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Diana G Eng et al.
Kidney international, 88(5), 999-1012 (2015-05-21)
As adult podocytes cannot adequately proliferate following depletion in disease states, there has been interest in the potential role of progenitors in podocyte repair and regeneration. To determine whether parietal epithelial cells (PECs) can serve as adult podocyte progenitors following
Jinkun Zhu et al.
Talanta, 93, 428-432 (2012-04-10)
As a kinetic process, chemiluminescence (CL) met great challenges while it was used in the detection methods coupled with capillary electrophoresis (CE). In this investigation, a newly recorded ultra-fast CL reaction of luminol-KIO(4)-K(3)Fe(CN)(6) was observed to be completed in 0.65
Swati B Jadhav et al.
Carbohydrate polymers, 92(2), 1724-1729 (2013-02-13)
Nine polysaccharides differing in structure and chemical nature were screened for their ability to conjugate with α-amylase by covalent binding for enhancing the thermal and pH stability of α-amylase. Among these polysaccharides, agar, dextran, pectin and xanthan showed better results
Sevim Yildiz Arslan et al.
Endocrinology, 156(4), 1602-1609 (2015-01-31)
The endocervix has both anatomical and biological functions that participate in the delicate balance between tolerance necessary for conception and protection from pathogens. Our goal was to develop a robust 3-dimensional (3D) endocervix model that was a reliable representation of
Dmitriy Berillo et al.
Macromolecular bioscience, 12(8), 1090-1099 (2012-06-08)
Macroporous scaffolds composed of chitosan and using oxidized dextran as a crosslinker are produced through cryogelation. Introducing gelatin as a third component into the structure results in the formation of mesopores in the pore walls, which are not seen if
Ming-Jie Han et al.
Chemical communications (Cambridge, England), 48(90), 11079-11081 (2012-10-09)
An efficient aqueous Wittig reaction was enabled on protein bioconjugation for the first time. By investigating the reaction on small molecules, peptides, and proteins, a site-specific reaction targeting "aldehyde tag" was presented. A variety of functional groups could be introduced
Jae Ho Choi et al.
Journal of agricultural and food chemistry, 63(5), 1468-1476 (2015-01-16)
We investigated the inhibitory effects of Platycodon grandiflorum root-derived saponins (Changkil saponins: CKS) on ovalbumin-induced airway inflammation in mice. CKS suppressed leukocytes number, IgE, Th1/Th2 cytokines, and MCP-1 chemokine secretion in bronchoalveolar lavage fluid. Also, ovalbumin-increased MUC5AC, MMP-2/9, and TIMP-1/-2
Matthias D Hofer et al.
Urology, 85(3), 691-697 (2015-03-04)
To describe the effects of androgens on urethral wound healing, we compared the urethral healing process in castrated Sprague-Dawley rats with and without testosterone supplementation. Of 30 castrated male Sprague-Dawley rats, 15 received testosterone cypionate (3 mg/kg; T+ rats). All
Nan-Yuan Teng et al.
Tissue engineering. Part A, 21(7-8), 1444-1454 (2015-01-28)
In vitro differentiation of stem cells into specific cell lineages provides a stable cell supply for cell therapy and tissue engineering. Therefore, understanding the mechanisms underlying such differentiation processes is critical for generating committed lineage-specific cell progenies effectively. We previously
Effat Alizadeh et al.
Chemical biology & drug design, 85(3), 268-279 (2014-07-26)
MicroRNAs are small non-coding RNAs that regulate key processes of the stem cells. Although, microRNAs have emerged as powerful regulators of differentiation, few studies have been focused on the post-transcriptional regulation of hepatic differentiation in mesenchymal stem cells (MSCs) by
Naoyuki Aihara et al.
The American journal of pathology, 185(1), 172-184 (2014-12-03)
Chronic infection by pathogens such as hepatitis C virus induces monoclonal or oligoclonal proliferation of B cells, which produce IgM rheumatoid factor, leading to the development of mixed cryoglobulinemia (MC). Antigen-driven lymphoproliferation is essential to the onset of MC; however
Carole Laffont et al.
PloS one, 10(1), e0116819-e0116819 (2015-01-07)
Cytokinins are phytohormones that regulate many developmental and environmental responses. The Medicago truncatula cytokinin receptor MtCRE1 (Cytokinin Response 1) is required for the nitrogen-fixing symbiosis with rhizobia. As several cytokinin signaling genes are modulated in roots depending on different biotic
Samantha A M Young et al.
International journal of molecular sciences, 16(10), 24732-24750 (2015-10-27)
Spermatozoa are flagellated cells whose role in fertilization is dependent on their ability to move towards an oocyte. The structure of the sperm flagella is highly conserved across species, and much of what is known about this structure is derived
Ivan Fernandez-Bueno et al.
Journal of ophthalmology, 2015, 904096-904096 (2015-12-23)
Purpose. To evaluate clinically and histologically the safety and biocompatibility of a new HDPE-based spherical porous orbital implants in rabbits. Methods. MEDPOR (Porex Surgical, Inc., Fairburn, GA, USA), OCULFIT I, and OCULFIT II (AJL Ophthalmic S.A., Vitoria, Spain) implants were
Tapasi Das et al.
Animal reproduction science, 133(3-4), 176-183 (2012-07-25)
Spermatozoal membrane proteins are considered to possess several immunological unique characteristics as the cell is formed behind the blood-testes barriers. Major goat sperm maturation antigen (SMA2) contains one hexosamine along with mannose, galactose and glucose. In the present study, effects
Shahna Shahulhameed et al.
Frontiers in immunology, 11, 154-154 (2020-03-03)
The complement system plays a crucial role in retinal homeostasis. While the proteomic analysis of ocular tissues in diabetic retinopathy (DR) has shown the deposition of complement proteins, their exact role in the pathogenesis of DR is yet unclear. We
Libo Yin et al.
Molecular medicine reports, 11(3), 1722-1732 (2014-11-15)
Cell‑based therapy is a potential alternative to liver transplantation. The goal of the present study was to examine the in vivo and in vitro hepatic differentiation potential of adipose tissue‑derived mesenchymal stem cells (AT‑MSCs) and to explore its therapeutic use.
Jared D Moretti et al.
Angewandte Chemie (International ed. in English), 51(28), 6981-6983 (2012-05-29)
In a flash: pyrotechnic incendiary formulations with good stabilities toward various ignition stimuli have been developed without the need for barium or perchlorate oxidizers. KIO(4) and NaIO(4) were introduced as pyrotechnic oxidizers and exhibited excellent pyrotechnic performance. The periodate salts
S Kirkeby
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 88(1), 19-26 (2012-09-25)
Expression of mucins in cells and tissues is of great diagnostic and prognostic importance, and immunohistochemistry frequently is used to detect them. Reports concerning mucin localization in sections sometimes are conflicting, however, partly because immunogenic regions of the mucin molecule
Gabor Szalai et al.
PloS one, 9(11), e110867-e110867 (2014-11-14)
Soluble fms-like tyrosine kinase (sFlt)-1-e15a, a primate-specific sFlt-1-isoform most abundant in the human placenta in preeclampsia, can induce preeclampsia in mice. This study compared the effects of full-length human (h)sFlt-1-e15a with those of truncated mouse (m)sFlt-1(1-3) used in previous preeclampsia
D Canbaz et al.
Allergy, 70(5), 522-532 (2015-02-14)
The initial immune response to house dust mite (HDM) is orchestrated by an interplay between epithelial cells (ECs) and dendritic cells (DCs). Innate cytokines released by HDM-exposed ECs activate airway DCs and effector inflammatory cells, which together induce a HDM-specific
Martina C Herwig et al.
Experimental eye research, 127, 243-251 (2014-08-20)
During human ocular development, expression of proteins varies in different maturation stages. This study aims to characterize structures in human fetal eyes stained by the lymphatic marker podoplanin (D2-40) with emphasis on the stage of maturation and the presence of
Alessia Amore et al.
Chembiochem : a European journal of chemical biology, 14(1), 123-131 (2013-01-03)
Incorporation of cleavable linkers into peptides and proteins is of particular value in the study of biological processes. Here we describe the synthesis of a cleavable linker that is hypersensitive to oxidative cleavage as the result of the periodate reactivity
Natalie R Lazar Adler et al.
PloS one, 10(4), e0121271-e0121271 (2015-04-02)
Burkholderia pseudomallei is the causative agent of the severe tropical disease melioidosis, which commonly presents as sepsis. The B. pseudomallei K96243 genome encodes eleven predicted autotransporters, a diverse family of secreted and outer membrane proteins often associated with virulence. In
A mild and efficient oxidation of alcohols to aldehydes and ketones with periodic acid catalyzed by chromium (III) acetylacetonate.
Xu L and Trudell ML.
Tetrahedron Letters, 44(12), 2553-2555 (2003)
Jonas Nilsson et al.
Methods in molecular biology (Clifton, N.J.), 951, 79-100 (2013-01-09)
Extracellular glycoproteins frequently carry terminal sialic acids on their N-linked and/or O-linked glycan structures. In this chapter a sialic acid specific capture-and-release protocol for the enrichment of N- and O-glycopeptides originating from glycoproteins in complex biological samples is described. The
Vaibhav Agarwal et al.
Journal of immunology (Baltimore, Md. : 1950), 191(8), 4235-4245 (2013-09-17)
Streptococcus pneumoniae (pneumococcus) is a major human pathogen, which evolved numerous successful strategies to colonize the host. In this study, we report a novel mechanism of pneumococcal-host interaction, whereby pneumococci use a host complement protein C1q, primarily involved in the
Marianne Frantzen et al.
Marine environmental research, 105, 8-19 (2015-02-01)
Concentration dependent differences in acute and long-term effects of a 48 h exposure to mechanically or chemically dispersed crude oil were assessed on juvenile lumpsucker (Cyclopterus lumpus). Acute or post-exposure mortality was only observed at oil concentrations representing higher concentrations
Francesca Polverino et al.
The American journal of pathology, 185(3), 741-755 (2014-12-30)
Small animal models of chronic obstructive pulmonary disease (COPD) have several limitations for identifying new therapeutic targets and biomarkers for human COPD. These include a pulmonary anatomy that differs from humans, the limited airway pathologies and lymphoid aggregates that develop
Mohammed Akli Ayoub et al.
PloS one, 10(3), e0119803-e0119803 (2015-03-26)
Understanding functional interactions between G protein-coupled receptors is of great physiological and pathophysiological importance. Heteromerization provides one important potential mechanism for such interaction between different signalling pathways via macromolecular complex formation. Previous studies suggested a functional interplay between angiotensin II
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