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Tyler J Chozinski et al.
Scientific reports, 8(1), 10396-10396 (2018-07-12)
Although light microscopy is a powerful tool for the assessment of kidney physiology and pathology, it has traditionally been unable to resolve structures separated by less than the ~250 nm diffraction limit of visible light. Here, we report on the optimization
Davide Gambarotto et al.
Nature methods, 16(1), 71-74 (2018-12-19)
Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for
Jervis Vermal Thevathasan et al.
Nature methods, 16(10), 1045-1053 (2019-09-29)
Quantitative fluorescence and superresolution microscopy are often limited by insufficient data quality or artifacts. In this context, it is essential to have biologically relevant control samples to benchmark and optimize the quality of microscopes, labels and imaging conditions. Here, we
Emmanuelle Steib et al.
eLife, 9 (2020-09-19)
Centrioles are characterized by a nine-fold arrangement of microtubule triplets held together by an inner protein scaffold. These structurally robust organelles experience strenuous cellular processes such as cell division or ciliary beating while performing their function. However, the molecular mechanisms
Ye Li et al.
Frontiers in neural circuits, 15, 732183-732183 (2021-11-09)
Identifying the cellular origins and mapping the dendritic and axonal arbors of neurons have been century old quests to understand the heterogeneity among these brain cells. Current Brainbow based transgenic animals take the advantage of multispectral labeling to differentiate neighboring
Peter Gorilak et al.
Open biology, 11(9), 210131-210131 (2021-09-02)
Expansion microscopy (ExM) has become a powerful super-resolution method in cell biology. It is a simple, yet robust approach, which does not require any instrumentation or reagents beyond those present in a standard microscopy facility. In this study, we used
Dora Mahecic et al.
Nature methods, 17(7), 726-733 (2020-06-24)
Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for field-independent imaging (mfFIFI). By integrating mfFIFI into an
Tyler Quoc-Thai Do et al.
iScience, 23(11), 101664-101664 (2020-11-03)
Stress adaptation is exploited by cancer cells to survive and proliferate under adverse conditions. Survival pathways induced by stress are thus highly promising therapeutic targets. One key pathway involves formation of cytoplasmic stress granules, which regulate the location, stability, and
Gerlinde Lefever et al.
Materials (Basel, Switzerland), 13(22) (2020-11-21)
Superabsorbent polymers (SAPs) are known to mitigate the development of autogenous shrinkage in cementitious mixtures with a low water-to-cement ratio. Moreover, the addition of SAPs promotes the self-healing ability of cracks. A drawback of using SAPs lies in the formation
Courtney A Burger et al.
eLife, 9 (2020-05-08)
Structural changes in pre and postsynaptic neurons that accompany synapse formation often temporally and spatially overlap. Thus, it has been difficult to resolve which processes drive patterned connectivity. To overcome this, we use the laminated outer murine retina. We identify
Weng Man Chong et al.
eLife, 9 (2020-04-04)
Subdistal appendages (sDAPs) are centriolar elements that are observed proximal to the distal appendages (DAPs) in vertebrates. Despite the obvious presence of sDAPs, structural and functional understanding of them remains elusive. Here, by combining super-resolved localization analysis and CRISPR-Cas9 genetic
N Sahabandu et al.
Journal of microscopy, 276(3), 145-159 (2019-11-07)
Centrioles are vital cellular structures that organise centrosomes and cilia. Due to their subresolutional size, centriole ultrastructural features have been traditionally analysed by electron microscopy. Here we present an adaptation of magnified analysis of the proteome expansion microscopy method, to
Jui-Yang Lai
Materials science & engineering. C, Materials for biological applications, 33(7), 3704-3710 (2013-08-06)
Characterization of corneal cellular response to hydrogel materials is an important issue in ophthalmic applications. In this study, we aimed to investigate the relationship between the feed composition of 2-hydroxyethyl methacrylate (HEMA)/acrylic acid (AAc) and material compatibility towards corneal stromal
Israr Ahmad et al.
Colloids and surfaces. B, Biointerfaces, 159, 540-545 (2017-08-29)
Inhibiting amyloid β (Aβ) aggregation has drawn much attention because it is one of the main reasons for the cause of Alzheimer's disease (AD). Here we have synthesized a nanocomposite of graphene oxide-iron oxide (GOIO) and demonstrated its ability of
Nora Trinks et al.
Communications biology, 4(1), 1151-1151 (2021-10-06)
Expansion microscopy (ExM) enables super-resolution fluorescence imaging on standard microscopes by physical expansion of the sample. However, the investigation of interactions between different organisms such as mammalian and fungal cells by ExM remains challenging because different cell types require different
Polymerization of sodium acrylate in inverse-suspension stabilized by sorbitan fatty esters
Omidian H, et al.
European Polymer Journal, 39(5), 1013-1018 (2003)
Ralph Götz et al.
Nature communications, 11(1), 6173-6173 (2020-12-04)
Expansion microscopy (ExM) enables super-resolution imaging of proteins and nucleic acids on conventional microscopes. However, imaging of details of the organization of lipid bilayers by light microscopy remains challenging. We introduce an unnatural short-chain azide- and amino-modified sphingolipid ceramide, which
Peter Jan Hooikaas et al.
eLife, 9 (2020-12-22)
When a T cell and an antigen-presenting cell form an immunological synapse, rapid dynein-driven translocation of the centrosome toward the contact site leads to reorganization of microtubules and associated organelles. Currently, little is known about how the regulation of microtubule
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Baoju Wang et al.
Journal of biophotonics, 13(5), e2419-e2419 (2020-01-31)
Either modulated illumination or temporal fluctuation analysis can assist super-resolution techniques in overcoming the diffraction limit of conventional optical microscopy. As they are not contradictory to each other, an effective combination of spatial and temporal super-resolution mechanisms would further improve
Eugene A Katrukha et al.
eLife, 10 (2021-07-28)
The neuronal microtubule cytoskeleton underlies the polarization and proper functioning of neurons, amongst others by providing tracks for motor proteins that drive intracellular transport. Different subsets of neuronal microtubules, varying in composition, stability, and motor preference, are known to exist
Preparation of reactive mineral powders used for poly (sodium acrylate) composite superabsorbents
Lee W and Chen Y
Journal of Applied Polymer Science, 97(3), 855-861 (2005)
Rui Lin et al.
Nature methods, 15(4), 275-278 (2018-02-27)
Immunosignal hybridization chain reaction (isHCR) combines antibody-antigen interactions with hybridization chain reaction (HCR) technology, which results in amplification of immunofluorescence signals by up to two to three orders of magnitude with low background. isHCR's highly modular and easily adaptable design
Danielle Bruen et al.
Macromolecular rapid communications, 41(9), e1900610-e1900610 (2020-02-25)
The ability of boronic acids (BAs) to reversibly bind diols, such as sugars, has been widely studied in recent years. In solution, through the incorporation of additional fluorophores, the BA-sugar interaction can be monitored by changes in fluorescence. Ultimately, a
Yanlu Chen et al.
Cell reports, 33(5), 108349-108349 (2020-11-05)
We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues with micron-scale (4 × 4 × 10 μm3) to submicron-scale (0.3 × 0.3 × 1 μm3) spatial resolution. The resolving ability is improved to sub-100 nm
Yohei Katoh et al.
Molecular biology of the cell, 31(20), 2195-2206 (2020-07-30)
Primary cilia are microtubule-based protrusions from the cell surface that are approximately 0.3 µm in diameter and 3 µm in length. Because size approximates the optical diffraction limit, ciliary structures at the subdiffraction level can be observed only by using
Octavian Bucur et al.
Frontiers in medicine, 5, 322-322 (2018-12-07)
Kidney glomerular diseases, such as the minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS), and other nephrotic syndromes, are typically diagnosed or confirmed via electron microscopy. Although optical microscopy has been a vital tool to examine clinical specimens for
Ralph Götz et al.
Frontiers in microbiology, 11, 574-574 (2020-04-23)
Super-resolution microscopy has evolved as a powerful method for subdiffraction-resolution fluorescence imaging of cells and cellular organelles, but requires sophisticated and expensive installations. Expansion microscopy (ExM), which is based on the physical expansion of the cellular structure of interest, provides
Akitoshi Inoue et al.
STAR protocols, 1(3), 100166-100166 (2020-12-31)
This protocol describes BATTLE-1EX, which is a combined method of BATTLE-1 and expansion microscopy to obtain high-resolution imaging of whole synaptic structures and their components of hippocampal neural circuits. BATTLE-1 uses two genetically engineered recombinase proteins and competition between two
Jean-Baptiste Raina et al.
Nature, 502(7473), 677-680 (2013-10-25)
Globally, reef-building corals are the most prolific producers of dimethylsulphoniopropionate (DMSP), a central molecule in the marine sulphur cycle and precursor of the climate-active gas dimethylsulphide. At present, DMSP production by corals is attributed entirely to their algal endosymbiont, Symbiodinium.
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