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Aleksandar I Mihajlović et al.
EMBO reports, 24(11), e57227-e57227 (2023-10-05)
Chromosome segregation errors in mammalian oocyte meiosis lead to developmentally compromised aneuploid embryos and become more common with advancing maternal age. Known contributors include age-related chromosome cohesion loss and spindle assembly checkpoint (SAC) fallibility in meiosis-I. But how effective the
Joaquim Javary et al.
Journal of cellular physiology, 239(3), e30938-e30938 (2022-12-29)
Extracellular signal-regulated kinase 3 (ERK3) is a poorly characterized member of the mitogen-activated protein (MAP) kinase family. Functional analysis of the ERK3 signaling pathway has been hampered by a lack of knowledge about the substrates and downstream effectors of the
Nicolás Cifuentes-Muñoz et al.
Journal of virology, 91(24) (2017-10-06)
Human metapneumovirus (HMPV) causes significant upper and lower respiratory disease in all age groups worldwide. The virus possesses a negative-sense single-stranded RNA genome of approximately 13.3 kb encapsidated by multiple copies of the nucleoprotein (N), giving rise to helical nucleocapsids.
Hieng Chiong Tie et al.
Methods in molecular biology (Clifton, N.J.), 2557, 113-125 (2022-12-14)
The Golgi complex is essential for protein transport and posttranslational modification in mammalian cells. It is critical to know the cisternal distribution of Golgi proteins to understand Golgi functions. The cis-to-trans or axial localization of a Golgi protein can be
David W Sanders et al.
eLife, 10 (2021-04-24)
Many enveloped viruses induce multinucleated cells (syncytia), reflective of membrane fusion events caused by the same machinery that underlies viral entry. These syncytia are thought to facilitate replication and evasion of the host immune response. Here, we report that co-culture
Sílvia Vale-Costa et al.
PLoS biology, 21(11), e3002290-e3002290 (2023-11-20)
It is now established that many viruses that threaten public health establish condensates via phase transitions to complete their lifecycles, and knowledge on such processes may offer new strategies for antiviral therapy. In the case of influenza A virus (IAV)
Stephen DiGiuseppe et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(22), 6289-6294 (2016-05-18)
During the entry process, the human papillomavirus (HPV) capsid is trafficked to the trans-Golgi network (TGN), whereupon it enters the nucleus during mitosis. We previously demonstrated that the minor capsid protein L2 assumes a transmembranous conformation in the TGN. Here
Helena Vandesande et al.
Journal of virology, 94(13) (2020-04-17)
Echovirus 30 (E30), a member of the enterovirus B species, is a major cause of viral meningitis, targeting children and adults alike. While it is a frequently isolated enterovirus and the cause of several outbreaks all over the world, surprisingly
Toshiyuki Habu et al.
STAR protocols, 4(1), 102063-102063 (2023-03-01)
Here, we present a protocol combining co-immunoprecipitation (Co-IP) and immunofluorescence approaches with cell cycle stage synchronization to detect cell-cycle-specific complexes. We describe steps to synchronize cells at specific cell cycle stages using drugs. We then detail the preparation of cell
Masashi Minamino et al.
Current biology : CB, 28(16), 2665-2672 (2018-08-14)
Sister chromatid cohesion, mediated by cohesin, is required for accurate chromosome segregation [1, 2]. This process requires acetylation of cohesin subunit SMC3 by evolutionarily conserved cohesin acetyltransferases: Eco1 in budding yeast; XEco1 and XEco2 in Xenopus; and ESCO1 and ESCO2 in
Cécile Jebane et al.
iScience, 26(10), 107714-107714 (2023-09-13)
Lamin A/C is a well-established key contributor to nuclear stiffness and its role in nucleus mechanical properties has been extensively studied. However, its impact on whole-cell mechanics has been poorly addressed, particularly concerning measurable physical parameters. In this study, we
Esra Unsal et al.
Chemical biology & drug design, 88(6), 783-794 (2016-11-05)
Aurora B is a serine/threonine kinase that has a central role in the regulation of mitosis. The observation of Aurora B overexpression in cancer makes it a promising target to develop antitumoral inhibitors. We describe a new potential inhibitor that
Benoit Dehapiot et al.
PLoS biology, 19(9), e3001376-e3001376 (2021-09-08)
Mammalian oocyte meiotic divisions are highly asymmetric and produce a large haploid gamete and 2 small polar bodies. This relies on the ability of the cell to break symmetry and position its spindle close to the cortex before anaphase occurs.
Visualizing the Cisternal Organization of Golgi Ministacks in HeLa Cells by Side-averaging.
Mahajan, et al.
Bio-protocol, 13, e4658-e4658 (2023)
Kozue Hamao et al.
Experimental cell research, 386(1), 111707-111707 (2019-11-07)
Cytokinesis of animal cells requires contraction of a contractile ring, composed of actin filaments and myosin II filaments. Phosphorylation of myosin II regulatory light chain (MRLC) promotes contraction of the actomyosin ring by activating myosin II motor activity. Both Rho-associated
Hao-Yang Liu et al.
Nature protocols (2022-12-01)
Single-virus tracking (SVT) offers the opportunity to monitor the journey of individual viruses in real time and to explore the interactions between viral and cellular structures in live cells, which can assist in characterizing the complex infection process and revealing
Yi-Hsuan Ho et al.
Current biology : CB, 28(16), 2673-2680 (2018-08-07)
Stress tolerance and rapid growth are often competing interests in cells. Upon severe environmental stress, many organisms activate defense systems concurrent with growth arrest. There has been debate as to whether aspects of the stress-activated transcriptome are regulated by stress
Lisa Crozier et al.
The EMBO journal, 41(6), e108599-e108599 (2022-01-18)
CDK4/6 inhibitors arrest the cell cycle in G1-phase. They are approved to treat breast cancer and are also undergoing clinical trials against a range of other tumour types. To facilitate these efforts, it is important to understand why a cytostatic
Kinetochore deterioration concommitant with centromere weakening during aging in mouse oocyte meiosis-I.
Yin, et al.
Faseb Journal, 37, e22922-e22922 (2023)
Pei Xuan Song et al.
iScience, 25(5), 104288-104288 (2022-05-17)
The accumulation of lipid droplets (LDs) in the liver is a hallmark of steatosis, which is often associated with lysosomal dysfunction. Nevertheless, the underlying mechanisms remain unclear. Here, using Huh7 cells loaded with oleate as a model to study LD
Zeynep Cansu Uretmen Kagiali et al.
Life science alliance, 3(2) (2019-12-28)
CLIC4 and CLIC1 are members of the well-conserved chloride intracellular channel proteins (CLICs) structurally related to glutathione-S-transferases. Here, we report new roles of CLICs in cytokinesis. At the onset of cytokinesis, CLIC4 accumulates at the cleavage furrow and later localizes
Hieng Chiong Tie et al.
The Journal of cell biology, 221(6) (2022-04-26)
The mammalian Golgi comprises tightly adjacent and flattened membrane sacs called cisternae. We still do not understand the molecular organization of the Golgi and intra-Golgi transport of cargos. One of the most significant challenges to studying the Golgi is resolving
Spencer A Freeman et al.
Science (New York, N.Y.), 367(6475), 301-305 (2019-12-07)
Despite ongoing (macro)pinocytosis of extracellular fluid, the volume of the endocytic pathway remains unchanged. To investigate the underlying mechanism, we used high-resolution video imaging to analyze the fate of macropinosomes formed by macrophages in vitro and in situ. Na+, the
Luis Cedeno-Rosario et al.
The Journal of biological chemistry, 298(8), 102263-102263 (2022-07-18)
Mixed lineage kinase 3 (MLK3) is a serine/threonine mitogen-activated protein kinase kinase kinase that promotes the activation of multiple mitogen-activated protein kinase pathways and is required for invasion and proliferation of ovarian cancer cells. Inhibition of MLK activity causes G2/M
DOPAL initiates I?Synuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease.
Masato, et al.
NPJ Parkinson's disease, 9, 42-42 (2023)
Stéphanie Torrino et al.
Cell metabolism, 33(7), 1342-1357 (2021-06-09)
Mechanical signals from the tumor microenvironment modulate cell mechanics and influence cell metabolism to promote cancer aggressiveness. Cells withstand external forces by adjusting the stiffness of their cytoskeleton. Microtubules (MTs) act as compression-bearing elements. Yet how cancer cells regulate MT
Archan Ganguly et al.
Neuron, 109(18), 2884-2901 (2021-09-18)
In non-neuronal cells, clathrin has established roles in endocytosis, with clathrin cages enclosing plasma membrane infoldings, followed by rapid disassembly and reuse of monomers. However, in neurons, clathrin is conveyed in slow axonal transport over days to weeks, and the
Carole Luthold et al.
Cells, 10(10) (2021-10-24)
The cochaperone BCL2-associated athanogene 3 (BAG3), in complex with the heat shock protein HSPB8, facilitates mitotic rounding, spindle orientation, and proper abscission of daughter cells. BAG3 and HSPB8 mitotic functions implicate the sequestosome p62/SQSTM1, suggesting a role for protein quality
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