31550

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Actomyosin mediates gravisensing and early transduction events in reoriented cut snapdragon spikes.

Zhaoqi Zhang et al.

Journal of plant physiology, 168(11), 1176-1183 (2011-03-11)

We investigated the involvement of the actomyosin network in the early events of the gravitropic response of cut snapdragon (Antirrhinum majus L.) spikes. The effects of the actin-modulating drug, cytochalasin D (CD) and/or the myosin inhibitor, 2,3-butanedione-2-monoxime (BDM) on amyloplast

Both actin and myosin inhibitors affect spindle architecture in PtK1 cells: does an actomyosin system contribute to mitotic spindle forces by regulating attachment and movements of chromosomes in mammalian cells?

Judith A Snyder et al.

Protoplasma, 240(1-4), 57-68 (2010-01-22)

Immunocytochemical techniques are used to analyze the effects of both an actin and myosin inhibitor on spindle architecture in PtK(1) cells to understand why both these inhibitors slow or block chromosome motion and detach chromosomes. Cytochalasin J, an actin inhibitor

Increased passive stiffness of cardiomyocytes in the transverse direction and residual actin and myosin cross-bridge formation in hypertrophied rat hearts induced by chronic β-adrenergic stimulation.

Wakako Sumita Yoshikawa et al.

Circulation journal : official journal of the Japanese Circulation Society, 77(3), 741-748 (2012-12-12)

Left ventricular (LV) hypertrophy is often present in patients with diastolic heart failure. However, stiffness of hypertrophied cardiomyocytes in the transverse direction has not been fully elucidated. The aim of this study was to assess passive cardiomyocyte stiffness of hypertrophied

Multiple Effects of 2, 3-Butanedione Monoxime.

Sellin LC and McArdle JJ/

Pharmacology & Toxicology, 74 (4-5), 305-313 (1994)

Normal interstitial flow is critical for developmental lymphangiogenesis in the zebrafish.

Mikah Coffindaffer-Wilson et al.

Lymphatic research and biology, 9(3), 151-158 (2011-11-10)

The lymphatic system plays a critical role in the body's fluid and protein homeostasis, immune regulation, and dietary fat absorption. One of the major pathologies of the lymphatic system is primary lymphedema, which occurs in approximately 0.6% of live births

Mouse intact cardiac myocyte mechanics: cross-bridge and titin-based stress in unactivated cells.

Nicholas M P King et al.

The Journal of general physiology, 137(1), 81-91 (2010-12-29)

A carbon fiber-based cell attachment and force measurement system was used to measure the diastolic stress-sarcomere length (SL) relation of mouse intact cardiomyocytes, before and after the addition of actomyosin inhibitors (2,3-butanedione monoxime [BDM] or blebbistatin). Stress was measured during

Modifications of mechanoelectric feedback induced by 2,3-butanedione monoxime and Blebbistatin in Langendorff-perfused rabbit hearts.

L Brines et al.

Acta physiologica (Oxford, England), 206(1), 29-41 (2012-04-14)

Myocardial stretching is an arrhythmogenic factor. Optical techniques and mechanical uncouplers are used to study the mechanoelectric feedback. The aim of this study is to determine whether the mechanical uncouplers 2,3-butanedione monoxime and Blebbistatin hinder or modify the electrophysiological effects

Actin Polymerization Mediated by AtFH5 Directs the Polarity Establishment and Vesicle Trafficking for Pollen Germination in Arabidopsis.

Chang Liu et al.

Molecular plant, 11(11), 1389-1399 (2018-10-09)

The process of pollen germination is crucial for flowering plant reproduction, but the mechanisms through which pollen grains establish polarity and select germination sites are not well understood. In this study, we report that a formin family protein, AtFH5, is localized

Phototropin-dependent biased relocalization of cp-actin filaments can be induced even when chloroplast movement is inhibited.

Noboru Yamada et al.

Plant signaling & behavior, 6(11), 1651-1653 (2011-11-08)

In a recent publication using an actin-visualized line of Arabidopsis (Ichikawa et al. 2011, ref. 11), we reported a detailed analysis with higher time resolution on the dynamics of chloroplast actin filaments (cp-actin filaments) during chloroplast avoidance movement and demonstrated

The myosin ATPase inhibitor, 2,3-butanedione 2-monoxime, prevents protein secretion by the basidiomycete Coprinopsis cinerea.

Kohsuke Hashimoto et al.

Biotechnology letters, 33(4), 769-775 (2010-12-18)

The plant-saprophytic basidiomycete, Coprinopsis cinerea, produces and secretes various cellulases during cellulose degradation as the main extracellular proteins. Although enzymatic characterization of such cellulases has been frequently reported, the mechanism of their secretion remains unclear. This study focused on myosins

Inhibitors of myosin, but not actin, alter transport through Tradescantia plasmodesmata.

Janine E Radford et al.

Protoplasma, 248(1), 205-216 (2010-11-30)

Actin and myosin are components of plasmodesmata, the cytoplasmic channels between plant cells, but their role in regulating these channels is unclear. Here, we investigated the role of myosin in regulating plasmodesmata in a well-studied, simple system comprising single filaments

Does 2,3-butanedione monoxime inhibit nonmuscle myosin?

Arthur Forer et al.

Protoplasma, 225(1-2), 1-4 (2005-05-04)

BDM (2,3-butanedione monoxime) has been used extensively to inhibit nonmuscle myosin. However, recent articles raise the question of what BDM actually does, because of experiments in which BDM did not affect the actin-activated ATPase of nonmuscle myosins. We describe results

Reactive skin decontamination lotion (RSDL) for the decontamination of chemical warfare agent (CWA) dermal exposure.

M D Schwartz et al.

Current pharmaceutical biotechnology, 13(10), 1971-1979 (2012-02-23)

Rapid decontamination of the skin is the single most important action to prevent dermal absorption of chemical contaminants in persons exposed to chemical warfare agents (CWA) and toxic industrial chemicals (TICs) as a result of accidental or intentional release. Chemicals

Monitoring the structural behavior of troponin and myoplasmic free Ca2+ concentration during twitch of frog skeletal muscle.

Tatsuhito Matsuo et al.

Biophysical journal, 99(1), 193-200 (2010-07-27)

The interaction of troponin molecules on the thin filament with Ca(2+) plays a key role in regulating muscle contraction. To characterize the structural changes of troponin caused by Ca(2+) and crossbridge formation, we recorded the small-angle x-ray intensity and the

Cutaneous challenge with chemical warfare agents in the SKH-1 hairless mouse (II): effects of some currently used skin decontaminants (RSDL and Fuller's earth) against liquid sulphur mustard and VX exposure.

L Taysse et al.

Human & experimental toxicology, 30(6), 491-498 (2010-06-11)

Using the hairless mouse screening model presented in the companion paper(1) the aim of this study was to assess two skin decontaminating systems: Fuller's earth (FE) and Reactive Skin Decontamination Lotion (RSDL) against two extremely toxic chemical warfare agents that

2, 3-Butanedione monoxime (BDM) as a myosin inhibitor.

Ostap EM.

Journal of Muscle Research and Cell Motility, 23 (4), 305-308 (2002)

The role of dynamic instability and wavelength in arrhythmia maintenance as revealed by panoramic imaging with blebbistatin vs. 2,3-butanedione monoxime.

Qing Lou et al.

American journal of physiology. Heart and circulatory physiology, 302(1), H262-H269 (2011-11-01)

Unlike other excitation-contraction uncouplers, blebbistatin has few electrophysiological side effects and has gained increasing acceptance as an excitation-contraction uncoupler in optical mapping experiments. However, the possible role of blebbistatin in ventricular arrhythmia has hitherto been unknown. Furthermore, experiments with blebbistatin

Inhibition of contractile activity during postconditioning enhances cardioprotection by restoring sarcolemmal dystrophin through phosphatidylinositol 3-kinase.

Akira Moriguchi et al.

Circulation journal : official journal of the Japanese Circulation Society, 74(11), 2393-2402 (2010-09-30)

Although ischemic postconditioning (IPost) confers cardioprotection by protecting the mitochondria though the activation of phosphatidylinositol 3-kinase (PI3K), a potential drawback of IPost is impairment of aerobic ATP generation during reperfusion by repeated ischemia. This decrease in ATP might inhibit the

Effect of nonuniform muscle contraction on sustainability and frequency of triggered arrhythmias in rat cardiac muscle.

Masahito Miura et al.

Circulation, 121(25), 2711-2717 (2010-06-16)

Arrhythmias are benign or lethal, depending on their sustainability and frequency. To determine why lethal arrhythmias are prone to occur in diseased hearts, usually characterized by nonuniform muscle contraction, we investigated the effect of nonuniformity on sustainability and frequency of

[The effect of myosin ATPase inhibition on metabolic and functional recovery of isolated rat heart after global ischemia].

O I Pisarenko et al.

Biomeditsinskaia khimiia, 55(4), 451-461 (2009-12-17)

The effect of myosin ATPase inhibitor, 2,3-butanedione monoxime (BDM; used in the range of concentrations 1.25-10.0 mM), on recovery of functions of isolated rat heart subjected to normothermic (37 degrees C) total ischemia for 35 min has been investigated. BDM

Elastic proteins in the flight muscle of Manduca sexta.

Chen-Ching Yuan et al.

Archives of biochemistry and biophysics, 568, 16-27 (2015-01-21)

The flight muscles (DLM1) of the Hawkmoth, Manduca sexta are synchronous, requiring a neural spike for each contraction. Stress/strain curves of skinned DLM1 showed hysteresis indicating the presence of titin-like elastic proteins. Projectin and kettin are titin-like proteins previously identified

Oximes as spectrophotometric reagents-a review.

R B Singh et al.

Talanta, 26(6), 425-444 (1979-06-01)

The uses of oximes as spectrophotometric reagents since 1953 are reviewed.

Cytoskeletal proteins: hidden targets of cytoprotective signaling?

Tetsuji Miura

Circulation journal : official journal of the Japanese Circulation Society, 74(11), 2295-2296 (2010-10-22)

Calcium sensitisation impairs diastolic relaxation in post-ischaemic myocardium: implications for the use of Ca(2+) sensitising inotropes after cardiac surgery.

Yeong-Hoon Choi et al.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 37(2), 376-383 (2009-07-21)

Calcium sensitising inotropes are increasingly being used in cardiac surgical patients. Theoretically, increasing contractile protein sensitivity to Ca(2+) prevents the Ca(2+) elevation associated arrhythmogenicity and potentiates the inotropic effect of catecholamines. On the other hand, we hypothesised that Ca(2+) sensitisation

Enhanced Ca2+ binding of cardiac troponin reduces sarcomere length dependence of contractile activation independently of strong crossbridges.

F Steven Korte et al.

American journal of physiology. Heart and circulatory physiology, 303(7), H863-H870 (2012-08-07)

Calcium sensitivity of the force-pCa relationship depends strongly on sarcomere length (SL) in cardiac muscle and is considered to be the cellular basis of the Frank-Starling law of the heart. SL dependence may involve changes in myofilament lattice spacing and/or

Treatment with tertiary oximes prevents seizures and improves survival following sarin intoxication.

Tsung-Ming Shih et al.

Journal of molecular neuroscience : MN, 40(1-2), 63-69 (2009-08-15)

The capability of the tertiary oximes, monoisonitrosoacetone (MINA) and diacetylmonoxime (DAM), to reactivate acetylcholinesterase (AChE) inhibited by sarin (GB) in the blood, brain, and peripheral tissues of guinea pigs was compared with that of the quaternary oximes 2-PAM, HLö7, and

Arginase I levels are decreased in the plasma of pediatric patients with atopic dermatitis.

Victoria Dimitriades et al.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 113(3), 271-275 (2014-07-17)

Serum arginase levels have been shown to be elevated in conditions, such as trauma, cancer, chronic wounds, pregnancy, and diabetes. This also has been found to be true in atopic diseases, such as asthma and allergic rhinitis. To study arginase

W.B. Guenther

Analytical Letters, 12A, 1305-1305 (1979)

Carbamylation of immunoglobulin abrogates activation of the classical complement pathway.

Catalin Koro et al.

European journal of immunology, 44(11), 3403-3412 (2014-08-19)

Post-translational modifications of proteins significantly affect their structure and function. The carbamylation of positively charged lysine residues to form neutral homoitrulline occurs primarily under inflammatory conditions through myeloperoxidase-dependent cyanate (CNO-) formation. We analyzed the pattern of human IgG1 carbamylation under

Second-derivative spectrometric determination of urea in milk using the diacetyl monoxime reagent.

Steven A Nyanzi et al.

Journal of AOAC International, 93(2), 485-491 (2010-05-20)

The application of derivative spectrometry to resolve overlapping spectra and improve the sensitivity and selectivity of the colorimetric determination of urea in milk using diacetyl monoxime is presented. With first-derivative (1D) spectrometry, the lambda(max) of the colored complex was established

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