CN
Search Within
476870
应用筛选条件
关键词:'476870'
显示 1-30 共 96 条结果 关于 "476870" 范围 论文
Maria Stella Ritorto et al.
Nature communications, 5, 4763-4763 (2014-08-28)
Deubiquitylases (DUBs) are key regulators of the ubiquitin system which cleave ubiquitin moieties from proteins and polyubiquitin chains. Several DUBs have been implicated in various diseases and are attractive drug targets. We have developed a sensitive and fast assay to
Rafael Tapia-Limonchi et al.
Islets, 6(5-6), e995997-e995997 (2015-02-07)
Homeostatic levels of nitric oxide (NO) protect efficiently against apoptotic death in both human and rodent pancreatic β cells, but the protein profile of this action remains to be determined. We have applied a 2 dimensional LC-MS-MALDI-TOF/TOF-based analysis to study
Yuko Fukuyama et al.
Rapid communications in mass spectrometry : RCM, 26(20), 2454-2460 (2012-09-15)
A liquid matrix, 3-aminoquinoline (3-AQ)/α-cyano-4-hydroxycinnamic acid (CHCA), introduced by Kolli et al. in 1996 for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), has been reported for peptides and proteins, oligonucleotides, oligosaccharides, and glycopeptides. However, it has not been validated for phosphopeptides.
Des Field et al.
Applied and environmental microbiology, 81(8), 2762-2769 (2015-02-11)
The food-borne pathogenic bacterium Listeria is known for relatively low morbidity and high mortality rates, reaching up to 25 to 30%. Listeria is a hardy organism, and its control in foods represents a significant challenge. Many naturally occurring compounds, including
Yu-Shu Huang et al.
Food chemistry, 174, 571-576 (2014-12-23)
Curcumin is the major active ingredient of turmeric and is widely used as a preservative, flavouring and colouring agent. Curcumin is a potent substance with several functions, including antioxidant, antitumor, anti-inflammatory, antimicrobial, antiparasitic, antimutagenic, chemopreventive and chemotherapeutic activities. Matrix-assisted laser
Jeong Hee Moon et al.
Journal of the American Society for Mass Spectrometry, 23(1), 162-170 (2011-11-04)
Preformed ion emission is the main assumption in one of the prevailing theories for peptide and protein ion formation in matrix-assisted laser desorption ionization (MALDI). Since salts are in preformed ion forms in the matrix-analyte mixture, they are ideal systems
Sirantha Perera et al.
Rapid communications in mass spectrometry : RCM, 26(7), 853-862 (2012-03-01)
Solid phase microextraction (SPME) use small fibers directly plunged in the solution under investigation to quickly extract and quantify by different techniques the amount of selected dissolved compounds. Biological materials, peptides or proteins are accurately identified by matrix-assisted laser desorption/ionization
Ayodele Alaiya et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 30(1), 62-70 (2014-06-11)
There have been several attempts to standardize the definition and increase reproducibility in classifying lupus nephritis (LN). The last was made by the International Society of Nephrology and Renal Pathology Society in 2003 where the introduction of Class IV subcategories
Li Lin et al.
Dalton transactions (Cambridge, England : 2003), 44(12), 5867-5874 (2015-02-27)
A zinc phthalocyanine (ZnPc) derivative (Zn-tri-PcNc-8) containing tri-benzonaphtho-condensed porphyrazine with one carboxylic and six diphenylphenoxy peripheral substitutions was designed and synthesized as a sensitizer for dye-sensitized solar cells (DSSCs). For the purpose of extending the absorption spectra while minimizing the
Siying Wen et al.
Proteomics, 15(21), 3688-3698 (2015-08-01)
In vitro digestion products of proteins were compared among beef, pork, chicken, and fish. Gastric and jejunal contents from the rats fed these meat proteins were also compared. Cooked pork, beef, chicken, and fish were homogenized and incubated with pepsin
Jens Soltwisch et al.
Analytical chemistry, 84(15), 6567-6576 (2012-07-19)
The laser wavelength constitutes a key parameter in ultraviolet-matrix-assisted laser desorption ionization-mass spectrometry (UV-MALDI-MS). Optimal analytical results are only achieved at laser wavelengths that correspond to a high optical absorption of the matrix. In the presented work, the wavelength dependence
Jan Gorka et al.
Journal of the American Society for Mass Spectrometry, 23(11), 1949-1954 (2012-09-21)
Graphite as MALDI matrix or in combination with other substances has been reported in recent years. Here, we demonstrate that graphite can be used as target coating supporting the crystallization of the α-cyano-4-hydroxycinnamic acid matrix. A conventional dried-droplet preparation of
Catarina Carneiro et al.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 89, 190-200 (2014-12-17)
We describe the preparation and characterization of DODAB:MO-based liposomes and demonstrate their adjuvant potential and use in antigen delivery. Liposomes loaded with Candida albicans proteins assembled as stable negatively charged spherical nanoparticles with a mean size of 280 nm. High
Christopher A Mitchell et al.
Lipids in health and disease, 14, 84-84 (2015-08-06)
Mass spectrometry imaging (MSI) is a powerful tool for the study of intact tissue sections. Here, its application to the study of the distribution of lipids in sections of reconstructed living skin equivalents during their development and maturation is described.
Kyoung-Jin Kim et al.
Biotechnology progress, 31(3), 840-848 (2015-04-03)
Mass spectrometry (MS) analysis combined with stable isotopic labeling is a promising method for the relative quantification of aberrant glycosylation in diseases and disorders. We developed a stable isotopic labeling-based quantitative targeted glycomics (i-QTaG) technique for the comparative and quantitative
H Ando et al.
Journal of applied microbiology, 118(4), 873-880 (2015-01-30)
To evaluate the antimicrobial properties of the main Ginjo-flavour components of sake, volatile isoamyl acetate and isoamyl alcohol. Volatile isoamyl acetate and isoamyl alcohol both inhibited growth of the five yeast and 10 bacterial test strains. The minimum inhibitory dose
Svend Dam et al.
Plant physiology, 149(3), 1325-1340 (2009-01-09)
We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean (Glycine max) and pea (Pisum sativum), Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological
Lie Min et al.
Electrophoresis, 36(15), 1690-1698 (2015-04-18)
This work presents improved protease digestion conditions for membrane protein detection. The enzymatic digest of bacteriorhodopsin (BR), a model membrane protein with seven transmembrane domains (TMDs) was investigated. An initial in-gel digestion identified 17% BR sequence coverage, including part of
Hidenori Inoue et al.
Cornea, 34(9), 1105-1109 (2015-05-15)
Moraxella species are rare causative pathogens of severe sight-threatening keratitis. The aim of this study was to analyze the clinical presentation, predisposing risk factors, in vitro antimicrobial susceptibility, and treatment associated with Moraxella keratitis. We retrospectively reviewed 30 culture-proven cases
S I R Videira et al.
Persoonia, 34, 50-64 (2015-08-05)
The genus Ramularia includes numerous phytopathogenic species, several of which are economically important. Ramularia eucalypti is currently the only species of this genus known to infect Eucalyptus by causing severe leaf-spotting symptoms on this host. However, several isolates identified as
J Xu et al.
Cell death & disease, 6, e1616-e1616 (2015-01-23)
The selenoprotein thioredoxin reductase 1 (TrxR1) has several key roles in cellular redox systems and reductive pathways. Here we discovered that an evolutionarily conserved and surface-exposed tryptophan residue of the enzyme (Trp114) is excessively reactive to oxidation and exerts regulatory
Clifton K Fagerquist et al.
Journal of the American Society for Mass Spectrometry, 23(12), 2102-2114 (2012-10-12)
We previously reported the apparent formation of matrix adducts of 3,5-dimethoxy-4-hydroxy-cinnamic acid (sinapinic acid or SA) via covalent attachment to disulfide bond-containing proteins (HdeA, Hde, and YbgS) from bacterial cell lysates ionized by matrix-assisted laser desorption/ionization (MALDI) time-of-flight-time-of-flight tandem mass
Robert Erne et al.
Analytical chemistry, 91(6), 4132-4139 (2019-03-01)
Contamination is a highly controversial issue in hair analysis. Therefore, hair testing protocols typically include wash steps to remove contamination. However, recent studies claim that washing could also lead to permanent incorporation of contaminants into hair, thus questioning the validity
Si Won Kim et al.
Journal of microbiological methods, 118, 168-172 (2015-10-02)
The olive flounder (Paralichthys olivaceus) is a cultivated marine species that is economically important in Korea and Japan. Several bacterial pathogens have caused severe mortalities in farmed olive flounder, especially Streptococcus parauberis. We collected 145 S. parauberis isolates from diseased
G Di et al.
Heredity, 114(6), 564-574 (2015-02-12)
To understand the potential molecular mechanism of heterosis, protein expression patterns were compared from hybrids of Haliotis gigantea (G) and Haliotis discus hannai (D) using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight analyses. Expression differences were observed in
A Yaromina et al.
Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al], 188(5), 431-437 (2012-02-22)
High pretreatment tumor lactate content is associated with poor outcome after fractionated irradiation in human squamous cell carcinoma (hSCC) xenografts. Therefore, decreasing lactate content might be a promising approach for increasing tumor radiosensitivity. As the basis for such experiments, the
Anne R Diers et al.
The Biochemical journal, 444(3), 561-571 (2012-03-31)
Recent studies have highlighted the fact that cancer cells have an altered metabolic phenotype, and this metabolic reprogramming is required to drive the biosynthesis pathways necessary for rapid replication and proliferation. Specifically, the importance of citric acid cycle-generated intermediates in
Nida Zaidi et al.
The journal of physical chemistry. B, 118(46), 13025-13036 (2014-10-23)
Sodium dodecyl sulfate (SDS)-glycoprotein interaction serves as a model for a biological membrane. To get mechanistic insight into the interaction of SDS and glycoprotein, the effect of SDS on bovine serum fetuin (BSF) was studied in subcritical micellar concentrations at
Lucile E Wrenshall et al.
PloS one, 9(7), e102191-e102191 (2014-07-16)
Interleukin-2 (IL-2) is a multi-faceted cytokine, known for promoting proliferation, survival, and cell death depending on the cell type and state. For example, IL-2 facilitates cell death only in activated T cells when antigen and IL-2 are abundant. The availability
Hophil Min et al.
Molecules and cells, 37(6), 457-466 (2014-05-09)
Proteomic analysis is helpful in identifying cancer-associated proteins that are differentially expressed and fragmented that can be annotated as dysregulated networks and pathways during metastasis. To examine meta-static process in lung cancer, we performed a proteomics study by label-free quantitative
1/4