405-50-5

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定量PCR基础知识

实时聚合酶链式反应可用于计算样本的起始材料量。它相比常规PCR具有更大的检测动态范围。

PCR 实验优化和验证

PCR测定指南可指导您全程了解引物验证和其他测定优化因素，确保实现理想的DNA / RNA定量高灵敏度和特异性。

MSI检测和IHC

结直肠癌是大多数西方国家居民中普遍发生的疾病，仅次于肺癌。

How to Run Single Molecule Counting (SMC^®) Assays

Learn how to run a Single Molecule Counting (SMC®) assay from start to finish. View short videos that guide you through each step of running the SMC® IL-6 assay from reconstituting the standard, preparing the standard curve, preparing and dispensing

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can

Tips & Tricks | Decide Which Multiplex Assay Fits Your Research | MILLIPLEX® Assays

Need help deciding which multiplex assay fits your research? Before you start multiplexing with MILLIPLEX® multiplex kits, check out these tips for how to decide what the best MILLIPLEX® assay is for your research. Also, learn what materials are required

Multiplex Measurement of CVD Biomarkers | MILLIPLEX^® Assays

Learn about biomarkers of cardiovascular disease (CVD) and discover how multiplex CVD panels, such as MILLIPLEX® multiplex assays, are being used to increase the efficiency of biomarker discovery for CVD research.

Fast and high-resolution Analysis of Intact Therapeutic Monoclonal Antibody Trastuzumab using BIOshell™ A400 Protein C4 Column

Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.

Optimize Sample Performance with the SMC^® Plate Washer Evaluation Kit

Learn how to optimize your plate washer performance with the SMC® Plate Washer Evaluation Kit and assay precious samples with confidence.

High-Performance Semiconductor Polymers Based on Diketopyrrolopyrrole and Thienothiophene

The development of high-performance conjugated organic molecules and polymers has received widespread attention in industrial and academic research.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of

Nanodisc Technology: A Revolutionary System for Study of Membrane Proteins

Read our article about how the Nanodisc system allows for structural studies of membrane proteins.

Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode

In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

Photocrosslinkable Hydrogels for 3D Cell Culture and Tissue Engineering

Methacrylated collagen, hyaluronic acid, and gelatin (GelMA) hydrogels can be crosslinked with light and photoinitiators (Irgacure/LAP/Ruthenium), used as 3D cell culture scaffolds and bioinks for bioprinting.

Loading Controls for Western Blotting

Loading controls in western blotting application.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Single-Walled Carbon Nanohorn Properties & Applications

Single-walled carbon nanohorns(SWCNHs) - Find manufacturing, material properties, and applications of carbon nanohorns.

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

Mechanochemical Effect of Severe Plastic Deformations: Metal Alloys, Hydrides and Molecular Solids

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

Pressure-Temperature Calculator for Solvents

Use the calculator to determine the boiling point or pressure of solvents based on the Antoine Equation and see solvent attributes. View a list of products based on the solvent CAS number.

AIE Luminogens: A Family of New Materials with Multifaceted Functionalities

AIE Luminogens - Family of new materials with multifaceted functionalities. Find some representative applications of the AIEgens

TRC Library Citations

TRC 1.5 human and mouse shRNA libraries

Recent Advances in Hybrid Halide Perovskites-based Solar Cells

For several decades, the need for an environmentally sustainable and commercially viable source of energy has driven extensive research aimed at achieving high efficiency power generation systems that can be manufactured at low cost.

Immunohistochemistry: Current Applications in Skin Cancer

Immunohistochemistry (IHC) techniques and applications have greatly improved, dermatopathology is still largely based on H&E stained slides.This paper outlines ways in which IHC antibodies can be utilized for dermatopathology.