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Georgia de Assis Dias Alves et al.
PloS one, 14(5), e0216501-e0216501 (2019-05-09)
Cecropia obtusa is popularly used in the Amazonian region and exhibits antioxidant activity. Cosmetic formulations containing C. obtusa extract are commercially available for purchase; however, the chemical composition and the effects of the topical application of the extract are not
Ying Zhang et al.
Carbohydrate research, 346(14), 2156-2164 (2011-08-10)
A simple and efficient procedure for the fluorescent labeling of saccharides is a prerequisite step for imaging the transport of polysaccharides in living cells. We report a one-pot strategy for the fluorescent labeling of saccharides with fluorescein-5-thiosemicarbazide (FTSC), which introduces
M Havé et al.
Plant biology (Stuttgart, Germany), 17(5), 973-979 (2015-02-17)
Leaf senescence is characterised by a massive degradation of proteins in order to recycle nitrogen to other parts of the plant, such as younger leaves or developing grain/seed. Protein degradation during leaf senescence is a highly regulated process and it
Georg Urstöger et al.
PloS one, 15(2), e0228543-e0228543 (2020-02-12)
Two molecules, 7-(diethylamino)coumarin-3-carbohydrazide (DCCH) and fluorescein-5-thiosemicarbazide (FTSC) were investigated in different solvents, under varying pH conditions regarding their spectroscopic properties for the usage as a Förster Resonance Energy Transfer (FRET) pair to study the molecular interaction between cellulosic surfaces. All
Iwona Karwaciak et al.
Oncotarget, 8(33), 54243-54264 (2017-09-15)
When monocytes are recruited to inflammation/infection sites, extravasate and differentiate into macrophages, they encounter increasing levels of oxidative stress, both from exogenous and endogenous sources. In this study, we aimed to determine whether there are specific biochemical mechanisms responsible for
Luis Marte et al.
Redox biology, 28, 101305-101305 (2019-09-13)
Signaling cascades respond to specific inputs, but also require active interventions to be maintained in their basal/inactive levels in the absence of the activating signal(s). In a screen to search for protein quality control components required for wild-type tolerance to
Shinichi Nishimura et al.
PloS one, 8(12), e83716-e83716 (2014-01-05)
Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipid
Brian M Farley et al.
Molecular biology of the cell, 23(23), 4473-4483 (2012-10-05)
RNA-binding proteins (RBPs) coordinate cell fate specification and differentiation in a variety of systems. RNA regulation is critical during oocyte development and early embryogenesis, in which RBPs control expression from maternal mRNAs encoding key cell fate determinants. The Caenorhabditis elegans
One-pot fluorescent labeling of saccharides with fluorescein-5-thiosemicarbazide for imaging polysaccharides transported in living cells
Zhang Y, et al.
Carbohydrate Research, 346(14), 2156-2164 (2011)
J D Corbett et al.
Biophysical journal, 66(1), 25-30 (1994-01-01)
We have used the fluorescence photobleaching recovery technique to study the dependence on oxygen tension of the lateral mobility of fluorescently labeled band 3, the phospholipid analogue fluorescein phosphatidylethanolamine, and glycophorins in normal red blood cell membranes. Band 3 protein
Yun Bai et al.
Nucleic acids research, 41(16), 7861-7874 (2013-06-21)
The 3' untranslated region (3'UTR) of hepatitis C virus (HCV) messenger RNA stimulates viral translation by an undetermined mechanism. We identified a high affinity interaction, conserved among different HCV genotypes, between the HCV 3'UTR and the host ribosome. The 3'UTR
Deoxygenation affects fluorescence photobleaching recovery measurements of red cell membrane protein lateral mobility
Corbett JD, et al.
Biophysical Journal, 66(1), 25-30 (1994)
Hua Tian et al.
Journal of cellular and molecular medicine, 21(1), 107-120 (2016-08-12)
Oxidative stress and inflammation play important roles in the pathogenesis of cardiovascular disease (CVD). Oxidative stress-induced desialylation is considered to be a primary step in atherogenic modification, and therefore, the attenuation of oxidative stress and/or inflammatory reactions may ameliorate CVD.
John M Pagano et al.
PLoS genetics, 10(1), e1004090-e1004090 (2014-01-24)
The four-subunit Negative Elongation Factor (NELF) is a major regulator of RNA Polymerase II (Pol II) pausing. The subunit NELF-E contains a conserved RNA Recognition Motif (RRM) and is proposed to facilitate Poll II pausing through its association with nascent
Sheng Liu et al.
RNA (New York, N.Y.), 22(12), 1828-1835 (2016-11-18)
A critical step in the HIV-1 lifecycle involves reverse transcription of the viral genomic RNA (gRNA). Human tRNA
Carina C Clingman et al.
eLife, 3 (2014-06-18)
Gene expression and metabolism are coupled at numerous levels. Cells must sense and respond to nutrients in their environment, and specialized cells must synthesize metabolic products required for their function. Pluripotent stem cells have the ability to differentiate into a
Injectable hydrogel-incorporated cancer cell-specific cisplatin releasing nanogels for targeted drug delivery
Gil MS, et al.
Journal of Material Chemistry B: Materials for Biology and Medicine, 5(34), 7140-7152 (2017)
Brian C Avanzino et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(36), 9611-9616 (2017-08-23)
Picornaviruses use internal ribosome entry sites (IRESs) to translate their genomes into protein. A typical feature of these IRESs is their ability to bind directly to the eukaryotic initiation factor (eIF) 4G component of the eIF4F cap-binding complex. Remarkably, the
Ubirajara Agero et al.
Biomedical engineering online, 9, 19-19 (2010-04-01)
We present measurements of the bulk Young's moduli of early chick embryos at Hamburger-Hamilton stage 10. Using a micropipette probe with a force constant k approximately 0.025 N/m, we applied a known force in the plane of the embryo in
Lucía Méndez et al.
Marine drugs, 18(1) (2020-01-08)
Diet-induced obesity has been linked to metabolic disorders such as cardiovascular diseases andtype 2 diabetes. A factor linking diet to metabolic disorders is oxidative stress, which can damagebiomolecules, especially proteins. The present study was designed to investigate the effect of
Klara Post et al.
Retrovirology, 13(1), 89-89 (2016-12-31)
The nucleocapsid (NC) domain of HIV-1 Gag is responsible for specific recognition and packaging of genomic RNA (gRNA) into new viral particles. This occurs through specific interactions between the Gag NC domain and the Psi packaging signal in gRNA. In
Vimal Kumar Semwal et al.
Physiology and molecular biology of plants : an international journal of functional plant biology, 24(4), 591-604 (2018-07-26)
Reproductive sinks regulate monocarpic senescence in wheat as desinking delayed flag leaf senescence under irrigated condition. In this study, wheat cv. HW 2041 and its isonuclear male sterile line (CMS) were subjected to post-anthesis water deficit stress to understand the
Sven Johannsson et al.
Scientific reports, 8(1), 8880-8880 (2018-06-13)
Dnmt2 methylates cytosine at position 38 of tRNAAsp in a variety of eukaryotic organisms. A correlation between the presence of the hypermodified nucleoside queuosine (Q) at position 34 of tRNAAsp and the Dnmt2 dependent C38 methylation was recently found in
Mariko Yokota et al.
Experimental dermatology, 23 Suppl 1, 27-31 (2014-09-23)
Moisturization of the skin plays an important role in maintaining skin homeostasis. Although it is understood that skin dryness initiates the formation of fine wrinkles, there are few objective reports to support that understanding. The purpose of this study was
Kahina Amrouche et al.
Journal of immunology (Baltimore, Md. : 1950), 202(7), 1970-1980 (2019-02-13)
The control of the activities of regulatory B (Breg) cells in immune disorders is an emerging therapeutic strategy for the recovery of immune homeostasis. Manipulating B cells using numerous drugs in vivo affect their regulatory functions, although a direct link
Gavin Pharaoh et al.
Molecular neurobiology, 57(3), 1317-1331 (2019-11-17)
Age-related decline in circulating levels of insulin-like growth factor (IGF)-1 is associated with reduced cognitive function, neuronal aging, and neurodegeneration. Decreased mitochondrial function along with increased reactive oxygen species (ROS) and accumulation of damaged macromolecules are hallmarks of cellular aging.
Taeko Mizutani et al.
Experimental dermatology, 23 Suppl 1, 23-26 (2014-09-23)
It is well known that the Camellia japonica leaf exhibits antioxidant activity because of its high content of polyphenolic compounds. Thus, the extract prepared from mature leaves of C. japonica (CJML) has been widely used as an anti-ageing material in foods
Sílvia Santos Pedrosa et al.
Macromolecular bioscience, 16(11), 1610-1620 (2016-07-28)
Hyaluronic acid nanogel (HyA-AT) is a redox sensitive crosslinkable nanogel, obtained through the conjugation of a thiolated hydrophobic molecule to the hyaluronic acid chain. Engineered nanogel was studied for its biocompatibility, including immunocompatibility and hemocompatability. The nanogel did not compromise
Alena Rajnochová Svobodová et al.
Archives of dermatological research, 311(6), 477-490 (2019-05-13)
The exposure of naked unprotected skin to solar radiation may result in numerous acute and chronic undesirable effects. Evidence suggests that silymarin, a standardized extract from Silybum marianum (L.) Gaertn. seeds, and its major component silybin suppress UVB-induced skin damage.
Daniel P Morse et al.
Journal of biotechnology, 272-273, 22-32 (2018-03-09)
Riboswitches are RNA elements found in non-coding regions of messenger RNAs that regulate gene expression through a ligand-triggered conformational change. Riboswitches typically bind tightly and specifically to their ligands, so they have the potential to serve as highly effective sensors
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