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关键词:'43794'
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Mustafa Özyürek et al.
Analytica chimica acta, 750, 173-181 (2012-10-16)
A novel on-line HPLC-DTNB method was developed for the selective determination of biologically important thiols (biothiols) such as L-cysteine (Cys), glutathione (GSH), homocysteine (HCys), N-acetylcysteine (NAC), and 1,4-dithioerythritol (DTE) in pharmaceuticals and tissue homogenates. The biothiols were separated on C18
Annamaria Tonazzi et al.
Chemico-biological interactions, 203(2), 423-429 (2013-02-14)
H(2)O(2) inhibits the [(3)H]carnitine/carnitine antiport catalysed by the mitochondrial carnitine/acylcarnitine transporter reconstituted in proteoliposomes. The inhibition was reversed by dithioerythritol, N-acetylcysteine and L-cysteine. Inhibition time-dependence revealed a faster and a slower reaction stages with orders of reaction of 1.0 and
Yang-Rae Kim et al.
ACS applied materials & interfaces, 2(1), 292-295 (2010-04-02)
A strategy for the rational design of a novel colorimetric sensor based on dithioerythritol-modified gold nanoparticles for the selective recognition of Hg2+ in aqueous media is presented. This approach relies on the combination of gold nanoparticles with Hg2+ through sulfur-Hg2+-sulfur
Eva Hrabárová et al.
Chemistry & biodiversity, 7(9), 2191-2200 (2010-09-23)
Comparison of the effectiveness of antioxidant activity of three thiol compounds, D-penicillamine, reduced L-glutathione, and 1,4-dithioerythritol, expressed as a radical-scavenging capacity based on the two independent methods, namely a decolorization 2,2'-azinobis[3-ethylbenzothiazoline-6-sulfonic acid] assay and a rotational viscometry, is reported. Particular
Melanie Brunsch et al.
PloS one, 10(8), e0135616-e0135616 (2015-08-19)
The white-rot fungus Schizophyllum commune (Agaricomycetes) was used to study the cell biology of microtubular trafficking during mating interactions, when the two partners exchange nuclei, which are transported along microtubule tracks. For this transport activity, the motor protein dynein is
P Kiousi et al.
Journal of mass spectrometry : JMS, 50(12), 1409-1419 (2015-12-05)
This manuscript describes the direct detection of mesteroloe sulfo-conjugated metabolites by liquid chromatography/quadrupole/time of flight mass spectrometry (LC/Q/TOFMS) with special focus on evaluation of their retrospective detectability and their structure elucidation. A comparison of their long-term detectability, with the mesterolone
Sarah Eskreis-Winkler et al.
Journal of magnetic resonance imaging : JMRI, 42(1), 224-229 (2014-09-02)
To demonstrate the phase and quantitative susceptibility mapping (QSM) patterns created by solid and shell spatial distributions of magnetic susceptibility in multiple sclerosis (MS) lesions. Numerical simulations and experimental phantoms of solid- and shell-shaped magnetic susceptibility sources were used to
Sony Shrestha et al.
Journal of microbiology (Seoul, Korea), 47(6), 774-782 (2010-02-04)
An entomopathogenic bacterium, Xenorhabdus nematophila, induces an immunosuppression of target insects by inhibiting phospholipase A(2) (PLA(2)) activity. Recently, an immune-associated PLA(2) gene was identified from the red flour beetle, Tribolium castaneum. This study cloned this PLA(2) gene in a bacterial
Jared R Garrett et al.
Biotechnology progress, 24(5), 1085-1089 (2009-02-06)
There is an urgent need for developing a biosensor that can real-time and noninvasively determine glucose concentration within living cells. In our previous study, we have engineered a glucose indicator protein (GIP) that can provide continuous glucose monitoring through a
S Hepp et al.
Neuroscience, 152(4), 903-912 (2008-03-18)
Previously we reported that sulfhydryl (SH) modulation affects the susceptibility of rat hippocampal slices to severe hypoxia. SH-oxidation by DTNB (5,5'-dithiobis 2-nitrobenzoic acid) or H2O2 postponed the onset of hypoxia-induced spreading depression (HSD), thereby delaying the loss of neuronal function
Marina Villar et al.
Analytical and bioanalytical chemistry, 407(19), 5571-5578 (2015-05-27)
A new approach exploiting in-syringe dispersive liquid-liquid microextraction (DLLME) for (99)Tc extraction and preconcentration from biological samples, i.e., urine and saliva, and liquid residues from treated patients is presented. (99)Tc is a beta emitter with a long half-life (2.111 × 10(5) years)
Morgane Riou et al.
PloS one, 7(4), e35134-e35134 (2012-04-12)
NMDA receptors (NMDARs) form glutamate-gated ion channels that play a critical role in CNS physiology and pathology. Together with AMPA and kainate receptors, NMDARs are known to operate as tetrameric complexes with four membrane-embedded subunits associating to form a single
C Gentile et al.
European journal of nutrition, 54(5), 811-821 (2014-09-06)
Dietary approaches to control inflammatory bowel diseases (IBD) may include proanthocyanidin-rich foods. Our previous research showed that a hydrophilic extract from Sicilian pistachio nut (HPE) contains substantial amounts of proanthocyanidins and possesses anti-inflammatory activities. We studied the effects of HPE
Giovanni Candiano et al.
Analytical chemistry, 87(9), 4814-4820 (2015-04-10)
Combinatorial peptide ligand libraries (CPLLs) tend to bind complex molecules such as dyes due to their aromatic, heterocyclic, hydrophobic, and ionic nature that may affect the protein capture specificity. In this experimental work Alcian Blue 8GX, a positively charged phthalocyanine
Kosuke Kasai et al.
Applied microbiology and biotechnology, 99(16), 6693-6703 (2015-02-11)
Fish produce mucus substances as a defensive outer barrier against several bacterial infections. We have recently identified an antibacterial L-amino acid oxidase (psLAAO1) in the mucus layer of the flounder Platichthys stellate. In this study, the antibacterial protein psLAAO1 was
Paula F Campos et al.
Methods in molecular biology (Clifton, N.J.), 840, 43-49 (2012-01-13)
DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using
A simple method for stabilizing protein-sulfhydryl groups during SDS-gel electrophoresis.
L C Lane
Analytical biochemistry, 86(2), 655-664 (1978-06-01)
Serena Giuliano et al.
PloS one, 9(12), e110073-e110073 (2014-12-04)
Nasu-Hakola disease (NHD) is a recessively inherited rare disorder characterized by a combination of neuropsychiatric and bone symptoms which, while being unique to this disease, do not provide a rationale for the unambiguous identification of patients. These individuals, in fact
Andreas Bosshart et al.
Chembiochem : a European journal of chemical biology, 16(4), 592-601 (2015-02-07)
Functional promiscuity of enzymes can often be harnessed as the starting point for the directed evolution of novel biocatalysts. Here we describe the divergent morphing of an engineered thermostable variant (Var8) of a promiscuous D-tagatose epimerase (DTE) into two efficient
Tania Gamberi et al.
Molecular bioSystems, 11(6), 1653-1667 (2015-04-24)
The cellular alterations produced in cisplatin-resistant A2780 ovarian cancer cells (A2780/R) upon treatment with the cytotoxic organogold(III) complex Aubipyc were investigated in depth through a classical proteomic approach. We observed that A2780/R cell exposure to a cytotoxic concentration of Aubipyc
J Efraim Oosterink et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 998-999, 106-113 (2015-07-22)
Methionine, homocysteine, vitamins B6, B12, B9, and their metabolites are crucial co-factors and substrates for many basic biological pathways including one-carbon metabolism, and they are particularly important for brain function and development and epigenetic mechanisms. These are essential nutrients that
Sara M Long et al.
Aquatic toxicology (Amsterdam, Netherlands), 162, 54-65 (2015-03-18)
Measuring biological responses in resident biota is a commonly used approach to monitoring polluted habitats. The challenge is to choose sensitive and, ideally, stressor-specific endpoints that reflect the responses of the ecosystem. Metabolomics is a potentially useful approach for identifying
Sophie Mompelat et al.
Journal of chromatography. A, 1407, 119-129 (2015-07-15)
A robust, selective and specific liquid chromatography-high resolution mass spectrometry (LC-HRMS) method was developed for the quantification of total residues of ceftiofur, an antibiotic belonging to the 3rd generation cephalosporins in plasma, muscle and kidney of poultry. Ceftiofur and conjugates
Genevieve Holzapfel et al.
Biochemistry, 51(31), 6114-6126 (2012-08-01)
Ras GTPase cycles between its active GTP-bound form promoted by GEFs and its inactive GDP-bound form promoted by GAPs to affect the control of various cellular functions. It is becoming increasingly apparent that subtle regulation of the GTP-bound active state
A specific and sensitive assay for disulfides.
W L Zahler et al.
The Journal of biological chemistry, 243(4), 716-719 (1968-02-25)
Kenan Coyan et al.
Research in veterinary science, 89(3), 426-431 (2010-04-21)
The aim of this study was to investigate the effects of methionine and dithioerythritol, added to the Tris extender, on ram sperm motility and LPO (lipid peroxidation) and antioxidant capacities during liquid storage up to 72 h at 5°C. Ejaculates
Jolanda Neef et al.
Applied microbiology and biotechnology, 98(24), 10131-10141 (2014-09-02)
Cell surface-exposed and secreted proteins are attractive targets for vaccination against pathogenic gram-positive bacteria. To obtain sufficient amounts of such antigens, efficient protein production platforms are needed. In this study, a pipeline for the production and purification of surface-exposed and
Jun Wu et al.
Developmental cell, 13(3), 351-364 (2007-09-04)
In vertebrates, three proteins--PERK, IRE1alpha, and ATF6alpha--sense protein-misfolding stress in the ER and initiate ER-to-nucleus signaling cascades to improve cellular function. The mechanism by which this unfolded protein response (UPR) protects ER function during stress is not clear. To address
Zahra Sharifalhoseini et al.
Ultrasonics sonochemistry, 27, 466-473 (2015-07-19)
In the present study, the sono-synthesis of ZnO nanoparticles (NPs) was performed by simple, low-cost, and the environmentally friendly method. The synthesis of zinc oxide as an antibacterial agent was performed by an ultrasonic bath (low intensity) for the indirect
M N Bucak et al.
Andrologia, 44 Suppl 1, 102-109 (2011-07-07)
The aim of this study was to determine the effects of curcumin and dithioerythritol added into bull semen extender on sperm parameters, lipid peroxidation, total glutathione and antioxidant potential levels of bull spermatozoa following the freeze/thawing process. Twenty-seven ejaculates obtained
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