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Ewelina Waleka et al.
International journal of pharmaceutics, 579, 119188-119188 (2020-03-03)
A new nanogel/drug carrier of 100-150 nm size, based on poly(N-isopropylacrylamide-co-sodium acrylate) and degradable crosslinker (cystine derivative), was synthesized. Using the electrostatic interactions between the carboxylic groups in the polymer network and the protonated amine groups of doxorubicin it was possible
Dionis Minev et al.
Nucleic acids research, 47(22), 11956-11962 (2019-11-13)
There is increasing demand for single-stranded DNA (ssDNA) of lengths >200 nucleotides (nt) in synthetic biology, biological imaging and bionanotechnology. Existing methods to produce high-purity long ssDNA face limitations in scalability, complexity of protocol steps and/or yield. We present a
Dongfang Wang et al.
ACS applied materials & interfaces, 10(21), 18316-18326 (2018-05-08)
In this work, a novel nanosystem with a sandwich-like structure was synthesized via face-to-face combination of two pieces of waste cotton fabrics (CFs) carrying ferrous sulfide (FeS) and carboxyl-functionalized ferroferric oxide microsphere (CFFM), respectively, and the obtained nanosystem was named
Kyeongbae Min et al.
Methods (San Diego, Calif.), 174, 3-10 (2019-07-22)
Super-resolution microscopy techniques have been widely adopted in biological sciences. Recently, a new super-resolution microscopy technique, called expansion microscopy (ExM) has been developed. In this technique, biomolecules inside specimens are first labeled with fluorophores, followed by in-situ hydrogel synthesis and
Sinem K Saka et al.
Nature biotechnology, 37(9), 1080-1090 (2019-08-21)
Spatial mapping of proteins in tissues is hindered by limitations in multiplexing, sensitivity and throughput. Here we report immunostaining with signal amplification by exchange reaction (Immuno-SABER), which achieves highly multiplexed signal amplification via DNA-barcoded antibodies and orthogonal DNA concatemers generated
Mansour Haidar et al.
Autophagy, 15(6), 1051-1068 (2019-01-24)
HSPB1 (heat shock protein family B [small] member 1) is a ubiquitously expressed molecular chaperone. Most mutations in HSPB1 cause axonal Charcot-Marie-Tooth neuropathy and/or distal hereditary motor neuropathy. In this study we show that mutations in HSPB1 lead to impairment
Nardine S Abadeer et al.
ACS applied materials & interfaces, 7(44), 24915-24925 (2015-10-22)
The interface between nanoparticles and bacterial surfaces is of great interest for applications in nanomedicine and food safety. Here, we demonstrate that interactions between gold nanorods and bacterial surface molecules are governed by the nanoparticle surface coating. Polymer-coated gold nanorod
Kuo-Sheng Lee et al.
Neuron, 101(3), 421-428 (2019-01-20)
Callosal projections are thought to play a critical role in coordinating neural activity between the cerebral hemispheres in placental mammals, but the rules that govern the arrangement of callosal synapses on the dendrites of their target neurons remain poorly understood.
Yuan Tian et al.
The Journal of cell biology, 220(4) (2021-02-04)
The centrosome is the main microtubule-organizing center in animal cells. It comprises of two centrioles and the surrounding pericentriolar material. Protein organization at the outer layer of the centriole and outward has been studied extensively; however, an overall picture of
Circadian Modulation of Neurons and Astrocytes Controls Synaptic Plasticity in Hippocampal Area CA1.
John P McCauley et al.
Cell reports, 33(2), 108255-108255 (2020-10-15)
Most animal species operate according to a 24-h period set by the suprachiasmatic nucleus (SCN) of the hypothalamus. The rhythmic activity of the SCN modulates hippocampal-dependent memory, but the molecular and cellular mechanisms that account for this effect remain largely
Tobias C Kunz et al.
Frontiers in cell and developmental biology, 8, 617-617 (2020-08-08)
Mitochondria are double membrane bound organelles indispensable for biological processes such as apoptosis, cell signaling, and the production of many important metabolites, which includes ATP that is generated during the process known as oxidative phosphorylation (OXPHOS). The inner membrane contains
Kruno Vukušić et al.
Developmental cell, 56(9), 1253-1267 (2021-04-29)
Proper chromosome segregation into two future daughter cells requires the mitotic spindle to elongate in anaphase. However, although some candidate proteins are implicated in this process, the molecular mechanism that drives spindle elongation in human cells is unknown. Using combined
Afanasy V Lunin et al.
Molecules (Basel, Switzerland), 25(8) (2020-04-29)
The development of synthetic ways to fabricate nanosized materials with a well-defined shape, narrow-sized distribution, and high stability is of great importance to a rapidly developing area of nanotechnology. Here, we report an unusual reaction between amorphous two-line ferrihydrite and
Sperydon Koumarianos et al.
Physical chemistry chemical physics : PCCP, 23(1), 300-310 (2020-12-22)
We study here the role of polyelectrolyte chain length, that is number of repeat units (mers), in the competitive adsorption of a simple model polyanion, poly(acrylic acid), onto 85 nm spherical silica particles capped with a model polycation, poly(allylamine hydrochloride).
Aleksandra Krstulja et al.
Journal of chromatography. A, 1365, 12-18 (2014-09-15)
A series of imprinted polymers targeting nucleoside metabolites, prepared using a template analogue approach, are presented. These were prepared following selection of the optimum functional monomer by solution association studies using (1)H NMR titrations whereby methacrylic acid was shown to
Cori K Cahoon et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(33), E6857-E6866 (2017-08-02)
The synaptonemal complex (SC), a structure highly conserved from yeast to mammals, assembles between homologous chromosomes and is essential for accurate chromosome segregation at the first meiotic division. In Drosophila melanogaster, many SC components and their general positions within the
Tobias Grix et al.
Genes, 9(4) (2018-03-23)
Many tissue models have been developed to mimic liver-specific functions for metabolic and toxin conversion in in vitro assays. Most models represent a 2D environment rather than a complex 3D structure similar to native tissue. To overcome this issue, spheroid
Changyang Linghu et al.
Cell, 183(6), 1682-1698 (2020-11-25)
In order to analyze how a signal transduction network converts cellular inputs into cellular outputs, ideally one would measure the dynamics of many signals within the network simultaneously. We found that, by fusing a fluorescent reporter to a pair of
Huan Min et al.
Advanced materials (Deerfield Beach, Fla.), 32(31), e2000038-e2000038 (2020-07-01)
Fenton reaction-mediated oncotherapy is an emerging strategy which uses iron ions to catalytically convert endogenous hydrogen peroxide into hydroxyl radicals, the most reactive oxygen species found in biology, for efficient cancer therapy. However, Fenton reaction efficiency in tumor tissue is
Zhenlu Zhao et al.
Mikrochimica acta, 186(4), 226-226 (2019-03-09)
The authors have fabricated reduced graphene oxide nanosheets (rGO) supported with Fe3O4 nanoparticles and Ag/Au hollow nanoshells. The material was placed on a glassy carbon electrode which is shown to enable highly sensitive determination of As(III) which is first preconcentrated
Jiji Chen et al.
Nature methods, 18(6), 678-687 (2021-06-02)
We demonstrate residual channel attention networks (RCAN) for the restoration and enhancement of volumetric time-lapse (four-dimensional) fluorescence microscopy data. First we modify RCAN to handle image volumes, showing that our network enables denoising competitive with three other state-of-the-art neural networks.
Michel K Herde et al.
Cell reports, 32(12), 108182-108182 (2020-09-24)
Synaptically released glutamate is largely cleared by glutamate transporters localized on perisynaptic astrocyte processes. Therefore, the substantial variability of astrocyte coverage of individual hippocampal synapses implies that the efficacy of local glutamate uptake and thus the spatial fidelity of synaptic
Wei Lin et al.
Molecular cell, 70(1), 60-71 (2018-04-03)
Fidaxomicin is an antibacterial drug in clinical use for treatment of Clostridium difficile diarrhea. The active ingredient of fidaxomicin, lipiarmycin A3 (Lpm), functions by inhibiting bacterial RNA polymerase (RNAP). Here we report a cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme in
Marcus A Woodworth et al.
Nucleic acids research, 49(14), e82-e82 (2021-05-29)
Proper regulation of genome architecture and activity is essential for the development and function of multicellular organisms. Histone modifications, acting in combination, specify these activity states at individual genomic loci. However, the methods used to study these modifications often require
Matthias Schlichting et al.
Current biology : CB, 29(19), 3266-3276 (2019-10-01)
The circadian clock is a timekeeper but also helps adapt physiology to the outside world. This is because an essential feature of clocks is their ability to adjust (entrain) to the environment, with light being the most important signal. Whereas cryptochrome-mediated
Benjamin Liffner et al.
Microorganisms, 9(11) (2021-11-28)
The malaria parasite Plasmodium falciparum undergoes closed mitosis, which occurs within an intact nuclear envelope, and differs significantly from its human host. Mitosis is underpinned by the dynamics of microtubules and the nuclear envelope. To date, our ability to study P.
Tyler J Chozinski et al.
Scientific reports, 8(1), 10396-10396 (2018-07-12)
Although light microscopy is a powerful tool for the assessment of kidney physiology and pathology, it has traditionally been unable to resolve structures separated by less than the ~250 nm diffraction limit of visible light. Here, we report on the optimization
Daniel Josef Bell et al.
Soft matter, 17(3), 592-599 (2020-11-18)
Stimuli-responsive polymers are capable of reacting to an external trigger. We report self-regulated, enzymatically active, and pH-responsive hydrogels that show dynamic behavior without an external trigger. This is enabled by a feedback loop between the enzymatic conversion of glucose into
Da Un Jeong et al.
Biomedical materials (Bristol, England) (2020-11-06)
Excessive and prolonged neuroinflammation leads to neuronal cell death and limits functional recovery after traumatic brain injury (TBI). Dexamethasone (DX) is a steroidal anti-inflammatory agent that is known to attenuate early expression of pro-inflammatory cytokines associated with activated microglia/macrophages. In
Gi Bum Kim et al.
Cell, 179(1), 251-267 (2019-09-21)
In situ transgenesis methods such as viruses and electroporation can rapidly create somatic transgenic mice but lack control over copy number, zygosity, and locus specificity. Here we establish mosaic analysis by dual recombinase-mediated cassette exchange (MADR), which permits stable labeling
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