Physiology, 354,
273-286 (1984).
7. Data for Biochemical Research, 3rd ed., Dawson,
R. M. C., et al., Oxford University Press (New
York, NY: 1986), p. 348.
CMH/RXR 11/03
Sigma
Methods in Microbiology, Vol. 38,
Academic Press (London, UK), pp. 325-348 (2011).
13. Deodhar, D. et al., J. Glob. Infect. Dis., 7(3), 97-102
(2015).
Ficoll is a registered trademark of GE Healthcare
µL per well of 1X Anti-Rabbit IgG, HRP-conjugate.
6. Incubate the plate(s) at 37°C for 60 minutes.
7. Wash the plate(s) two times with 1X TBS-T and twice with TBS. Empty wells by inverting
the
eukaryotes. Members of this family contain a 250 amino
acid core domain that shares about 25-60% sequence
identity.7 The mammalian Sir2 gene family is
comprised of seven members which are designated
SIRT1
Methods in
Microbiology, Vol. 38, Academic Press (London,
UK), pp. 325-348 (2011).
25. Deodhar, D. et al., J. Glob. Infect. Dis., 7(3),
97-102 (2015).
Notice
We provide information and advice
Methylated DNA Capture
7. Dilute Capture Antibody 1:1000 in 1× Wash Buffer.
8. Add 50 µL of diluted Capture Antibody to each
well.
9. Cover and incubate at room temperature for
60 minutes.
10. Remove
rc
en
t
M
ax
im
u
m
R
FU
0
20
40
60
80
100
Sample 1
Sample 2
Sample 3
Sample 4
Sample 5
Sample 6
Sample 7
Sample 8
Control
Duplicate Control
Duplicate 3
Duplicate 6
Antibodies: A Laboratory
Manual. Cold Spring Harbor Laboratory Press
(Cold Spring Harbor, NY), pp. 346-348 (1988).
4. Bergmeyer, H.-U. et al., Methods of Enzymatic
Analysis, 2nd ed. (Bergmeyer, H.-U.
temperature (18–25 °C) for
60 minutes.
6. Remove liquid from all wells. Wash wells three
times with 300 µL of 1x wash buffer. Blot on
absorbent paper or paper towel.
7. Dispense 100 µL of TMB
. Mod Pathol. 21(3):231-7.
4. Heinonen, H. et al. (2008) Deciphering downstream gene targets of PI3K/mTOR/p70S6K
pathway in breast cancer. BMC Genomics. 2008 Jul 24; 9:348.
5. Jiang, B.H. et al.
Fluorogenic and
chromogenic substrates used in bacterial diagnostics. Micro-
biological Review 55, 335–348.
McFeters, G.A., Broadaway, S.C., Pyle, B.H., Pickett, M. and
Egozy, Y. (1995) Comparative performance
94 °C for 2 minutes to denature•
35 cycles of:•
94 °C for 1 minute•
60 °C for 1 minute•
72 °C for 1 minute•
72 °C for 7 minutes•
4 °C hold•
Resolve 5 μl of resulting amplicons on a
αβ-Smooth Muscle A5228 59 ACTA2 M NP_001135417.1, NP_001604.1, y y y
6 β-Actin A1978 60 ACTB M NP_001092.1 y y y
7 β-Actin A2228 60 ACTB M NP_001092.1 y y y
8 a-Actinin A5044 87 ACTN1 M NP_001093.1 y y n/d
555
αβ-Smooth Muscle A5228 59 ACTA2 M NP_001135417.1, NP_001604.1, y y y
6 β-Actin A1978 60 ACTB M NP_001092.1 y y y
7 β-Actin A2228 60 ACTB M NP_001092.1 y y y
8 a-Actinin A5044 87 ACTN1 M NP_001093.1 y y n/d
555