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purification from hybridoma cells ACTIVITY
Western blotting
Serial dilutions, 3-fold, of nuclear extracts of HEK 293 cells were run on 4-12%
NuPAGE gels and transferred to Immobilon™-P membranes (Millipore
Uptake is not dependent upon mitochondrial membrane potential, good for measuring mass
RHOD 123 507 529 Indicator of mitochondrial membrane potential and ATP production, fast but can be self-quenching
N3038 79923 NANOG M NP_079141.2 y n/d n/d
732 b-Tubulin I+II T8535 81027 TUBB1 M NP_110400.1 y y y
507 NG2 N8912 81651 Cspg4 M NP_112284.1 n/d n/d y
650 S-100 S2644 81778 S100a10 P NP_112376.1 y n/d y
519
N3038 79923 NANOG M NP_079141.2 y n/d n/d
732 b-Tubulin I+II T8535 81027 TUBB1 M NP_110400.1 y y y
507 NG2 N8912 81651 Cspg4 M NP_112284.1 n/d n/d y
650 S-100 S2644 81778 S100a10 P NP_112376.1 y n/d y
519
N3038 79923 NANOG M NP_079141.2 y n/d n/d
732 b-Tubulin I+II T8535 81027 TUBB1 M NP_110400.1 y y y
507 NG2 N8912 81651 Cspg4 M NP_112284.1 n/d n/d y
650 S-100 S2644 81778 S100a10 P NP_112376.1 y n/d y
519
Sh Xn
Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) H M R WB APur Rb IgG 200 µL 07-507
cAMP
Cyclic AMP (cAMP, adenosine 3´, 5´-cyclic monophosphate) is a nucleotide that acts as a key second messenger in
y y
293 GADD 153 (CHOP-10) G6916 P y y n/d
294 GAP1IP4BP G6666 M y n/d n/d
295 GAPDH G8795 M y y y
296 GATA-1 G0290 P y n/d n/d
297 Gelsolin G4896 M y n/d n/d
298 Gemin 2 G6669 M y y
/d
506 NAK (NFκB-Activating Kinase) N2661 29110, 362887 Tbk1_predicted,TBK1 NP_037386.1, M y n/d y
507 NG2 N8912 81651 Cspg4 NP_112284.1 M n/d n/d y
508 Nicastrin N1660 23385 NCSTN NP_056146.1 P y n/d n
/d
506 NAK (NFκB-Activating Kinase) N2661 29110, 362887 Tbk1_predicted,TBK1 NP_037386.1, M y n/d y
507 NG2 N8912 81651 Cspg4 NP_112284.1 M n/d n/d y
508 Nicastrin N1660 23385 NCSTN NP_056146.1 P y n/d n