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31550
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Janine E Radford et al.
Protoplasma, 248(1), 205-216 (2010-11-30)
Actin and myosin are components of plasmodesmata, the cytoplasmic channels between plant cells, but their role in regulating these channels is unclear. Here, we investigated the role of myosin in regulating plasmodesmata in a well-studied, simple system comprising single filaments
Arthur Forer et al.
Protoplasma, 225(1-2), 1-4 (2005-05-04)
BDM (2,3-butanedione monoxime) has been used extensively to inhibit nonmuscle myosin. However, recent articles raise the question of what BDM actually does, because of experiments in which BDM did not affect the actin-activated ATPase of nonmuscle myosins. We describe results
Kohsuke Hashimoto et al.
Biotechnology letters, 33(4), 769-775 (2010-12-18)
The plant-saprophytic basidiomycete, Coprinopsis cinerea, produces and secretes various cellulases during cellulose degradation as the main extracellular proteins. Although enzymatic characterization of such cellulases has been frequently reported, the mechanism of their secretion remains unclear. This study focused on myosins
Tatsuhito Matsuo et al.
Biophysical journal, 99(1), 193-200 (2010-07-27)
The interaction of troponin molecules on the thin filament with Ca(2+) plays a key role in regulating muscle contraction. To characterize the structural changes of troponin caused by Ca(2+) and crossbridge formation, we recorded the small-angle x-ray intensity and the
2, 3-Butanedione monoxime (BDM) as a myosin inhibitor.
Ostap EM.
Journal of Muscle Research and Cell Motility, 23 (4), 305-308 (2002)
M D Schwartz et al.
Current pharmaceutical biotechnology, 13(10), 1971-1979 (2012-02-23)
Rapid decontamination of the skin is the single most important action to prevent dermal absorption of chemical contaminants in persons exposed to chemical warfare agents (CWA) and toxic industrial chemicals (TICs) as a result of accidental or intentional release. Chemicals
L Taysse et al.
Human & experimental toxicology, 30(6), 491-498 (2010-06-11)
Using the hairless mouse screening model presented in the companion paper(1) the aim of this study was to assess two skin decontaminating systems: Fuller's earth (FE) and Reactive Skin Decontamination Lotion (RSDL) against two extremely toxic chemical warfare agents that
L Brines et al.
Acta physiologica (Oxford, England), 206(1), 29-41 (2012-04-14)
Myocardial stretching is an arrhythmogenic factor. Optical techniques and mechanical uncouplers are used to study the mechanoelectric feedback. The aim of this study is to determine whether the mechanical uncouplers 2,3-butanedione monoxime and Blebbistatin hinder or modify the electrophysiological effects
Chang Liu et al.
Molecular plant, 11(11), 1389-1399 (2018-10-09)
The process of pollen germination is crucial for flowering plant reproduction, but the mechanisms through which pollen grains establish polarity and select germination sites are not well understood. In this study, we report that a formin family protein, AtFH5, is localized
Noboru Yamada et al.
Plant signaling & behavior, 6(11), 1651-1653 (2011-11-08)
In a recent publication using an actin-visualized line of Arabidopsis (Ichikawa et al. 2011, ref. 11), we reported a detailed analysis with higher time resolution on the dynamics of chloroplast actin filaments (cp-actin filaments) during chloroplast avoidance movement and demonstrated
Zhaoqi Zhang et al.
Journal of plant physiology, 168(11), 1176-1183 (2011-03-11)
We investigated the involvement of the actomyosin network in the early events of the gravitropic response of cut snapdragon (Antirrhinum majus L.) spikes. The effects of the actin-modulating drug, cytochalasin D (CD) and/or the myosin inhibitor, 2,3-butanedione-2-monoxime (BDM) on amyloplast
Multiple Effects of 2, 3-Butanedione Monoxime.
Sellin LC and McArdle JJ/
Pharmacology & Toxicology, 74 (4-5), 305-313 (1994)
Wakako Sumita Yoshikawa et al.
Circulation journal : official journal of the Japanese Circulation Society, 77(3), 741-748 (2012-12-12)
Left ventricular (LV) hypertrophy is often present in patients with diastolic heart failure. However, stiffness of hypertrophied cardiomyocytes in the transverse direction has not been fully elucidated. The aim of this study was to assess passive cardiomyocyte stiffness of hypertrophied
Judith A Snyder et al.
Protoplasma, 240(1-4), 57-68 (2010-01-22)
Immunocytochemical techniques are used to analyze the effects of both an actin and myosin inhibitor on spindle architecture in PtK(1) cells to understand why both these inhibitors slow or block chromosome motion and detach chromosomes. Cytochalasin J, an actin inhibitor
Mikah Coffindaffer-Wilson et al.
Lymphatic research and biology, 9(3), 151-158 (2011-11-10)
The lymphatic system plays a critical role in the body's fluid and protein homeostasis, immune regulation, and dietary fat absorption. One of the major pathologies of the lymphatic system is primary lymphedema, which occurs in approximately 0.6% of live births
Nicholas M P King et al.
The Journal of general physiology, 137(1), 81-91 (2010-12-29)
A carbon fiber-based cell attachment and force measurement system was used to measure the diastolic stress-sarcomere length (SL) relation of mouse intact cardiomyocytes, before and after the addition of actomyosin inhibitors (2,3-butanedione monoxime [BDM] or blebbistatin). Stress was measured during
Maozhong Zheng et al.
Planta, 231(4), 779-791 (2009-12-25)
Strategic control of mitochondrial movements and cellular distribution is essential for correct cell function and survival. However, despite being a vital process, mitochondrial movement in plant cells is a poorly documented phenomenon. To investigate the roles of actin filaments and
Aline Vieille-Petit et al.
Skin pharmacology and physiology, 28(2), 57-64 (2014-09-18)
Percutaneous penetration of urea in vivo in man has been documented. If urea can penetrate the skin, it may also move laterally. Lateral spreading of topical substances leads to unpredictable penetration dynamics and increased skin surface area exposure. The ability
L C Sellin et al.
Pharmacology & toxicology, 74(6), 305-313 (1994-06-01)
2,3-Butanedione monoxime, also known as diacetyl monoxime, is a nucleophilic agent which dephosphorylates acetylcholinesterase poisoned with organophosphates. This "chemical phosphatase" activity stimulated studies of the effect of 2,3-butanedione monoxime on phosphorylation-dependent cellular processes. As a result of these studies, we
Improvements on the Prescott-Jones method for the colorimetric analysis of ureido compounds.
D B Shindler et al.
Analytical biochemistry, 97(2), 421-422 (1979-09-01)
W.B. Guenther
Analytical Letters, 12A, 1305-1305 (1979)
Catalin Koro et al.
European journal of immunology, 44(11), 3403-3412 (2014-08-19)
Post-translational modifications of proteins significantly affect their structure and function. The carbamylation of positively charged lysine residues to form neutral homoitrulline occurs primarily under inflammatory conditions through myeloperoxidase-dependent cyanate (CNO-) formation. We analyzed the pattern of human IgG1 carbamylation under
Emilia Entcheva et al.
Integrative biology : quantitative biosciences from nano to macro, 1(2), 212-219 (2009-12-22)
This study tests the hypothesis that the cell cytoskeletal (CSK) network can rearrange from geodesic dome type structures to stress fibers in response to microenvironmental cues. The CSK geodesic domes are highly organized actin microarchitectures within the cell, consisting of
Nelson Escobales et al.
Journal of molecular and cellular cardiology, 77, 136-146 (2014-12-03)
Mitochondria-generated reactive oxygen species (ROS) play a crucial role in the pathogenesis of aging and age-associated diseases. In this study, we evaluated the effects of XJB-5-131 (XJB), a mitochondria-targeted ROS and electron scavenger, on cardiac resistance to ischemia-reperfusion (IR)-induced oxidative
Treatment with tertiary oximes prevents seizures and improves survival following sarin intoxication.
Tsung-Ming Shih et al.
Journal of molecular neuroscience : MN, 40(1-2), 63-69 (2009-08-15)
The capability of the tertiary oximes, monoisonitrosoacetone (MINA) and diacetylmonoxime (DAM), to reactivate acetylcholinesterase (AChE) inhibited by sarin (GB) in the blood, brain, and peripheral tissues of guinea pigs was compared with that of the quaternary oximes 2-PAM, HLö7, and
V S Kuz'min et al.
Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova, 98(9), 1119-1130 (2013-01-09)
In this study, conduction of excitation in the rat left atrial and pulmonary veins myocardium was assessed by the optical mapping technique. Rat atrial myocardium and pulmonary vein myocardium demonstrated weak differences in the activation time and conduction velocity. Maximal
Steven A Nyanzi et al.
Journal of AOAC International, 93(2), 485-491 (2010-05-20)
The application of derivative spectrometry to resolve overlapping spectra and improve the sensitivity and selectivity of the colorimetric determination of urea in milk using diacetyl monoxime is presented. With first-derivative (1D) spectrometry, the lambda(max) of the colored complex was established
Chuanchau J Jou et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 25(4-5), 419-424 (2010-03-25)
The zebrafish is an emerging model system for the study of cardiac electrophysiology and human arrhythmias. High resolution imaging techniques are powerful tools for the study of zebrafish cardiac electrophysiology, but these methods require the complete absence of cardiac contraction.
Victoria Dimitriades et al.
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 113(3), 271-275 (2014-07-17)
Serum arginase levels have been shown to be elevated in conditions, such as trauma, cancer, chronic wounds, pregnancy, and diabetes. This also has been found to be true in atopic diseases, such as asthma and allergic rhinitis. To study arginase
Yeong-Hoon Choi et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 37(2), 376-383 (2009-07-21)
Calcium sensitising inotropes are increasingly being used in cardiac surgical patients. Theoretically, increasing contractile protein sensitivity to Ca(2+) prevents the Ca(2+) elevation associated arrhythmogenicity and potentiates the inotropic effect of catecholamines. On the other hand, we hypothesised that Ca(2+) sensitisation
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