through 5. Pipette 900 µl of 0.1 M HCl into tube 1
and 750 µl of 0.1 M HCl into tubes 2-5. Add 100 µl
of the Cyclic AMP Standard to tube 1. Vortex
thoroughly. Add 250 µl of tube
Pipette 900 µl of Assay Buffer 2 into
tube 1 and 750 µl of Assay Buffer 2 into tubes 2–5.
Add 100 µl of the Cyclic AMP Standard to tube 1.
Vortex thoroughly. Add 250 µl of tube 1 to
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