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Işıl Gerçek Beşkardeş et al.
Journal of tissue engineering and regenerative medicine, 9(11), 1233-1246 (2012-12-15)
In this study, a novel scaffold fabrication method was developed by combining microwave irradiation and gas foaming. Chitosan superporous hydrogels (SPHs) and chitosan-hydroxyapatite (HA) superporous hydrogel composites (SPHCs) were prepared by using this method in the presence of crosslinking agent
Benedicta E Beck-Broichsitter et al.
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 43(6), 981-988 (2015-05-16)
The timing of application of recombinant human bone morphogenic protein-2 (rhBMP-2) may be important in determining the final outcome of engineered bone tissue. This study investigates the impact of repetitive rhBMP-2 application on hard and soft tissue morphology in endocultivation.
Mehdi Farokhi et al.
International journal of pharmaceutics, 454(1), 216-225 (2013-07-17)
To exploit the therapeutic potential of growth factors in tissue regeneration, it is necessary to design a porous scaffold in order to concurrently accommodate cells and release angiogenic factors in a controlled manner. In an attempt to address these issues
Niladri Nath Panda et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 103(5), 971-982 (2014-09-02)
This study evaluated the mechanical properties and osteogenic potential of a silk fibroin scaffold prepared from a 70:30 blend of Eri (Philosamia ricini) and Tasar (Antheraea mylitta) silk, respectively (ET scaffolds). An electrospinning process was used to prepare uniformly blended
David Pastorino et al.
Acta biomaterialia, 28, 205-214 (2015-09-20)
Characterization of the intricate pore structure of calcium phosphate cements is a key step to successfully link the structural properties of these synthetic bone grafts with their most relevant properties, such as in vitro or in vivo behaviour, drug loading
C Reilly et al.
Journal of applied microbiology, 117(6), 1798-1809 (2014-09-30)
To test the effect of 0·4% stannous fluoride (SnF2 ) glycerine-based gels on specific portions of the bacterial community in both a clinical observational study and in vitro multispecies plaque-derived (MSPD) biofilm model. Potential changes to specific portions of the
Julien Ramier et al.
Journal of materials science. Materials in medicine, 25(6), 1563-1575 (2014-03-04)
Electrospinning coupled with electrospraying provides a straightforward and robust route toward promising electrospun biocomposite scaffolds for bone tissue engineering. In this comparative investigation, four types of poly(3-hydroxybutyrate) (PHB)-based nanofibrous scaffolds were produced by electrospinning a PHB solution, a PHB/gelatin (GEL)
Giulia Pavani et al.
Blood, 124(7), 1157-1165 (2014-06-25)
Recombinant activated human factor VII (rhFVIIa) is an established hemostatic agent in hemophilia, but its mechanism of action remains unclear. Although tissue factor (TF) is its natural receptor, rhFVIIa also interacts with the endothelial protein C receptor (EPCR) through its
R Osorio et al.
Journal of dental research, 93(12), 1258-1263 (2014-09-18)
To obtain more durable adhesion to dentin, and to protect collagen fibrils of the dentin matrix from degradation, calcium- and phosphate-releasing particles have been incorporated into the dental adhesive procedure. The aim of the present study was to incorporate zinc-loaded
Stephan Benke et al.
Nature communications, 6, 6198-6198 (2015-02-06)
Pore-forming toxins are protein assemblies used by many organisms to disrupt the membranes of target cells. They are expressed as soluble monomers that assemble spontaneously into multimeric pores. However, owing to their complexity, the assembly processes have not been resolved
Weihong Qu et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 990, 15-22 (2015-04-05)
Column chromatography has been widely used as a scalable purification strategy for recombinant adeno-associated virus (rAAV) vectors. The rAAV1, 2, 4, 5, 6, 8 and 9 serotypes could be separated using affinity resins, ion exchange resins or other types of
Yoshitaka Ogita et al.
The Journal of biological chemistry, 290(33), 20245-20256 (2015-07-08)
The Ras family of small GTPases function in a wide variety of biological processes as "molecular switches" by cycling between inactive GDP-bound and active GTP-bound forms. Di-Ras1 and Di-Ras2 were originally identified as small GTPases forming a distinct subgroup of
Sayed Modinur Rahaman et al.
Indian journal of biochemistry & biophysics, 52(2), 119-124 (2015-06-30)
The role of angiotensin II in regulating Na+/K(+)-ATPase activity has been investigated in bovine pulmonary artery smooth muscle cells (BPASMCs). Our study reveals that angiotensin II inhibits the Na+/K+ATPase activity via glutathionylation of the pump with the involvement of an
Tie Liu et al.
Bone, 56(1), 110-118 (2013-06-05)
As an alternative to an autologous bone graft, deproteinized bovine bone (DBB) is widely used in the clinical dentistry. Although DBB provides an osteoconductive scaffold, it is not capable of enhancing bone regeneration because it is not osteoinductive. In order
Georgios N Belibasakis et al.
Virulence, 6(7), 704-709 (2015-08-26)
Periodontitis is an infectious inflammatory disease that destroys the tooth-supporting tissues. It is caused by the formation of subgingival biofilms on the surface of the tooth. Characteristic bacteria associated with subgingival biofilms are the Gram-negative anaerobes Porphyromonas gingivalis, Tannerella forsythia
Radosław Olkowski et al.
Journal of materials science. Materials in medicine, 26(12), 270-270 (2015-10-30)
Calcium phosphate cements (CPC) are valuable bone fillers. Recently they have been also considered as the basis for drug-, growth factors- or cells-delivery systems. Broad possibilities to manipulate CPC composition provide a unique opportunity to obtain materials with a wide
Petra Korlević et al.
BioTechniques, 59(2), 87-93 (2014-01-01)
Although great progress has been made in improving methods for generating DNA sequences from ancient biological samples, many, if not most, samples are still not amenable for analyses due to overwhelming contamination with microbial or modern human DNA. Here we
Minsung Park et al.
Colloids and surfaces. B, Biointerfaces, 130, 222-228 (2015-04-26)
Nanofibrous 2,2,6,6-tetramethylpiperidine-1-oxyl(TEMPO)-oxidized bacterial cellulose (TOBC) was used as a dispersant of hydroxyapatite (HA) nanoparticles in aqueous solution. The surfaces of TOBC nanofibers were negatively charged after the reaction with the TEMPO/NaBr/NaClO system at pH 10 and room temperature. HA nanoparticles
Taichi Inui et al.
Applied and environmental microbiology, 81(16), 5471-5476 (2015-06-07)
Carbohydrate availability shifts when bacteria attach to a surface and form biofilm. When salivary planktonic bacteria form an oral biofilm, a variety of polysaccharides and glycoproteins are the primary carbon sources; however, simple sugar availabilities are limited due to low
Chen Li et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 114, 575-578 (2013-07-03)
Eu(2+)-Yb(3+) co-doped transparent glass-ceramic containing nano-Ca5(PO4)3F (FAP) was prepared in reducing atmosphere. XRD and TEM analysis indicated that nano-FAP about 40 nm precipitated homogeneously in glass matrix after heat treatment. Confirmed by spectroscopy measurements, the crystal-like absorption and emission of
Kushagra Saran et al.
Advanced healthcare materials, 4(3), 432-445 (2014-10-10)
During minimally invasive orthopedic surgeries, surgical intervention is required at two stages; to attain hemostasis and subsequently to implant the bone graft or its substitute. There is an apparent need for a material which can simultaneously control bone bleeding and
Thomas Lyon et al.
The Journal of bone and joint surgery. American volume, 95(23), 2088-2096 (2013-12-07)
Recombinant human bone morphogenetic protein-2 (rhBMP-2) applied on an absorbable collagen sponge improves open tibial fracture-healing as an adjunct to unreamed intramedullary nail fixation. We evaluated rhBMP-2 and a new, injectable calcium phosphate matrix (CPM) formulation in acute closed tibial
Bernhard Gasselhuber et al.
Biochemistry, 54(35), 5425-5438 (2015-08-21)
Recently, it was demonstrated that bifunctional catalase-peroxidases (KatGs) are found not only in archaea and bacteria but also in lower eukaryotes. Structural studies and preliminary biochemical data of the secreted KatG from the rice pathogen Magnaporthe grisea (MagKatG2) suggested both
Ming Pei et al.
Bone, 78, 1-10 (2015-05-06)
Repairing large bone defects presents a significant challenge, especially in those people who have a limited regenerative capacity such as in osteoporotic (OP) patients. The aim of this study was to compare adipose stem cells (ASCs) from both normal (NORM)
Francisco Miguel Cardoso et al.
Journal of virology, 88(15), 8278-8296 (2014-05-16)
Influenza virus neuraminidase (NA) is an interesting target of small-molecule antiviral drugs. We isolated a set of H5N1 NA-specific single-domain antibodies (N1-VHHm) and evaluated their in vitro and in vivo antiviral potential. Two of them inhibited the NA activity and
Krzysztof Zapałowicz et al.
Neurologia i neurochirurgia polska, 47(6), 590-594 (2014-01-01)
The authors describe the use of bone cement containing calcium phosphate for vertebroplasty of the cavity in the base of odontoid process. A 23-year-old female patient was operated on by incision in lateral cervical area (anterior open access). After a
Huan Zhou et al.
Materials science & engineering. C, Materials for biological applications, 33(7), 4288-4294 (2013-08-06)
There are two interesting features of this paper. First, we report herein a novel microwave assisted technique to prepare phosphate based orthopedic cements, which do not generate any exothermicity during setting. The exothermic reactions during the setting of phosphate cements
Salwa M Maria et al.
Acta biomaterialia, 20, 140-146 (2015-04-02)
Bone resorbing osteoclasts represent an important therapeutic target for diseases associated with bone and joint destruction, such as rheumatoid arthritis, periodontitis, and osteoporosis. The quantification of osteoclast resorptive activity in vitro is widely used for screening new anti-resorptive medications. The
Bor-Shiunn Lee et al.
Scientific reports, 5, 13352-13352 (2015-08-22)
There is no topically applicable low concentration fluoride delivery device available for caries prevention. This study was aimed to assess the use of a low concentration (1450 ppm) fluoride strip as an effective fluoride delivery system against enamel demineralization. The enamel
Mohammad Mujahid Ali Khan et al.
Materials science & engineering. C, Materials for biological applications, 33(7), 4228-4235 (2013-08-06)
Calcium-barium phosphate (CBP) composite membrane with 25% polystyrene was prepared by co-precipitation method. Scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transformed infrared (FTIR), and Thermogravimetric analysis (TGA) were used to characterize the membrane. The membrane was found to be
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