CN
405-50-5
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关键词:'405-50-5'
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Product Information Sheet -CS0740
where:
AS = Absorbance of the Test Sample at 405 nm
AB = Absorbance of the Blank at 405 nm
ASTD = Absorbance of the Standard Solution at
405 nm
Product Information Sheet - CASP3C
the
incubation overnight.
4.4. Read the Absorbance at 405 nm.
CASP3Cpis Rev 07/22
Product Information Sheet - N7653
3. After the incubation period, read the plate at
405 nm on a multiwell plate reader.
4. If the plate cannot be read immediately, add 50 L
of 3 M NaOH solution per 200 L of
Product Information Sheet - CS0780
Catalogue Number M0545
Sodium Carbonate 5 g
Catalogue Number S2127
Equipment Required but Not Provided
Spectrophotometer or ELISA reader (405 nm)
Cuvettes (3 mL, Catalogue Number C5291
EX-CELL 405 Serum-Free Medium for Insect Cells
disturbing the cell pellet.
4. Resuspend the cells in 5 mL of EX-CELLTM 405 medium.
5. Count the cells for viability and transfer to a sterile shaker
flask at a seeding density of 2-4 x 105 cells/
Product Information Sheet - SCJ4600013
0.7
Flow cytometry laser line: 360 nm, 405 nm, or 407 nm
Microscopy laser line: 405 nm
Direct replacement for: Alexa Fluor 405, Cascade
Blue, and DyLight 405
Figure 1.
Absorption and emission spectra
Insect Expression Products EX-CELL Serum-Free Media for Insect Cells
L
14420 24420
Liquid Dry Powder
14419 14421
Liquid Liquid
EX-CELLTM 405
500 mL
1000 mL
10 L
1 L
5 L
10 L
50 L
100 L
500 mL
1000 mL
10 L
Applicable Systems
Protein Level
Hydrolysate
Product Information Sheet - N2507
minutes at room temperature. The
absorbance can be read at 405 nm on a multiwell
plate reader. The reaction may be stopped by the
addition of 50 µl of 3 N NaOH per 200 µl of reaction
mixture.
Product Information Sheet - N3254
minutes at room temperature. The
absorbance can be read at 405 nm on a multiwell
plate reader. The reaction may be stopped by the
addition of 50 µl of 3 N NaOH per 200 µl of reaction
mixture.
926019 Protocol
extrusion-based bioprinting is 1 to 5 × 106 cells/mL. For example, Human dermal fibroblasts
(HDF) have been printed with TissueFab® - bioink Fibronectin Vis/405 nm at a concentration of 2 × 106 cells
Product Information Sheet - N4645
minutes at room temperature. The
absorbance can be read at 405 nm on a multiwell
plate reader. The reaction may be stopped by the
addition of 50 µl of 3 N NaOH per 200 µl of reaction
mixture.
Product Information Sheet - N6260
minutes at room
temperature. The absorbance can be read at 405 nm
on a multiwell plate reader. The reaction may be
stopped by the addition of 50 µl of 3 N NaOH per
200 µl of reaction
Product Information Sheet - MAK446
desired
temperature for 30 minutes.
3. Add 50 L of Stop Reagent to each well.
Tap plate briefly to mix.
4. Read optical density (OD) at 405 nm.
Results
1.
Product Information Sheet - SE120107
provided (i.e., 1, 2, 3, 4, and 5). For
the 405 nm readings, construct a second dose
response curve using the three calibrators with the
highest concentrations (i.e., 4, 5, and 6).
2. Assign the concentration
Product Information Sheet - MAK447
ODT4 = OD value at 405 nm of Sample at
4 minutes
ODT0 = OD value at 405 nm of Sample at
0 minutes
ODCal = OD value at 405 nm of Calibrator
ODBlank
Product Information Sheet - CS0011
reaction mix to standard wells.
3. Measure the absorbance at 405 nm (A405) in
kinetic mode at 37 °C for 60 minutes, taking
readings every 5 minutes.
4. Standard curve may be read in either
Product Information Sheet - CS0200
10 dilution 5 µL*
Substrate 200 µL 200 µL 200 µL 200 µL 200 µL 200 µL
Incubate 45 minutes at Room Temperature without shaking
Stop Solution 50
Product Information Sheet - CS0190
200 µL
Incubate 2 hours @ 37 ºC without shaking
Stop Solution 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL
Read at 405 nm
Product Information Sheet - CS1140
mixture of step 2.
4. Add ∼1 µg of Chymase (Cat. No. C8118).
5. Mix by inversion and immediately record the
increase in absorbance at 405 nm for 1 minute
(∆A405/min).
B. 100 µL
Product Information Sheet - N9389
spectrophotometrically at 405 nm. The pNPP reaction
may be stopped with the addition of 3 M NaOH
solution and read at 405 nm.
The N9389 tablets are supplied as 50 tablets (50TAB)
or 100 tablets
Product Information Sheet - MAK461
Transfer 50 L of each Sample Enzyme
dilution to wells of a clear, flat-bottom
96-well plate.
4. In triplicate, prepare Blank Control wells
that contain 50 L of enzyme buffer.
5. Initiate
Product Information Sheet - CS0230
LTC4 Conjugate 50 µL 50 µL 50 µL 50 µL
Monoclonal Anti-CysLeuk Antibody 50 µL 50 µL 50 µL
Incubate 2 hours @ RT with shaking
Wash 3X
Product Information Sheet - N2765
spectrophotometrically at 405 nm. The pNPP reaction
may be stopped with the addition of 3 M NaOH
solution and read at 405 nm.
The N2765 tablets are supplied as 50 tablets (50TAB)
or 100
Product_Information_Sheet-N3129
room temperature.
• The absorbance can be read at 405 nm on a
multiwell plate reader.
• The reaction may be stopped by adding 50 µL of
3 M NaOH per
Product Information Sheet - CASP8C
ELISA reader and measure the
initial absorbance at 405 nm (t = 0) and then
read at 5 minute intervals for t minutes (where t
can be from 20-60 minutes or even longer for
very dilute samples).
Product Information Sheet-N2640
spectrophotometrically at 405 nm. The pNPP reaction
may be stopped with the addition of 3 M NaOH
solution and read at 405 nm.
The N2640 tablets are supplied as 50 tablets (50TAB)
or 100
Product Information Sheet - SCJ4600012
labeling): 0.7
Flow cytometry laser line: 360, 405, or 407 nm
Microscopy laser line: 405 nm
Direct replacement for: Alexa Fluor 405, Cascade
Blue, and DyLight 405
Figure 1.
Absorption and emission spectra
Product Information Sheet - CS0220
100 µL
LTB4 Conjugate 50 µL 50 µL 50 µL 50 µL
Anti-LTB4 Antibody 50 µL 50 µL 50 µL
Incubate 2 hours @ RT with shaking
Wash 3X
Product Information Sheet - CS0210
200 µL
Incubate 2 hours @ 37 ºC without shaking
Stop Solution 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL
Read at 405 nm
Data Sheet - T1196
. Stop solution: 0.2 M NaOH solution
4. Water bath set to 30 °C.
5. Spectrophotometer capable of recording the
absorbance at 405 nm and appropriate cuvettes.
Procedure
The activity of TC-PTP
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