CN
Search Within
408220
应用筛选条件
关键词:'408220'
显示 31-60 共 114 条结果 关于 "408220" 范围 论文
Nardine S Abadeer et al.
ACS applied materials & interfaces, 7(44), 24915-24925 (2015-10-22)
The interface between nanoparticles and bacterial surfaces is of great interest for applications in nanomedicine and food safety. Here, we demonstrate that interactions between gold nanorods and bacterial surface molecules are governed by the nanoparticle surface coating. Polymer-coated gold nanorod
Kyeongbae Min et al.
Methods (San Diego, Calif.), 174, 3-10 (2019-07-22)
Super-resolution microscopy techniques have been widely adopted in biological sciences. Recently, a new super-resolution microscopy technique, called expansion microscopy (ExM) has been developed. In this technique, biomolecules inside specimens are first labeled with fluorophores, followed by in-situ hydrogel synthesis and
Wei Lin et al.
Molecular cell, 70(1), 60-71 (2018-04-03)
Fidaxomicin is an antibacterial drug in clinical use for treatment of Clostridium difficile diarrhea. The active ingredient of fidaxomicin, lipiarmycin A3 (Lpm), functions by inhibiting bacterial RNA polymerase (RNAP). Here we report a cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme in
Kuo-Sheng Lee et al.
Neuron, 101(3), 421-428 (2019-01-20)
Callosal projections are thought to play a critical role in coordinating neural activity between the cerebral hemispheres in placental mammals, but the rules that govern the arrangement of callosal synapses on the dendrites of their target neurons remain poorly understood.
Kruno Vukušić et al.
Developmental cell, 56(9), 1253-1267 (2021-04-29)
Proper chromosome segregation into two future daughter cells requires the mitotic spindle to elongate in anaphase. However, although some candidate proteins are implicated in this process, the molecular mechanism that drives spindle elongation in human cells is unknown. Using combined
Marcus A Woodworth et al.
Nucleic acids research, 49(14), e82-e82 (2021-05-29)
Proper regulation of genome architecture and activity is essential for the development and function of multicellular organisms. Histone modifications, acting in combination, specify these activity states at individual genomic loci. However, the methods used to study these modifications often require
Sperydon Koumarianos et al.
Physical chemistry chemical physics : PCCP, 23(1), 300-310 (2020-12-22)
We study here the role of polyelectrolyte chain length, that is number of repeat units (mers), in the competitive adsorption of a simple model polyanion, poly(acrylic acid), onto 85 nm spherical silica particles capped with a model polycation, poly(allylamine hydrochloride).
Mansour Haidar et al.
Autophagy, 15(6), 1051-1068 (2019-01-24)
HSPB1 (heat shock protein family B [small] member 1) is a ubiquitously expressed molecular chaperone. Most mutations in HSPB1 cause axonal Charcot-Marie-Tooth neuropathy and/or distal hereditary motor neuropathy. In this study we show that mutations in HSPB1 lead to impairment
Aleksandra Krstulja et al.
Journal of chromatography. A, 1365, 12-18 (2014-09-15)
A series of imprinted polymers targeting nucleoside metabolites, prepared using a template analogue approach, are presented. These were prepared following selection of the optimum functional monomer by solution association studies using (1)H NMR titrations whereby methacrylic acid was shown to
Ewelina Waleka et al.
International journal of pharmaceutics, 579, 119188-119188 (2020-03-03)
A new nanogel/drug carrier of 100-150 nm size, based on poly(N-isopropylacrylamide-co-sodium acrylate) and degradable crosslinker (cystine derivative), was synthesized. Using the electrostatic interactions between the carboxylic groups in the polymer network and the protonated amine groups of doxorubicin it was possible
Jiji Chen et al.
Nature methods, 18(6), 678-687 (2021-06-02)
We demonstrate residual channel attention networks (RCAN) for the restoration and enhancement of volumetric time-lapse (four-dimensional) fluorescence microscopy data. First we modify RCAN to handle image volumes, showing that our network enables denoising competitive with three other state-of-the-art neural networks.
Cori K Cahoon et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(33), E6857-E6866 (2017-08-02)
The synaptonemal complex (SC), a structure highly conserved from yeast to mammals, assembles between homologous chromosomes and is essential for accurate chromosome segregation at the first meiotic division. In Drosophila melanogaster, many SC components and their general positions within the
Matthias Schlichting et al.
Current biology : CB, 29(19), 3266-3276 (2019-10-01)
The circadian clock is a timekeeper but also helps adapt physiology to the outside world. This is because an essential feature of clocks is their ability to adjust (entrain) to the environment, with light being the most important signal. Whereas cryptochrome-mediated
Afanasy V Lunin et al.
Molecules (Basel, Switzerland), 25(8) (2020-04-29)
The development of synthetic ways to fabricate nanosized materials with a well-defined shape, narrow-sized distribution, and high stability is of great importance to a rapidly developing area of nanotechnology. Here, we report an unusual reaction between amorphous two-line ferrihydrite and
Michel K Herde et al.
Cell reports, 32(12), 108182-108182 (2020-09-24)
Synaptically released glutamate is largely cleared by glutamate transporters localized on perisynaptic astrocyte processes. Therefore, the substantial variability of astrocyte coverage of individual hippocampal synapses implies that the efficacy of local glutamate uptake and thus the spatial fidelity of synaptic
Benjamin Liffner et al.
Microorganisms, 9(11) (2021-11-28)
The malaria parasite Plasmodium falciparum undergoes closed mitosis, which occurs within an intact nuclear envelope, and differs significantly from its human host. Mitosis is underpinned by the dynamics of microtubules and the nuclear envelope. To date, our ability to study P.
Tobias C Kunz et al.
Frontiers in cell and developmental biology, 8, 617-617 (2020-08-08)
Mitochondria are double membrane bound organelles indispensable for biological processes such as apoptosis, cell signaling, and the production of many important metabolites, which includes ATP that is generated during the process known as oxidative phosphorylation (OXPHOS). The inner membrane contains
Yuan Tian et al.
The Journal of cell biology, 220(4) (2021-02-04)
The centrosome is the main microtubule-organizing center in animal cells. It comprises of two centrioles and the surrounding pericentriolar material. Protein organization at the outer layer of the centriole and outward has been studied extensively; however, an overall picture of
Tobias Grix et al.
Genes, 9(4) (2018-03-23)
Many tissue models have been developed to mimic liver-specific functions for metabolic and toxin conversion in in vitro assays. Most models represent a 2D environment rather than a complex 3D structure similar to native tissue. To overcome this issue, spheroid
Gi Bum Kim et al.
Cell, 179(1), 251-267 (2019-09-21)
In situ transgenesis methods such as viruses and electroporation can rapidly create somatic transgenic mice but lack control over copy number, zygosity, and locus specificity. Here we establish mosaic analysis by dual recombinase-mediated cassette exchange (MADR), which permits stable labeling
Huan Min et al.
Advanced materials (Deerfield Beach, Fla.), 32(31), e2000038-e2000038 (2020-07-01)
Fenton reaction-mediated oncotherapy is an emerging strategy which uses iron ions to catalytically convert endogenous hydrogen peroxide into hydroxyl radicals, the most reactive oxygen species found in biology, for efficient cancer therapy. However, Fenton reaction efficiency in tumor tissue is
Ka I Lee et al.
International journal of biological macromolecules, 95, 826-832 (2016-11-29)
Silk was modified via in situ polymerization of two monomers acrylamide and sodium acrylate by swelling in an effective LiBr dissolution system. Swelling of natural silks in LiBr solutions of low concentration was clearly observed under optical microscope, and their
Oane J Gros et al.
Molecular biology of the cell, 32(9), 855-868 (2021-03-11)
T-cells massively restructure their internal architecture upon reaching an antigen-presenting cell (APC) to form the immunological synapse (IS), a cell-cell interface necessary for efficient elimination of the APC. This reorganization occurs through tight coordination of cytoskeletal processes: actin forms a
Jose D Delgado et al.
Biomaterials science, 7(8), 3213-3225 (2019-06-04)
The composition of the layer of proteins adsorbed to macro- or microscopic surfaces of synthetic origin influences the response of living systems to these materials. This adsorbed layer of proteins usually comprises a "soft" coating or corona of labile or
Fabian U Zwettler et al.
Nature communications, 11(1), 3222-3222 (2020-06-28)
The synaptonemal complex (SC) is a meiosis-specific nuclear multiprotein complex that is essential for proper synapsis, recombination and segregation of homologous chromosomes. We combined structured illumination microscopy (SIM) with different expansion microscopy (ExM) protocols including U-ExM, proExM, and magnified analysis
Ali Fakhri et al.
Journal of photochemistry and photobiology. B, Biology, 169, 21-26 (2017-03-04)
Nano-medicine is a breakthrough discovery in the healthcare sector. Doxycycline is a new generation antibiotic which is proved to be a boon in the treatment of patients with complicated skin infections. We have tried to explore the benefits of synthesized
Lingyan Wu et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 17(21), 3515-3523 (2016-08-10)
Heterogeneous reactions have a vital role in the atmosphere due to their significant effects on the evolution of atmospheric aerosols, which in turn contribute to air pollution. However, the mechanism and kinetics of these processes involving unsaturated organic acids, important
Yoshinori Aso et al.
eLife, 8 (2019-11-15)
Animals employ diverse learning rules and synaptic plasticity dynamics to record temporal and statistical information about the world. However, the molecular mechanisms underlying this diversity are poorly understood. The anatomically defined compartments of the insect mushroom body function as parallel
Dong Kong et al.
Methods in molecular biology (Clifton, N.J.), 2329, 249-263 (2021-06-05)
Expansion microscopy is an imaging method based on isotropic physical expansion of biological samples, which improves optical resolution and allows imaging of subresolutional cellular components by conventional microscopes. Centrioles are small microtubule-based cylindrical structures that build centrosomes and cilia, two
Chenyi Mao et al.
Science advances, 6(22), eaba4542-eaba4542 (2020-06-11)
Fluorescence microscopy is a workhorse tool in biomedical imaging but often poses substantial challenges to practitioners in achieving bright or uniform labeling. In addition, while antibodies are effective specific labels, their reproducibility is often inconsistent, and they are difficult to
2/4