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Liming Xu et al.
Research in microbiology, 165(2), 91-101 (2014-01-28)
The mycobacterial peptidoglycan has structure and biosynthetic pathways to similar those of other bacteria. UDP-N-acetylglucosamine enolpyruvyle transferase (MurA) catalyzes the first reaction in the biosynthesis of peptidoglycan. The MurA enzyme has been identified from various bacterial species, but the in-depth
R Senna et al.
Plant physiology and biochemistry : PPB, 44(7-9), 467-473 (2006-10-07)
Acid phosphatase activity (orthophosphoric-monoester phosphohydrolase, EC 3.1.3.2) increased during the first 24 h of maize (Zea mays) seed germination. The enzyme displayed a pH optimum of 4.5-5.5. Catalytic activity in vitro displayed a linear time course (60 min) and reached
S Kovács et al.
Journal of animal physiology and animal nutrition, 99 Suppl S1, 1-12 (2015-04-14)
The purpose of this study was to investigate the influence of exposure to ultraviolet radiation (UVB) on vitamin D status, intestinal calcium absorption and bone metabolism in growing sheep and goats. The hypothesis was that growing sheep and goats are
Negative staining
Harris JR et al.
Electron Microscopy in Biology (1991)
Rishil J Kathawala et al.
Chinese journal of cancer, 34(3), 115-120 (2015-05-13)
ATP-binding cassette subfamily B member 1 (ABCB1) and subfamily C member 10 (ABCC10) proteins are efflux transporters that couple the energy derived from ATP hydrolysis to the translocation of toxic substances and chemotherapeutic drugs out of cells. Cabazitaxel is a
J Robin Harris
Micron (Oxford, England : 1993), 39(2), 168-176 (2007-09-07)
The negative staining technique, when used with holey carbon support films, presents superior imaging conditions than is the case when samples are adsorbed to continuous carbon films. A demonstration of this negative staining approach is presented, using ammonium molybdate in
B Shyla et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 78(1), 497-502 (2010-12-15)
A simple spectrophotometric method is developed here for the determination of phosphate present in the samples of soil, detergents, water, bone and food based on the formation of phosphomolybdate complex with the added molybdate followed by the reduction of the
Jae-Kweon Park et al.
Journal of microbiology and biotechnology, 19(9), 918-921 (2009-10-08)
In the present study, we examined the inhibitory effects of protein tyrosine phosphatase (PTPase) inhibitors, including sodium orthovanadate (SOV), ammonium molybdate (AM), and iodoacetamide (IA), on cell growth, accumulation of astaxanthin, and PTPase activity in the photosynthetic algae Haematococcus lacustris.
Maryam Asnaashari et al.
Journal of food science and technology, 52(8), 5180-5187 (2015-08-06)
Efficacy of R. fruticosus leaves extract in stabilizing sunflower oil during accelerated storage has been studied. Extracts of R. fruticosus were prepared in different solvents which methanolic extract yield with 15.43 % was higher than water and acetone ones (11.87 and
Abhisek Chatterjee et al.
Biochimie, 105, 64-75 (2014-06-28)
We cloned, overexpressed and purified Vibrio cholerae FtsZ protein for the first time. We used several complementary techniques to probe and compare the comparative assembly properties of recombinant Vibrio cholerae FtsZ (VcFtsZ) and Escherichia coli FtsZ (EcFtsZ). We observed that VcFtsZ
Suvarna Sounderajan et al.
Journal of hazardous materials, 149(1), 238-242 (2007-09-01)
Arsenic (V) is known to form heteropolyacid with ammonium molybdate in acidic aqueous solutions, which can be quantitatively extracted into certain organic solvents. In the present work, 12-molybdoarsenic acid extracted in butan-1-ol is used for quantification of As (V). Total
Yuan Lin et al.
The Journal of antibiotics, 67(9), 671-676 (2014-07-06)
Antibiotic resistance to Mycobacterium tuberculosis is a growing problem. Therefore, development of new anti-tuberculosis antibiotics is urgent for the control of tuberculosis (TB) infections. FtsZ, the homolog of eukaryotic tubulin, is a GTPase that assembles into cytokinetic Z rings essential
S Song et al.
Letters in applied microbiology, 60(6), 597-604 (2015-03-17)
Lactobacilli in the human gastrointestinal tract have beneficial effects on the health of their host. To enhance these effects, the bioactivity of lactobacilli can be fortified through exogenous dietary or pharmacological agents, such as glycoproteins. To elucidate the inductive effect
Handbook of Non-Ferrous Metal Powders: Technologies and Applications null
Ana L Gonçalves et al.
Bioresource technology, 200, 279-286 (2015-10-27)
Cultivation of microalgae and cyanobacteria has been the focus of several research studies worldwide, due to the huge biotechnological potential of these photosynthetic microorganisms. However, production of these microorganisms is still not economically viable. One possible alternative to improve the
Sayantanee Niyogi et al.
Journal of cell science, 128(12), 2363-2373 (2015-05-13)
The contractile vacuole complex (CVC) of Trypanosoma cruzi, the etiologic agent of Chagas disease, collects and expels excess water as a mechanism of regulatory volume decrease after hyposmotic stress; it also has a role in cell shrinking after hyperosmotic stress.
Claudia Jäckel et al.
BMC genomics, 16, 629-629 (2015-08-25)
The application of phages is a promising tool to reduce the number of Campylobacter along the food chain. Besides the efficacy against a broad range of strains, phages have to be safe in terms of their genomes. Thus far, no
Md Mahbubur Rahman et al.
BMC research notes, 8, 621-621 (2015-11-01)
In humans, many diseases are associated with the accumulation of free radicals. Antioxidants can scavenge free radicals and minimize their impact. Therefore, the search for naturally occurring antioxidants of plant origin is imperative. Here, we aimed to investigate the antioxidant
Li Hua et al.
Legal medicine (Tokyo, Japan), 11(2), 80-82 (2008-12-02)
A simple and rapid method was developed for the routine determination and classification of inorganic arsenic based on its clinical and forensic properties. Inorganic arsenic was isolated from urine by using copper granules, which was then made to react with
Linlin Jing et al.
BMC complementary and alternative medicine, 15, 287-287 (2015-08-19)
Rhododendron anthopogonoides Maxim, a kind of traditional Tibetan medicine, has been used to remove body heat, body detoxification, cough, asthma, stomachic and swelling, eliminate abundant phlegm and inflammatory for a long time. In the present study, the total phenols and
Lok Hang Mak et al.
Methods (San Diego, Calif.), 77-78, 63-68 (2015-03-10)
PTEN (phosphatase and tensin homologue deleted on chromosome 10) is well known as a tumour suppressor. It's PI(3,4,5)P3 lipid phosphatase activity is an important counteracting mechanism in PI 3-kinase (phosphoinositide 3-kinase) signalling. Furthermore, PTEN lies upstream of Akt kinase, a
S Ganesh et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 66(12), 2653-2658 (2012-10-31)
A simple spectrophotometric method has been developed for the determination of phosphate dissolved in soil and water. The method is based on the formation of phosphomolybdate with added ammonium molybdate followed by reduction with hydrazine in acidic medium. Orthophosphate and
Md Nasim Uddin et al.
BMC complementary and alternative medicine, 15, 195-195 (2015-06-24)
Alzheimer's disease (AD) is a progressively developing neurodegenerative disorder of the brain in the elderly people. Vanda roxburghii Rbr. root has been used traditionally in Bangladesh as tonic to brain and in the treatment of nervous system disorders including AD.
Adam Bownik et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 170, 38-49 (2015-02-24)
Ectoine (ECT) is an osmoprotectant produced by halophilic microorganisms inducing protective effects against various stressful factors. However, little is known about its influence on aquatic invertebrates subjected to hydrogen peroxide (H2O2)-a commonly used oxidative disinfectant. Therefore, the aim of our
Nagaraju Anreddy et al.
Oncotarget, 6(36), 39276-39291 (2015-10-31)
ATP-binding cassette subfamily G member 2 (ABCG2) is a member of the ABC transporter superfamily proteins, which has been implicated in the development of multidrug resistance (MDR) in cancer, apart from its physiological role to remove toxic substances out of
Gehad G Mohamed et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 65(5), 1221-1226 (2006-07-11)
Extraction spectrophotometric method has been developed for the determination of tricyclic drugs such as trazodone (TZH), amineptine (APH) and amitriptyline (ATPH) hydrochlorides in pure form and in the dosage forms coming from different Egyptian markets. The method based on the
Antioxidant and antiproliferative activities of heterofucans from the seaweed Sargassum filipendula.
Leandro Silva Costa et al.
Marine drugs, 9(6), 952-966 (2011-07-13)
Fucan is a term used to denominate a type of polysaccharide which contains substantial percentages of l-fucose and sulfate ester groups. We obtained five heterofucans from Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. These heterofucans are composed
Lama Talje et al.
FEBS letters, 588(14), 2315-2320 (2014-05-27)
The kinesin-13 family of microtubule depolymerases is a major regulator of microtubule dynamics. RNA interference-induced knockdown studies have highlighted their importance in many cell division processes including spindle assembly and chromosome segregation. Since microtubule turnovers and most mitotic events are
Principles of micro Sequential Injection Analysis in the Lab-on-Valve format and its Introduction into a Teaching Laboratory
Decuir MS, et al.
Journal of Flow Injection Analysis, 24(2), 103-103 (2007)
Spencer M Steinberg
Environmental monitoring and assessment, 185(5), 3749-3757 (2012-08-15)
A new method for determination of hydrogen peroxide (H2O2) is described. H2O2 reacts with iodide in the presence of ammonium molybdate and vanillic acid resulting in the formation of iodovanillic acid which is quantified by reversed-phase high-performance liquid chromatography (HPLC)
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