was
exposed to 300 °C for a total of 300 minutes.
FID Bleed
The observed FID bleed levels from the 1st ‘300 °C’ and the 15th
‘300 °C’ run for all columns are displayed in Figure 1. All five
the solution per well and
allow the samples to incubate for 1–2 hours
at a temperature range of 18–30°C.
3. Wash the wells three times, 300 µl per well,
with PBS or TBS + 0.05% Tween-20.
4. Incubate
ProClin™ 300 preservative in a specific
product before its use.10
-3
10-2
10-1
100
101
102
0 15 30 45
*CMIT = Chloromethyl isothiazolin (primary active ingredient in ProClin™ 300 preservative
Table 1. Maximum Temperature Limits *
Column Isothermal Programmed
PEG 1 280 °C 280 °C
PEG 2 260 °C 270 °C
PEG 3 250 °C 260 °C
PEG 4 250 °C 260 °C
PEG 5 280 °C 300 °C
SLB-IL60 300 °C 300
the samples to incubate for 1–2 hours at a
temperature within the range of 18–30 °C. Include
blank and control wells as appropriate.
3. Wash the wells three times, 300 µl per well, with
PBS or TBS
the samples to incubate for 1–2 hours at a
temperature within the range of 18–30 °C. Include
blank and control wells as appropriate.
3. Wash the wells three times, 300 µl per well, with
PBS or TBS
the samples to incubate for 1–2 hours at a
temperature within the range of 18–30 °C. Include
blank and control wells as appropriate.
3. Wash the wells three times, 300 µl per well, with
PBS or TBS
peak 35)
Column Isothermal Programmed
PEG 1 280 °C 280 °C
PEG 2 260 °C 270 °C
PEG 3 250 °C 260 °C
PEG 4 250 °C 260 °C
PEG 5 280 °C 300 °C
SLB-IL60 300 °C 300 °C
Description Cat. No.
SLB-IL60, 15