyl methacrylate (BisGMA)/triethylene glycol dimethacrylate (TEGDMA) dental composites and also to investigate the mechanical
Jin Guan et al.
Se pu = Chinese journal of chromatography, 30(1), 107-110 (2012-06-07)
A method for the simultaneous determination of glyoxalate and oxalate by capillary zone electrophoresis (CZE) was developed. The influences of type, concentration and pH of the running buffer, and the applied voltage on separation were investigated. Glyoxalate and oxalate were
Andrei Tintu et al.
Annals of clinical biochemistry, 50(Pt 1), 70-72 (2012-11-07)
Metabolites of ethylene glycol (EG) can cross-react in l-lactate assays, leading to falsely elevated serum lactate levels in case of EG intoxication. In this study, we evaluated the effects of EG and its metabolites on routinely used lactate measuring methods.
A Arvesen et al.
VASA. Zeitschrift fur Gefasskrankheiten, 30(3), 176-183 (2001-10-05)
Functional and pathological improvements following rapid rewarming in 42 degrees C water was compared with alterations following slow thawing at room temperature (22 degrees C) after frostbite (-9 degrees C, 15 minutes) in vivo of the rabbit central ear artery.
Guillermo G Zampar et al.
Molecular systems biology, 9, 651-651 (2013-04-04)
The diauxic shift in Saccharomyces cerevisiae is an ideal model to study how eukaryotic cells readjust their metabolism from glycolytic to gluconeogenic operation. In this work, we generated time-resolved physiological data, quantitative metabolome (69 intracellular metabolites) and proteome (72 enzymes)
Huangdi Feng et al.
Organic letters, 14(7), 1942-1945 (2012-03-30)
An efficient and novel copper-mediated protocol for the synthesis of 3-amino-1,4-enynes from glyoxylic acid, an amine, and an alkyne was developed. This new reaction involving two sequential C-C bond formations is air and moisture tolerant and proceeds via a tandem
Parantap Sarkar et al.
Chemical communications (Cambridge, England), 49(68), 7552-7554 (2013-07-23)
Twofold Perkin condensation of 2,5-dibromophenylene-1,4-diacetic acid with arylglyoxylic acids followed by cyclo-dehydrobromination leads to dipyreno- and diperyleno-anthracene tetraesters and diimides. The imides show surprisingly large absorption shifts versus the esters, illustrating that electron-withdrawing substituents at the anthracene unit efficiently impart
Florian Couvidat et al.
Environmental science & technology, 47(2), 914-922 (2012-11-30)
A molecular surrogate representation of secondary organic aerosol (SOA) formation is used to investigate the effect of aqueous-phase (in clouds and particles) chemical processing and wet deposition on SOA atmospheric concentrations. To that end, the hydrophilic/hydrophobic organic (H(2)O) model was
Songyan Gao et al.
Journal of ethnopharmacology, 166, 323-332 (2015-03-22)
Orthosiphon stamineus (OS), a traditional Chinese herb, is often used for promoting urination and treating nephrolithiasis. Urolithiasis is a major worldwide public health burden due to its high incidence of recurrence and damage to renal function. However, the etiology for
Michelle M Giffin et al.
Journal of bacteriology, 194(5), 1045-1054 (2012-01-03)
The putative glycine dehydrogenase of Mycobacterium tuberculosis catalyzes the reductive amination of glyoxylate to glycine but not the reverse reaction. The enzyme was purified and identified as the previously characterized alanine dehydrogenase. The Ald enzyme was expressed in Escherichia coli
Gordon J Hoover et al.
Biochimica et biophysica acta, 1834(12), 2663-2671 (2013-10-01)
NADPH-dependent glyoxylate reductases from Arabidopsis thaliana (AtGLYR) convert both glyoxylate and succinic semialdehyde into their corresponding hydroxyacid equivalents. The primary sequence of cytosolic AtGLYR1 reveals several sequence elements that are consistent with the β-HAD (β-hydroxyacid dehydrogenase) protein family, whose members
H Tainio et al.
Histochemistry, 85(2), 117-120 (1986-01-01)
The innervation of human axillary sweat glands was studied by using the specific SPG (sucrose-potassium phosphate-glyoxylic acid) catecholamine histofluorescence method and the peroxidase-antiperoxidase (PAP) immunocytochemical method. The present results demonstrated that human sweat glands are surrounded by nerves containing a
En-Zhi Shen et al.
Nature, 508(7494), 128-132 (2014-02-14)
It has been theorized for decades that mitochondria act as the biological clock of ageing, but the evidence is incomplete. Here we show a strong coupling between mitochondrial function and ageing by in vivo visualization of the mitochondrial flash (mitoflash)
Progressive changes in glycine and glycine derivatives in plasma and cerebrospinal fluid after transurethral prostatic resection.
C Périer et al.
Clinical chemistry, 36(12), 2152-2153 (1990-12-01)
Raphael Voges et al.
Journal of proteomics, 75(9), 2660-2669 (2012-04-06)
Selected reaction monitoring allows quantitative measurements of proteins over several orders of magnitude in complex biological samples. Here we present a targeted approach for quantification of 19 enzymes from Corynebacterium glutamicum applying isotope dilution mass spectrometry coupled to high performance
F Naghizadeh et al.
Clinical biochemistry, 9(2), 65-66 (1976-04-01)
The reduction of pyruvate, 2-oxobutyrate, hydroxy-pyruvate and glyoxylate under optimal conditions at 37 degrees by extracts of human heart, kidney, pancreas, skeletal muscle and liver has been examined. The ratios of reduction rates, particularly of glyoxylate and 2-oxobutyrate reveal a
Saurabh Awasthi et al.
Applied biochemistry and biotechnology, 177(5), 1013-1024 (2015-08-16)
Increased burden of advanced glycation end-products (AGEs) in case of hyperglycemic conditions leads to the development of retinopathy, nephropathy, and cardiovascular and neurological disorders such as Alzheimer's disease. AGEs are considered as pro-oxidants, and their accumulation increases the oxidative stress.
W L Davis et al.
The Anatomical record, 234(4), 461-468 (1992-12-01)
The enzymatic activities unique to the glyoxylate cycle of higher plants and certain lower invertebrates, isocitrate lyase and malate synthase, have been demonstrated in homogenates prepared from human liver. Human liver can also carry out cyanide-insensitive fatty acid oxidation from
E Mentasti et al.
Journal of chromatography, 417(2), 253-260 (1987-07-03)
A high-performance liquid chromatographic method for the determination of carbonyl compounds, namely aldehydes, ketones and keto acids in urine, has been developed. Pre-column derivatization with 2,4-dinitrophenylhydrazine offers sufficient sensitivity for the determination of glyoxylic acid in urine with a detection
Venkata S K Indurthi et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 59, 58-68 (2014-04-23)
Glycation alters the drug binding properties of serum proteins and could affect free drug concentrations in diabetic patients with elevated glycation levels. We investigated the effect of bovine serum albumin glycation by eight physiologically relevant glycation reagents (glucose, ribose, carboxymethyllysine
Myung-Il Kim et al.
PloS one, 7(12), e52066-e52066 (2013-01-04)
Nitrogen metabolism is one of essential processes in living organisms. The catabolic pathways of nitrogenous compounds play a pivotal role in the storage and recovery of nitrogen. In Escherichia coli, two different, interconnecting metabolic routes drive nitrogen utilization through purine
Yi Li et al.
Organic letters, 14(8), 2062-2065 (2012-04-07)
An efficient and straightforward method for the preparation of highly enantiomerically enriched β,γ-unsaturated α-amino acid derivatives by a Lewis acid promoted diastereoselective Petasis reaction of vinylboronic acid, N-tert-butanesulfinamide, and glyoxylic acid has been developed. The synthetic utilities of the approach
Antonio Di Matteo et al.
Biochemical genetics, 50(11-12), 908-921 (2012-08-23)
In recent years, interest in tomato breeding for enhanced antioxidant content has increased as medical research has pointed to human health benefits from antioxidant dietary intake. Ascorbate is one of the major antioxidants present in tomato, and little is known
Vijai Bhadauria et al.
PloS one, 7(4), e36266-e36266 (2012-05-05)
The role of β-oxidation and the glyoxylate cycle in fungal pathogenesis is well documented. However, an ambiguity still remains over their interaction in peroxisomes to facilitate fungal pathogenicity and virulence. In this report, we characterize a gene encoding an alanine
Xueyang Feng et al.
PLoS computational biology, 8(2), e1002376-e1002376 (2012-02-10)
Shewanella oneidensis MR-1 sequentially utilizes lactate and its waste products (pyruvate and acetate) during batch culture. To decipher MR-1 metabolism, we integrated genome-scale flux balance analysis (FBA) into a multiple-substrate Monod model to perform the dynamic flux balance analysis (dFBA).
F J Bruzzese et al.
Biomaterials, artificial cells, and artificial organs, 18(2), 143-156 (1990-01-01)
Hemoglobin obtained from out-dated human blood was stripped of 2,3-diphosphoglycerate and modified with the crosslinking agents glyoxalic acid, 1,2-cyclohexadione, or fumarate to stabilize the tetramer. The resulting hemoglobins, which show alterations in their oxygen transport capability, have been studied in
Alessio Aprile et al.
BMC genomics, 14, 821-821 (2013-11-26)
Durum wheat often faces water scarcity and high temperatures, two events that usually occur simultaneously in the fields. Here we report on the stress responsive strategy of two durum wheat cultivars, characterized by different water use efficiency, subjected to drought
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