1 min. Transfer column to new collection tube.
10. Add second 500 µL diluted Wash Solution to column.
Spin 1 min.
Elute purified DNA
11. Transfer column to new collection tube.
12. Add 100
Preparation Instructions
This product is soluble in water (330 mg/ml), yielding a
clear, colorless solution.
References
1. Good, N. E., et al, Hydrogen ion buffers for
biological research
working dilutions by titration.
References
1. Goldschmidt-Clermont, P.J., et al., J. Cell Biol.,
113, 1081-1089 (1991).
2. Honore, B., et al., FEBS Lett., 330, 151-155
(1993).
3. Braun, A., et al.
a signal peptide from human CD33 (Met 1–Ala 16),
amino acids 20-232 of mouse noggin, and a
polypeptide linker, fused to the Fc region of human IgG
(Pro 100–Lys 330). Mouse Noggin/Fc has Leu 20 at
the amino
Sec. B. Suppl., 217, 1 (1971)
5. Present Status and Future Work, WHO Sponsored collaborative study, Chicago (Oct. 1967)
6. J.H. Müller, J. Hinton, Proc. Soc. Exptl. Biol. 48, 330 (1941)
7. G.M. Eliopoulus
recommended. Do not store in a frost-free freezer.
References
1. van Kooten, C., and Banchereau, J., Curr. Opin.
Immunol., 9, 330-337 (1997).
2. van Kooten, C., and Banchereau, J., J. Leukoc.
319-
330 (1998).
6. Avanzi, G., et al., Selective growth response to IL-3
of human leukemic cell line with megakaryoblastic
features. Br. J. Haematol., 69, 359-366 (1988).
BKR,KAA,MAM 06/10-1
dilution samples should be discarded if not used within
12 hours.
Product Profile
By immunoblotting, a working antibody concentration of
0.5-1 µg/ml is recommended using a whole cell extract
of
8, 325-330 (1996)
4. Goedecke, W., et al., Mre11 and Ku70 interact in
somatic cells, but are differentially expressed in
early meiosis. Nature Genet., 23, 194-198 (1999).
AH 12/07/01
affiliates. All Rights Reserved.
Page 2 of 2
References:
1. J.H. Müller, J. Hinton, Proc. Soc. Exptl. Biol. 48, 330 (1941)
2. A. Bauer et al, Am. J. CIin. Path.
polyacrylamide gel electrophoresis of chloroplast
thylakoid membrane proteins. Anal. Biochem.,
171, 330 (1988).
6. Luche, S., et al., Evaluation of nonionic and
zwitterionic detergents as membrane protein
(2008).
6. Moser, M. et al., Nat. Med., 14, 325-330 (2008).
7. Moser, M. et al., Nat. Med., 15, 300-305 (2009).
VS,ST,TD,KAA,PHC,MAM 04/19-1
2019 Sigma-Aldrich Co. LLC. All rights reserved
solution. Final
osmolality should be at 290 – 330 mOsmol/kg. Adjust
with NaCl if necessary.
• Add cell culture grade water to reach a final volume
of 1 L.
• Sterile filter using a sterilizing-grade
References
1. Uribe, R., and Jay, D., Mol. Biol. Rep., 36,
121-125 (2009).
2. Ohtsuka, T. et al., J. Cell Biol., 143,
1227-1238 (1998).
3. Wang, W. et al., Biochem. Biophys. Res.
Commun., 330, 1127-1131
cleavage sites, an autoactivation site at
315/316 and a caspase-3 cleavage site between
residues 330/331. Autoactivation of pro-caspase-9 by
cleavage at site 315/316 occurs as a result of Apaf-
mediated
splenocytes from BALB/c mice
immunized with a synthetic peptide corresponding to
amino acids 216-330 of mouse TIN-2 (GeneID: 28113),
conjugated to KLH. The corresponding sequence
differs by two amino
10, 649-664
(1999).
5. Ball, J. R., and Ulman, K. S., Chromosoma, 114,
319-330 (2005).
GG,DXP,PHC 08/08-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc
recommend
determining the optimal working dilutions by titration.
References
1. Senin, I.I., et al., J. Mol. Biol., 330, 409-418 (2003).
2. Günhan, E., et al., J. Neurosci., 23, 1383-1389
(2003).
working dilutions by titration.
References
1. Goldschmidt-Clermont, P.J., et al., J. Cell Biol.,
113, 1081-1089 (1991).
2. Honore, B., et al., FEBS Lett., 330, 151-155 (1993).
3. Braun, A., et al., Gene